Klebsiella phage ST405-OXA48phi1.1
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411F007|A0A411F007_9CAUD Tail fiber assembly protein OS=Klebsiella phage ST405-OXA48phi1.1 OX=2516434 PE=3 SV=1
MM1 pKa = 7.7 SDD3 pKa = 3.75 DD4 pKa = 3.28 IKK6 pKa = 11.42 AVFDD10 pKa = 4.4 SNFIATQAGSVQVFHH25 pKa = 7.0 YY26 pKa = 10.6 DD27 pKa = 2.91 SNTRR31 pKa = 11.84 EE32 pKa = 4.24 FIGVEE37 pKa = 4.17 DD38 pKa = 3.93 IYY40 pKa = 11.76 VPLGVGIPALTCLDD54 pKa = 3.88 KK55 pKa = 11.28 PPVQNEE61 pKa = 3.92 YY62 pKa = 10.43 QVAIRR67 pKa = 11.84 SEE69 pKa = 4.85 DD70 pKa = 3.45 NSSWSVTDD78 pKa = 3.7 DD79 pKa = 3.36 FRR81 pKa = 11.84 GITVYY86 pKa = 10.76 DD87 pKa = 3.88 LQTLASHH94 pKa = 7.4 VITEE98 pKa = 4.81 PGPIPDD104 pKa = 4.26 TVTTSAPSTPYY115 pKa = 10.92 DD116 pKa = 3.39 KK117 pKa = 10.43 WDD119 pKa = 3.3 GSAWVTDD126 pKa = 3.65 ADD128 pKa = 3.92 AQHH131 pKa = 6.73 AADD134 pKa = 4.77 IAAADD139 pKa = 3.79 QQKK142 pKa = 10.48 KK143 pKa = 8.94 EE144 pKa = 4.77 LISQVTADD152 pKa = 4.05 ISILQDD158 pKa = 3.21 AVALNMATDD167 pKa = 3.84 EE168 pKa = 4.68 EE169 pKa = 4.87 KK170 pKa = 10.98 SQLTSLQGYY179 pKa = 8.62 RR180 pKa = 11.84 VLLYY184 pKa = 10.57 RR185 pKa = 11.84 VDD187 pKa = 3.35 TSLAPDD193 pKa = 4.5 IIWPVVTIQEE203 pKa = 4.4 GNNVV207 pKa = 3.24
Molecular weight: 22.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.104
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.317
Thurlkill 3.77
EMBOSS 3.897
Sillero 4.062
Patrickios 3.35
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.969
Protein with the highest isoelectric point:
>tr|A0A411EZR2|A0A411EZR2_9CAUD Uncharacterized protein OS=Klebsiella phage ST405-OXA48phi1.1 OX=2516434 PE=4 SV=1
MM1 pKa = 7.8 AIIEE5 pKa = 4.25 ISVSRR10 pKa = 11.84 RR11 pKa = 11.84 GKK13 pKa = 9.26 GVQVRR18 pKa = 11.84 TRR20 pKa = 11.84 MVRR23 pKa = 11.84 NDD25 pKa = 3.86 DD26 pKa = 3.73 DD27 pKa = 4.93 SEE29 pKa = 4.35 LVRR32 pKa = 11.84 NTAKK36 pKa = 10.47 RR37 pKa = 11.84 IHH39 pKa = 6.23 MSLGGFIHH47 pKa = 6.76 GLVARR52 pKa = 11.84 IFAKK56 pKa = 10.61 VEE58 pKa = 4.23 NISPSPADD66 pKa = 3.73 KK67 pKa = 11.44 NNIHH71 pKa = 6.73
Molecular weight: 7.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.531
IPC_protein 10.35
Toseland 11.052
ProMoST 10.994
Dawson 11.067
Bjellqvist 10.833
Wikipedia 11.345
Rodwell 11.199
Grimsley 11.096
Solomon 11.345
Lehninger 11.286
Nozaki 11.023
DTASelect 10.833
Thurlkill 11.023
EMBOSS 11.477
Sillero 11.023
Patrickios 10.994
IPC_peptide 11.345
IPC2_peptide 9.706
IPC2.peptide.svr19 9.056
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
11294
33
712
217.2
23.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.236 ± 0.49
0.726 ± 0.085
6.446 ± 0.27
5.64 ± 0.265
3.223 ± 0.199
7.42 ± 0.404
1.647 ± 0.175
5.463 ± 0.254
4.277 ± 0.278
9.102 ± 0.344
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.154
3.976 ± 0.253
4.418 ± 0.173
4.507 ± 0.305
6.552 ± 0.331
6.897 ± 0.29
5.959 ± 0.373
6.614 ± 0.305
1.532 ± 0.13
2.842 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here