Panteoa phage Phynn
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 266 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514A7I9|A0A514A7I9_9CAUD Metalloprotease OS=Panteoa phage Phynn OX=2589666 GN=68 PE=4 SV=1
MM1 pKa = 7.6 NIFEE5 pKa = 5.36 KK6 pKa = 9.83 YY7 pKa = 8.23 TAVADD12 pKa = 3.95 LAGVDD17 pKa = 3.68 EE18 pKa = 4.93 NEE20 pKa = 4.54 VFEE23 pKa = 4.77 SPHH26 pKa = 5.07 GSKK29 pKa = 10.07 WIVAIDD35 pKa = 3.93 DD36 pKa = 3.91 MGCVHH41 pKa = 6.43 VLEE44 pKa = 5.3 RR45 pKa = 11.84 PCLHH49 pKa = 7.56 DD50 pKa = 5.19 SFFEE54 pKa = 4.41 CGNSAEE60 pKa = 5.16 DD61 pKa = 2.92 IGLPFEE67 pKa = 4.77 TEE69 pKa = 3.8 NSPGVYY75 pKa = 9.56 EE76 pKa = 4.57 WICDD80 pKa = 3.02 ICYY83 pKa = 10.09 QRR85 pKa = 11.84 DD86 pKa = 3.48 WEE88 pKa = 4.33 SGYY91 pKa = 9.98 IDD93 pKa = 3.1 GWEE96 pKa = 4.02 FEE98 pKa = 4.39 VTEE101 pKa = 4.87 EE102 pKa = 4.06 KK103 pKa = 11.09 LLWTWVKK110 pKa = 10.78 DD111 pKa = 3.79 GEE113 pKa = 4.22 ADD115 pKa = 3.43 EE116 pKa = 4.61 VRR118 pKa = 11.84 NKK120 pKa = 9.82 EE121 pKa = 3.89 AA122 pKa = 4.31
Molecular weight: 14.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 4.037
IPC_protein 3.973
Toseland 3.808
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.821
Rodwell 3.808
Grimsley 3.719
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.19
Thurlkill 3.821
EMBOSS 3.834
Sillero 4.088
Patrickios 1.901
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.985
Protein with the highest isoelectric point:
>tr|A0A514A720|A0A514A720_9CAUD Uncharacterized protein OS=Panteoa phage Phynn OX=2589666 GN=224 PE=4 SV=1
MM1 pKa = 8.13 KK2 pKa = 10.13 YY3 pKa = 10.64 RR4 pKa = 11.84 NVQPYY9 pKa = 10.26 RR10 pKa = 11.84 IINLAHH16 pKa = 6.3 CEE18 pKa = 3.99 VRR20 pKa = 11.84 RR21 pKa = 11.84 KK22 pKa = 10.27 NSAINTSVEE31 pKa = 3.95 RR32 pKa = 11.84 NTNITEE38 pKa = 4.0 NTMFNYY44 pKa = 10.59 GIIFLLIALVAAALGFGFLAGVAATAAKK72 pKa = 9.82 IVFIVGIILFIASLFRR88 pKa = 11.84 GTKK91 pKa = 9.52 PRR93 pKa = 11.84 DD94 pKa = 3.4 VLL96 pKa = 3.82
Molecular weight: 10.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.78
IPC_protein 10.482
Toseland 10.511
ProMoST 10.204
Dawson 10.657
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 10.935
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.716
IPC_peptide 10.73
IPC2_peptide 9.341
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
266
0
266
55506
22
1223
208.7
23.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.082 ± 0.178
1.07 ± 0.064
6.491 ± 0.112
6.688 ± 0.185
4.118 ± 0.103
6.37 ± 0.189
1.69 ± 0.067
6.511 ± 0.115
7.079 ± 0.173
7.639 ± 0.139
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.848 ± 0.085
5.212 ± 0.129
3.641 ± 0.095
3.409 ± 0.115
4.962 ± 0.096
6.733 ± 0.139
5.967 ± 0.198
7.102 ± 0.119
1.297 ± 0.056
4.091 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here