Oreochromis niloticus (Nile tilapia) (Tilapia nilotica)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74622 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A669DE68|A0A669DE68_ORENI GLTP domain-containing protein OS=Oreochromis niloticus OX=8128 GN=gltp PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 4.34AWTDD6 pKa = 3.2GWADD10 pKa = 3.09GWMMDD15 pKa = 3.11GWMDD19 pKa = 3.17AWTDD23 pKa = 3.21GWADD27 pKa = 2.87GWMDD31 pKa = 4.24GSSLCLSHH39 pKa = 7.45SHH41 pKa = 7.45DD42 pKa = 4.1SEE44 pKa = 4.59TVSWEE49 pKa = 4.09SSVQAGNLRR58 pKa = 11.84LHH60 pKa = 6.65HH61 pKa = 6.74PSNLLTDD68 pKa = 4.5LVYY71 pKa = 10.63APPPAPPSSSTLLL84 pKa = 3.69

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A669EFX0|A0A669EFX0_ORENI Uncharacterized protein OS=Oreochromis niloticus OX=8128 PE=4 SV=1
MM1 pKa = 7.62LLLLSHH7 pKa = 7.58PPPPHH12 pKa = 6.35PQSGPQPPHH21 pKa = 7.15PPLKK25 pKa = 10.26HH26 pKa = 5.8PPPRR30 pKa = 11.84QPLNHH35 pKa = 7.27PPPCPPLKK43 pKa = 10.31YY44 pKa = 9.81PPPRR48 pKa = 11.84PPLKK52 pKa = 10.21HH53 pKa = 6.18PPPRR57 pKa = 11.84PPLKK61 pKa = 10.23HH62 pKa = 6.06PPSRR66 pKa = 11.84QPMNHH71 pKa = 6.91PPPRR75 pKa = 11.84PPLKK79 pKa = 10.28HH80 pKa = 6.09PPPRR84 pKa = 11.84QPMNHH89 pKa = 6.92PPPRR93 pKa = 11.84PPLKK97 pKa = 10.21HH98 pKa = 6.18PPPRR102 pKa = 11.84PPLKK106 pKa = 10.47HH107 pKa = 6.09PLPRR111 pKa = 11.84QPMNHH116 pKa = 6.93PPPRR120 pKa = 11.84PPLKK124 pKa = 10.23HH125 pKa = 6.06PPSRR129 pKa = 11.84QPMNHH134 pKa = 6.81PPPRR138 pKa = 11.84QPMNHH143 pKa = 6.56PPSRR147 pKa = 11.84QPMNHH152 pKa = 6.81PPPRR156 pKa = 11.84QPMNHH161 pKa = 6.91PPSPPPLKK169 pKa = 10.22HH170 pKa = 6.18PPSRR174 pKa = 11.84PPLKK178 pKa = 10.21HH179 pKa = 6.03PPSRR183 pKa = 11.84QPMNHH188 pKa = 6.81PPPRR192 pKa = 11.84QPMNHH197 pKa = 6.76LPPRR201 pKa = 11.84PPLKK205 pKa = 10.28HH206 pKa = 6.06PPSRR210 pKa = 11.84QPMNHH215 pKa = 6.91PPPRR219 pKa = 11.84PPLKK223 pKa = 10.23HH224 pKa = 6.06PPSRR228 pKa = 11.84QPMNHH233 pKa = 6.81PPPRR237 pKa = 11.84QPMNHH242 pKa = 6.69PPSRR246 pKa = 11.84PPLKK250 pKa = 10.14HH251 pKa = 6.13PPSRR255 pKa = 11.84PPLKK259 pKa = 10.14HH260 pKa = 6.13PPSRR264 pKa = 11.84PPLKK268 pKa = 10.44HH269 pKa = 5.27PTPRR273 pKa = 11.84PPLKK277 pKa = 10.28HH278 pKa = 6.09PPPRR282 pKa = 11.84QPMNHH287 pKa = 6.69PPSRR291 pKa = 11.84PPLKK295 pKa = 10.21HH296 pKa = 6.03PPSRR300 pKa = 11.84QPMNHH305 pKa = 6.91PPPRR309 pKa = 11.84PPLKK313 pKa = 10.21HH314 pKa = 6.18PPPRR318 pKa = 11.84PPLKK322 pKa = 10.28HH323 pKa = 6.09PPPRR327 pKa = 11.84QPMNHH332 pKa = 6.92PPPRR336 pKa = 11.84PPLKK340 pKa = 10.21HH341 pKa = 6.18PPPRR345 pKa = 11.84PPLKK349 pKa = 10.47HH350 pKa = 6.09PLPRR354 pKa = 11.84QPMNHH359 pKa = 6.93PPPRR363 pKa = 11.84PPLKK367 pKa = 10.23HH368 pKa = 6.06PPSRR372 pKa = 11.84QPMNHH377 pKa = 6.56PPSRR381 pKa = 11.84QPMNHH386 pKa = 7.18PPPCQPMNHH395 pKa = 6.74PPSRR399 pKa = 11.84PPLKK403 pKa = 10.22HH404 pKa = 6.16PPPRR408 pKa = 11.84PPLKK412 pKa = 10.47HH413 pKa = 6.09PLPRR417 pKa = 11.84QPMIHH422 pKa = 6.92PPPRR426 pKa = 11.84PPLKK430 pKa = 10.13HH431 pKa = 6.16PPSRR435 pKa = 11.84PPLKK439 pKa = 10.44HH440 pKa = 5.27PTPRR444 pKa = 11.84PPLKK448 pKa = 10.28HH449 pKa = 6.09PPPRR453 pKa = 11.84QPMNHH458 pKa = 6.28PQPRR462 pKa = 11.84PPLKK466 pKa = 10.25HH467 pKa = 6.18PPPRR471 pKa = 11.84PPLKK475 pKa = 10.47HH476 pKa = 6.09PLPRR480 pKa = 11.84QPMNHH485 pKa = 6.93PPPRR489 pKa = 11.84PPLKK493 pKa = 10.23HH494 pKa = 6.06PPSRR498 pKa = 11.84QPMNHH503 pKa = 7.18PPPCQPMNHH512 pKa = 6.74PPSRR516 pKa = 11.84PPLKK520 pKa = 10.14HH521 pKa = 6.13PPSRR525 pKa = 11.84PPLKK529 pKa = 10.44HH530 pKa = 5.27PTPRR534 pKa = 11.84PPLKK538 pKa = 10.28HH539 pKa = 6.09PPPRR543 pKa = 11.84QPMNHH548 pKa = 6.92PPPRR552 pKa = 11.84PPLKK556 pKa = 10.21HH557 pKa = 6.18PPPRR561 pKa = 11.84PPLKK565 pKa = 10.49HH566 pKa = 6.1PLPRR570 pKa = 11.84LPMNHH575 pKa = 7.1PPPRR579 pKa = 11.84PPLKK583 pKa = 10.23HH584 pKa = 6.06PPSRR588 pKa = 11.84QPMNHH593 pKa = 6.56PPSRR597 pKa = 11.84QPMNHH602 pKa = 7.18PPPCQPMNHH611 pKa = 6.74PPSRR615 pKa = 11.84PPLKK619 pKa = 10.22HH620 pKa = 6.16PPPRR624 pKa = 11.84PPLKK628 pKa = 10.47HH629 pKa = 6.09PLPRR633 pKa = 11.84QPMIHH638 pKa = 6.92PPPRR642 pKa = 11.84PPLKK646 pKa = 10.13HH647 pKa = 6.16PPSRR651 pKa = 11.84PPLKK655 pKa = 10.22HH656 pKa = 6.16PPPRR660 pKa = 11.84PPLKK664 pKa = 10.47HH665 pKa = 6.09PLPRR669 pKa = 11.84QPMIHH674 pKa = 6.92PPPRR678 pKa = 11.84PPLKK682 pKa = 10.13HH683 pKa = 6.16PPSRR687 pKa = 11.84PPLKK691 pKa = 10.28HH692 pKa = 5.85PPPRR696 pKa = 11.84KK697 pKa = 9.44PMNHH701 pKa = 6.82PPPRR705 pKa = 11.84PPLKK709 pKa = 10.21HH710 pKa = 6.18PPPRR714 pKa = 11.84PPLKK718 pKa = 10.28HH719 pKa = 6.18PPPRR723 pKa = 11.84QPINHH728 pKa = 7.03PPPRR732 pKa = 11.84PPLKK736 pKa = 10.21HH737 pKa = 6.18PPPRR741 pKa = 11.84PPLKK745 pKa = 10.47HH746 pKa = 6.09PLPRR750 pKa = 11.84QPMNRR755 pKa = 11.84PPPRR759 pKa = 11.84QPMNHH764 pKa = 7.13PPPCPSLKK772 pKa = 10.25HH773 pKa = 5.53PPPRR777 pKa = 11.84QPMNHH782 pKa = 6.81PPPRR786 pKa = 11.84QPMNHH791 pKa = 6.92PPPRR795 pKa = 11.84PPLKK799 pKa = 10.21HH800 pKa = 6.18PPPRR804 pKa = 11.84PPLKK808 pKa = 10.13HH809 pKa = 6.16PPSRR813 pKa = 11.84PPLKK817 pKa = 10.14HH818 pKa = 6.13PPSRR822 pKa = 11.84PPLKK826 pKa = 10.32HH827 pKa = 5.81PPSRR831 pKa = 11.84QSMNHH836 pKa = 6.02PPPRR840 pKa = 11.84QPMNHH845 pKa = 6.76LPPRR849 pKa = 11.84PPLKK853 pKa = 10.28HH854 pKa = 6.18PPPRR858 pKa = 11.84PPLKK862 pKa = 10.23HH863 pKa = 6.06PPSRR867 pKa = 11.84QPMNHH872 pKa = 6.91PPPRR876 pKa = 11.84PPLKK880 pKa = 10.26HH881 pKa = 6.46PPPPPPLKK889 pKa = 10.29HH890 pKa = 6.1PPSRR894 pKa = 11.84QPMNHH899 pKa = 7.53PPRR902 pKa = 11.84KK903 pKa = 9.16HH904 pKa = 5.97PPPRR908 pKa = 11.84PPLKK912 pKa = 10.49HH913 pKa = 6.1PLPRR917 pKa = 11.84LPMNHH922 pKa = 7.29PPPCPPLKK930 pKa = 10.25HH931 pKa = 6.2PPSRR935 pKa = 11.84QPMNHH940 pKa = 6.56PPSRR944 pKa = 11.84QPMNHH949 pKa = 6.81PPPRR953 pKa = 11.84QPMNHH958 pKa = 6.69PPSRR962 pKa = 11.84PPLKK966 pKa = 10.31HH967 pKa = 5.93PPPRR971 pKa = 11.84PSLKK975 pKa = 10.38HH976 pKa = 5.17PLPRR980 pKa = 11.84QPMIHH985 pKa = 6.92PPPRR989 pKa = 11.84PPLKK993 pKa = 10.23HH994 pKa = 6.06PPSRR998 pKa = 11.84QPMNHH1003 pKa = 6.56PPSRR1007 pKa = 11.84QPMNHH1012 pKa = 6.56PPSRR1016 pKa = 11.84QPMNHH1021 pKa = 6.81PPPRR1025 pKa = 11.84QPMKK1029 pKa = 10.14HH1030 pKa = 6.07PPSRR1034 pKa = 11.84PPLKK1038 pKa = 10.22HH1039 pKa = 6.16PPPRR1043 pKa = 11.84PPLKK1047 pKa = 10.28HH1048 pKa = 6.09PPPRR1052 pKa = 11.84QPMNHH1057 pKa = 7.35PLPHH1061 pKa = 7.2PPLKK1065 pKa = 10.22HH1066 pKa = 6.02LPPRR1070 pKa = 11.84PPLKK1074 pKa = 10.23HH1075 pKa = 6.16PPSRR1079 pKa = 11.84PPMNHH1084 pKa = 6.63PPPRR1088 pKa = 11.84QPMNHH1093 pKa = 6.76LPPRR1097 pKa = 11.84PPLKK1101 pKa = 10.28HH1102 pKa = 6.18PPPRR1106 pKa = 11.84PPLKK1110 pKa = 10.23HH1111 pKa = 6.06PPSRR1115 pKa = 11.84QPMNHH1120 pKa = 6.91PPPRR1124 pKa = 11.84PPLKK1128 pKa = 10.21HH1129 pKa = 6.18PPPRR1133 pKa = 11.84PPLKK1137 pKa = 10.27HH1138 pKa = 6.41PPSPQPMNHH1147 pKa = 6.86PPPRR1151 pKa = 11.84PPLKK1155 pKa = 10.34HH1156 pKa = 6.61PPPCHH1161 pKa = 6.36PMNHH1165 pKa = 6.84PPPRR1169 pKa = 11.84PPINHH1174 pKa = 6.98PPPRR1178 pKa = 11.84PPLKK1182 pKa = 10.28HH1183 pKa = 6.09PPPRR1187 pKa = 11.84QPLNHH1192 pKa = 7.27PPPCPPLKK1200 pKa = 10.31YY1201 pKa = 9.81PPPRR1205 pKa = 11.84PPLKK1209 pKa = 10.21HH1210 pKa = 6.18PPPRR1214 pKa = 11.84PPLKK1218 pKa = 10.21HH1219 pKa = 6.18PPPRR1223 pKa = 11.84PPLKK1227 pKa = 10.23HH1228 pKa = 6.06PPSRR1232 pKa = 11.84QPMNHH1237 pKa = 6.91PPPRR1241 pKa = 11.84PPLKK1245 pKa = 10.28HH1246 pKa = 6.15PPPRR1250 pKa = 11.84QPVNHH1255 pKa = 6.77PPSRR1259 pKa = 11.84RR1260 pKa = 11.84PMNHH1264 pKa = 6.57PPPRR1268 pKa = 11.84QHH1270 pKa = 6.82INHH1273 pKa = 6.9PHH1275 pKa = 5.89HH1276 pKa = 6.37VHH1278 pKa = 6.71LL1279 pKa = 5.27

Molecular weight: 146.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28038

46584

74622

50247682

17

8662

673.4

75.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.355 ± 0.007

2.329 ± 0.008

5.2 ± 0.006

6.755 ± 0.012

3.86 ± 0.006

6.116 ± 0.01

2.629 ± 0.005

4.764 ± 0.006

5.806 ± 0.009

9.71 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.003

4.008 ± 0.006

5.322 ± 0.01

4.623 ± 0.007

5.464 ± 0.006

8.387 ± 0.009

5.75 ± 0.013

6.444 ± 0.007

1.207 ± 0.003

2.917 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski