Bacillus phage phi29 (Bacteriophage phi-29)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Picovirinae; Salasvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q38504|SSB_BPPH2 Single-stranded DNA-binding protein OS=Bacillus phage phi29 OX=10756 GN=5 PE=1 SV=1
MM1 pKa = 6.55VQNDD5 pKa = 3.87FVDD8 pKa = 4.13SYY10 pKa = 11.74DD11 pKa = 3.58VTMLLQDD18 pKa = 4.71DD19 pKa = 4.87DD20 pKa = 4.15GKK22 pKa = 11.02QYY24 pKa = 11.3YY25 pKa = 9.11EE26 pKa = 3.93YY27 pKa = 10.81HH28 pKa = 6.64KK29 pKa = 10.8GLSLSDD35 pKa = 3.86FEE37 pKa = 4.66VLYY40 pKa = 11.22GNTADD45 pKa = 4.77EE46 pKa = 4.67IIKK49 pKa = 10.58LRR51 pKa = 11.84LDD53 pKa = 3.24KK54 pKa = 11.3VLL56 pKa = 4.1

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q38503|P56_BPPH2 Protein p56 OS=Bacillus phage phi29 OX=10756 GN=0.8 PE=1 SV=1
MM1 pKa = 7.67SYY3 pKa = 10.87LVTIFSINDD12 pKa = 3.09NGVVRR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 8.19DD20 pKa = 3.15TTARR24 pKa = 11.84SRR26 pKa = 11.84TDD28 pKa = 3.05AASVLIKK35 pKa = 10.53YY36 pKa = 7.51KK37 pKa = 10.72APRR40 pKa = 11.84GYY42 pKa = 10.74KK43 pKa = 9.37FDD45 pKa = 3.84RR46 pKa = 11.84FEE48 pKa = 4.34LSRR51 pKa = 11.84EE52 pKa = 3.77KK53 pKa = 10.44TIFDD57 pKa = 3.32II58 pKa = 5.4

Molecular weight: 6.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

2

29

6249

31

854

215.5

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.761 ± 0.395

0.704 ± 0.155

6.417 ± 0.405

6.737 ± 0.549

4.385 ± 0.316

6.673 ± 0.805

1.536 ± 0.151

6.817 ± 0.369

7.537 ± 0.552

8.033 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.303

6.641 ± 0.435

2.848 ± 0.161

3.361 ± 0.326

4.081 ± 0.339

6.737 ± 0.511

6.449 ± 0.515

6.897 ± 0.486

1.28 ± 0.236

4.401 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski