Alces alces faeces associated microvirus MP12 5423
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL36|A0A2Z5CL36_9VIRU Minor capsid protein OS=Alces alces faeces associated microvirus MP12 5423 OX=2219135 PE=4 SV=1
MM1 pKa = 7.36 NKK3 pKa = 9.59 YY4 pKa = 9.73 FATNPRR10 pKa = 11.84 IKK12 pKa = 10.05 ISRR15 pKa = 11.84 TKK17 pKa = 10.25 FDD19 pKa = 4.03 LSSTHH24 pKa = 5.8 KK25 pKa = 7.65 TTFNAGYY32 pKa = 9.53 LVPIKK37 pKa = 10.2 IYY39 pKa = 10.77 EE40 pKa = 4.17 NVPGDD45 pKa = 4.02 TISVSMNSVIEE56 pKa = 4.0 MTTPLKK62 pKa = 9.78 PTMDD66 pKa = 3.75 LAVCNVYY73 pKa = 10.6 AFKK76 pKa = 11.33 VPMRR80 pKa = 11.84 LLWEE84 pKa = 4.31 HH85 pKa = 4.69 WQEE88 pKa = 4.05 MFGEE92 pKa = 4.21 NDD94 pKa = 3.2 DD95 pKa = 4.63 TYY97 pKa = 9.75 WAQPVEE103 pKa = 4.08 YY104 pKa = 9.03 TIPKK108 pKa = 8.31 LTAPTGGWSKK118 pKa = 11.3 DD119 pKa = 3.35 SIMSHH124 pKa = 5.59 MGVRR128 pKa = 11.84 MNTEE132 pKa = 4.06 GLSVSALPARR142 pKa = 11.84 AVAKK146 pKa = 10.17 IYY148 pKa = 10.91 NDD150 pKa = 3.09 WFRR153 pKa = 11.84 DD154 pKa = 3.77 QNVMTPYY161 pKa = 10.93 LEE163 pKa = 5.38 AKK165 pKa = 10.08 DD166 pKa = 3.68 DD167 pKa = 4.24 SNRR170 pKa = 11.84 EE171 pKa = 3.77 GSNGDD176 pKa = 3.38 GKK178 pKa = 10.76 NDD180 pKa = 3.3 LVAGGKK186 pKa = 9.39 CPRR189 pKa = 11.84 IAKK192 pKa = 9.63 FHH194 pKa = 7.28 DD195 pKa = 3.69 IFTTALPGPIKK206 pKa = 10.16 STEE209 pKa = 4.05 DD210 pKa = 3.11 VLLPLGEE217 pKa = 4.17 FAPVGTRR224 pKa = 11.84 TNVSPQLTNNTMLTWRR240 pKa = 11.84 MSNGEE245 pKa = 4.26 SLTSGTSYY253 pKa = 11.14 NITIGANDD261 pKa = 3.79 YY262 pKa = 10.07 TPTQARR268 pKa = 11.84 TDD270 pKa = 3.6 VDD272 pKa = 4.26 LGTYY276 pKa = 9.95 SADD279 pKa = 3.28 IQPKK283 pKa = 9.63 NLWADD288 pKa = 3.92 LSQATGATMQDD299 pKa = 3.07 FLYY302 pKa = 10.42 AYY304 pKa = 9.86 SLYY307 pKa = 11.42 KK308 pKa = 10.11 MFTIDD313 pKa = 3.02 ARR315 pKa = 11.84 GGTRR319 pKa = 11.84 YY320 pKa = 8.58 TEE322 pKa = 3.81 ILANHH327 pKa = 6.39 YY328 pKa = 10.64 GVISSDD334 pKa = 3.0 ARR336 pKa = 11.84 LQRR339 pKa = 11.84 SEE341 pKa = 4.16 YY342 pKa = 10.8 LGGTNFEE349 pKa = 4.51 INMVHH354 pKa = 6.39 VPQTSQTTEE363 pKa = 3.78 EE364 pKa = 4.36 SAQGYY369 pKa = 7.15 LTAYY373 pKa = 10.4 SNTAIRR379 pKa = 11.84 NKK381 pKa = 10.48 HH382 pKa = 6.06 LFTTSYY388 pKa = 11.24 DD389 pKa = 3.57 EE390 pKa = 4.02 HH391 pKa = 8.22 CYY393 pKa = 10.27 TILFAAIRR401 pKa = 11.84 TKK403 pKa = 8.55 QTYY406 pKa = 9.1 AQGIEE411 pKa = 4.06 KK412 pKa = 8.89 MWFHH416 pKa = 6.88 EE417 pKa = 4.18 EE418 pKa = 3.78 RR419 pKa = 11.84 TDD421 pKa = 3.89 FYY423 pKa = 10.58 MPTFAHH429 pKa = 6.88 IGEE432 pKa = 4.1 QPIRR436 pKa = 11.84 NRR438 pKa = 11.84 EE439 pKa = 3.97 LFAQGTDD446 pKa = 2.93 EE447 pKa = 4.64 DD448 pKa = 4.17 AQTFGFGEE456 pKa = 3.92 AFYY459 pKa = 10.28 EE460 pKa = 4.16 YY461 pKa = 8.97 RR462 pKa = 11.84 TQVDD466 pKa = 4.39 LVTGEE471 pKa = 4.41 FSPDD475 pKa = 3.28 AEE477 pKa = 4.3 NSLPSYY483 pKa = 10.39 TYY485 pKa = 10.2 TNDD488 pKa = 3.4 FEE490 pKa = 5.15 SLPTLSEE497 pKa = 3.95 EE498 pKa = 4.93 FILEE502 pKa = 4.13 TEE504 pKa = 4.41 DD505 pKa = 3.5 NVNRR509 pKa = 11.84 TIAVNSSLQDD519 pKa = 3.1 QFKK522 pKa = 11.08 ADD524 pKa = 4.07 FYY526 pKa = 11.43 FNVTAVRR533 pKa = 11.84 PLPPRR538 pKa = 11.84 SIPSLASHH546 pKa = 7.25 FF547 pKa = 4.11
Molecular weight: 61.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.861
IPC2_protein 4.94
IPC_protein 4.902
Toseland 4.825
ProMoST 5.041
Dawson 4.889
Bjellqvist 5.016
Wikipedia 4.762
Rodwell 4.8
Grimsley 4.736
Solomon 4.889
Lehninger 4.838
Nozaki 5.003
DTASelect 5.169
Thurlkill 4.813
EMBOSS 4.8
Sillero 5.067
Patrickios 3.668
IPC_peptide 4.889
IPC2_peptide 5.067
IPC2.peptide.svr19 4.998
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ08|A0A2Z5CJ08_9VIRU Nonstructural protein OS=Alces alces faeces associated microvirus MP12 5423 OX=2219135 PE=4 SV=1
MM1 pKa = 7.55 AKK3 pKa = 10.33 KK4 pKa = 9.86 IRR6 pKa = 11.84 NKK8 pKa = 10.41 ARR10 pKa = 11.84 DD11 pKa = 3.29 RR12 pKa = 11.84 RR13 pKa = 11.84 IFTRR17 pKa = 11.84 TASRR21 pKa = 11.84 MHH23 pKa = 6.68 KK24 pKa = 10.49 ANRR27 pKa = 11.84 TPRR30 pKa = 11.84 FMRR33 pKa = 11.84 GGIRR37 pKa = 11.84 LL38 pKa = 3.51
Molecular weight: 4.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.399
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.018
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.099
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1386
38
547
231.0
26.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.215 ± 1.629
1.227 ± 0.42
5.195 ± 0.479
8.081 ± 1.214
3.752 ± 0.77
5.411 ± 0.634
1.876 ± 0.295
5.628 ± 0.5
8.225 ± 2.436
6.205 ± 0.533
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.958 ± 0.306
6.349 ± 1.009
3.752 ± 0.776
4.69 ± 1.333
4.834 ± 1.219
6.205 ± 0.693
7.648 ± 1.416
4.618 ± 0.529
1.299 ± 0.219
4.834 ± 0.598
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here