Klebsiella phage ST405-OXA48phi1.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A411F007|A0A411F007_9CAUD Tail fiber assembly protein OS=Klebsiella phage ST405-OXA48phi1.1 OX=2516434 PE=3 SV=1
MM1 pKa = 7.7SDD3 pKa = 3.75DD4 pKa = 3.28IKK6 pKa = 11.42AVFDD10 pKa = 4.4SNFIATQAGSVQVFHH25 pKa = 7.0YY26 pKa = 10.6DD27 pKa = 2.91SNTRR31 pKa = 11.84EE32 pKa = 4.24FIGVEE37 pKa = 4.17DD38 pKa = 3.93IYY40 pKa = 11.76VPLGVGIPALTCLDD54 pKa = 3.88KK55 pKa = 11.28PPVQNEE61 pKa = 3.92YY62 pKa = 10.43QVAIRR67 pKa = 11.84SEE69 pKa = 4.85DD70 pKa = 3.45NSSWSVTDD78 pKa = 3.7DD79 pKa = 3.36FRR81 pKa = 11.84GITVYY86 pKa = 10.76DD87 pKa = 3.88LQTLASHH94 pKa = 7.4VITEE98 pKa = 4.81PGPIPDD104 pKa = 4.26TVTTSAPSTPYY115 pKa = 10.92DD116 pKa = 3.39KK117 pKa = 10.43WDD119 pKa = 3.3GSAWVTDD126 pKa = 3.65ADD128 pKa = 3.92AQHH131 pKa = 6.73AADD134 pKa = 4.77IAAADD139 pKa = 3.79QQKK142 pKa = 10.48KK143 pKa = 8.94EE144 pKa = 4.77LISQVTADD152 pKa = 4.05ISILQDD158 pKa = 3.21AVALNMATDD167 pKa = 3.84EE168 pKa = 4.68EE169 pKa = 4.87KK170 pKa = 10.98SQLTSLQGYY179 pKa = 8.62RR180 pKa = 11.84VLLYY184 pKa = 10.57RR185 pKa = 11.84VDD187 pKa = 3.35TSLAPDD193 pKa = 4.5IIWPVVTIQEE203 pKa = 4.4GNNVV207 pKa = 3.24

Molecular weight:
22.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A411EZR2|A0A411EZR2_9CAUD Uncharacterized protein OS=Klebsiella phage ST405-OXA48phi1.1 OX=2516434 PE=4 SV=1
MM1 pKa = 7.8AIIEE5 pKa = 4.25ISVSRR10 pKa = 11.84RR11 pKa = 11.84GKK13 pKa = 9.26GVQVRR18 pKa = 11.84TRR20 pKa = 11.84MVRR23 pKa = 11.84NDD25 pKa = 3.86DD26 pKa = 3.73DD27 pKa = 4.93SEE29 pKa = 4.35LVRR32 pKa = 11.84NTAKK36 pKa = 10.47RR37 pKa = 11.84IHH39 pKa = 6.23MSLGGFIHH47 pKa = 6.76GLVARR52 pKa = 11.84IFAKK56 pKa = 10.61VEE58 pKa = 4.23NISPSPADD66 pKa = 3.73KK67 pKa = 11.44NNIHH71 pKa = 6.73

Molecular weight:
7.92 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11294

33

712

217.2

23.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.236 ± 0.49

0.726 ± 0.085

6.446 ± 0.27

5.64 ± 0.265

3.223 ± 0.199

7.42 ± 0.404

1.647 ± 0.175

5.463 ± 0.254

4.277 ± 0.278

9.102 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.523 ± 0.154

3.976 ± 0.253

4.418 ± 0.173

4.507 ± 0.305

6.552 ± 0.331

6.897 ± 0.29

5.959 ± 0.373

6.614 ± 0.305

1.532 ± 0.13

2.842 ± 0.189

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski