Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3363 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1F943|C1F943_ACIC5 Beta-glucosidase OS=Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161) OX=240015 GN=ACP_2108 PE=3 SV=1
MM1 pKa = 7.23 LTCSQDD7 pKa = 3.46 SPSSLLSFFKK17 pKa = 10.77 ARR19 pKa = 11.84 SAPSRR24 pKa = 11.84 LLLVALCSLGLVLAGCSGGSSSSGSSSTSGTAPGGGSGSGGGSNNTPTPGAANSVQLGTATVFDD88 pKa = 4.67 GPNPEE93 pKa = 4.27 PAQIAADD100 pKa = 3.61 ASGDD104 pKa = 3.79 DD105 pKa = 3.69 TLAWTDD111 pKa = 3.25 PSGQVYY117 pKa = 9.47 VSRR120 pKa = 11.84 LAAGSTSWTTPVALIPATSTLKK142 pKa = 10.52 AHH144 pKa = 6.89 GGITFAASDD153 pKa = 3.51 ASGNTTVLFQASDD166 pKa = 3.42 ASFQSYY172 pKa = 9.47 LVASTLLAGSSKK184 pKa = 9.7 WSQPATLSIPDD195 pKa = 3.83 ASGSAATLMSNGDD208 pKa = 2.8 IAAAYY213 pKa = 9.37 GGSLTDD219 pKa = 3.63 SVQVATFAPQTQSWGAPVTAAQSPTLLGNFTITSTPQNALTVLWTANIVPNNDD272 pKa = 3.47 PSQQSASGVYY282 pKa = 9.85 AATASGSAGPFSTPVEE298 pKa = 4.01 IDD300 pKa = 3.49 VQPGGTAPGIVTSLPDD316 pKa = 3.53 AVTDD320 pKa = 3.65 TSGNTTAIWTASPQAGGATDD340 pKa = 5.18 LYY342 pKa = 11.03 ASRR345 pKa = 11.84 LAAGSSTWSKK355 pKa = 10.36 PVQLDD360 pKa = 3.59 TSSSNNPYY368 pKa = 10.65 VGFPTPLVVDD378 pKa = 3.96 AQGNVTVAWIAGINSPRR395 pKa = 11.84 TVQAVRR401 pKa = 11.84 YY402 pKa = 8.12 DD403 pKa = 3.34 ASTSQWSTPSLVQTLPSGTVAGIPALAIGPTQGEE437 pKa = 4.18 VSVFWNQPPSASSSGSNGDD456 pKa = 3.02 IYY458 pKa = 11.15 QSIWSSGSGSWGNPIQADD476 pKa = 4.21 LPLPTAGASAATFPVAAVNGSSSMAVAWGDD506 pKa = 3.5 PVTGSNSSYY515 pKa = 10.19 TLYY518 pKa = 11.37 ANVLQQ523 pKa = 4.54
Molecular weight: 51.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.579
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.846
Rodwell 3.656
Grimsley 3.49
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.304
Thurlkill 3.656
EMBOSS 3.846
Sillero 3.961
Patrickios 0.782
IPC_peptide 3.834
IPC2_peptide 3.923
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|C1F1H2|C1F1H2_ACIC5 Glucose-6-phosphate 1-dehydrogenase OS=Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161) OX=240015 GN=zwf1 PE=3 SV=1
MM1 pKa = 7.7 ALTRR5 pKa = 11.84 HH6 pKa = 5.36 ILLRR10 pKa = 11.84 NGAFALVGTAVIPAFLTRR28 pKa = 11.84 TLHH31 pKa = 5.83 AQTAAARR38 pKa = 11.84 AWQGAGGGVSARR50 pKa = 11.84 RR51 pKa = 11.84 GPSRR55 pKa = 3.71
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3363
0
3363
1183035
30
3102
351.8
38.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.3 ± 0.054
0.905 ± 0.013
4.752 ± 0.027
5.591 ± 0.047
3.775 ± 0.029
7.904 ± 0.043
2.588 ± 0.021
4.855 ± 0.029
3.389 ± 0.032
10.18 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.37 ± 0.021
3.222 ± 0.04
5.439 ± 0.034
4.25 ± 0.033
6.533 ± 0.044
6.09 ± 0.044
5.62 ± 0.041
7.019 ± 0.032
1.406 ± 0.019
2.811 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here