Kingella kingae ATCC 23330

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Kingella; Kingella kingae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5S6Z7|F5S6Z7_9NEIS Lipoyl synthase OS=Kingella kingae ATCC 23330 OX=887327 GN=lipA PE=3 SV=1
MM1 pKa = 7.18AQIPNHH7 pKa = 6.58LYY9 pKa = 8.96YY10 pKa = 10.15TAQHH14 pKa = 4.34QWIRR18 pKa = 11.84QNEE21 pKa = 4.35DD22 pKa = 2.36GTATVGITDD31 pKa = 4.6FAQEE35 pKa = 4.09TLGDD39 pKa = 3.63IVFVEE44 pKa = 4.58LPDD47 pKa = 3.86VGTEE51 pKa = 4.1LAANVQAASVEE62 pKa = 4.68SVKK65 pKa = 10.84SASDD69 pKa = 3.3VYY71 pKa = 11.42APVAGKK77 pKa = 10.02VVAINEE83 pKa = 4.02DD84 pKa = 4.43LIANPEE90 pKa = 4.06VANEE94 pKa = 4.06SPYY97 pKa = 10.17DD98 pKa = 3.76AGWFFVVQPNQASDD112 pKa = 2.92IATLLTAEE120 pKa = 4.38QYY122 pKa = 10.02QAEE125 pKa = 4.46TAA127 pKa = 3.58

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5S943|F5S943_9NEIS TypeIII_RM_meth domain-containing protein OS=Kingella kingae ATCC 23330 OX=887327 GN=HMPREF0476_1726 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2102

0

2102

572704

39

1832

272.5

30.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.354 ± 0.074

1.082 ± 0.018

5.111 ± 0.046

5.51 ± 0.059

4.105 ± 0.044

6.715 ± 0.051

2.426 ± 0.032

5.984 ± 0.042

5.144 ± 0.046

10.215 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.027

4.504 ± 0.052

4.049 ± 0.03

5.354 ± 0.052

4.882 ± 0.04

5.647 ± 0.045

5.313 ± 0.046

6.784 ± 0.046

1.317 ± 0.024

2.903 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski