Tomato leaf curl New Delhi virus-Severe

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato leaf curl New Delhi virus

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q88535|Q88535_9GEMI Capsid protein OS=Tomato leaf curl New Delhi virus-Severe OX=223351 PE=3 SV=1
MM1 pKa = 8.04WDD3 pKa = 3.6PLLHH7 pKa = 6.48EE8 pKa = 5.22FPEE11 pKa = 4.79SVHH14 pKa = 6.31GLRR17 pKa = 11.84CMLAVKK23 pKa = 9.84YY24 pKa = 10.18LQEE27 pKa = 3.83IEE29 pKa = 4.8KK30 pKa = 10.31NYY32 pKa = 10.92SPDD35 pKa = 3.24TVGYY39 pKa = 10.56DD40 pKa = 4.29LIRR43 pKa = 11.84DD44 pKa = 4.59LILVLRR50 pKa = 11.84AKK52 pKa = 10.59NYY54 pKa = 10.46GEE56 pKa = 3.8ATSRR60 pKa = 11.84YY61 pKa = 7.65HH62 pKa = 6.58HH63 pKa = 6.59FNARR67 pKa = 11.84IEE69 pKa = 4.21GTPTSQLRR77 pKa = 11.84QPLWSSCSCPHH88 pKa = 7.02CPRR91 pKa = 11.84HH92 pKa = 5.45QSKK95 pKa = 10.31GLDD98 pKa = 3.4QQADD102 pKa = 3.72EE103 pKa = 4.64QKK105 pKa = 8.49TQNVQNVV112 pKa = 3.13

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q88536|Q88536_9GEMI AC5 OS=Tomato leaf curl New Delhi virus-Severe OX=223351 PE=4 SV=1
MM1 pKa = 7.93AKK3 pKa = 9.91RR4 pKa = 11.84PADD7 pKa = 3.74IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.14SPYY27 pKa = 9.74GARR30 pKa = 11.84AVVPIARR37 pKa = 11.84VTKK40 pKa = 10.46AKK42 pKa = 10.49AWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.06SRR80 pKa = 11.84HH81 pKa = 5.57DD82 pKa = 3.47VSHH85 pKa = 6.92IGKK88 pKa = 9.26VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.89RR143 pKa = 11.84RR144 pKa = 11.84PTGSPQDD151 pKa = 3.6FGEE154 pKa = 4.31VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.6ATVTGGTYY191 pKa = 10.41ASKK194 pKa = 10.42EE195 pKa = 3.73QALVRR200 pKa = 11.84KK201 pKa = 8.27FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.61FYY251 pKa = 11.31DD252 pKa = 3.37SATNN256 pKa = 3.45

Molecular weight: 29.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1924

58

361

192.4

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.938 ± 0.576

2.287 ± 0.301

4.574 ± 0.428

4.626 ± 0.518

4.366 ± 0.379

5.301 ± 0.285

3.846 ± 0.631

5.873 ± 0.688

6.133 ± 0.421

6.653 ± 0.647

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.411

5.249 ± 0.444

5.198 ± 0.355

3.898 ± 0.6

7.173 ± 0.662

9.563 ± 1.035

5.925 ± 0.618

6.653 ± 0.618

1.455 ± 0.137

4.002 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski