Firmicutes bacterium CAG:65

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6EJ78|R6EJ78_9FIRM GTP 3' 8-cyclase OS=Firmicutes bacterium CAG:65 OX=1262994 GN=moaA PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.48KK3 pKa = 10.11FLALALASVMAVSLVACGSTNADD26 pKa = 3.37TNAEE30 pKa = 3.99SSAAATTTAAATEE43 pKa = 4.42SGSEE47 pKa = 4.09EE48 pKa = 4.39PGLKK52 pKa = 9.55IAIVSSPSGVDD63 pKa = 3.24DD64 pKa = 5.18GSFNQDD70 pKa = 2.53NYY72 pKa = 11.46NGVLKK77 pKa = 10.55FIEE80 pKa = 4.56EE81 pKa = 4.22NPNATVTPIRR91 pKa = 11.84EE92 pKa = 4.18EE93 pKa = 4.24TGDD96 pKa = 3.44SAAAVQAVADD106 pKa = 4.0VVADD110 pKa = 3.57YY111 pKa = 11.33DD112 pKa = 4.73VIVCCGFQFAGIGNLAQEE130 pKa = 4.43NPDD133 pKa = 3.49TKK135 pKa = 10.83FILIDD140 pKa = 3.3SWPTIDD146 pKa = 3.79GTEE149 pKa = 3.88VTADD153 pKa = 3.36QVIDD157 pKa = 3.76NVYY160 pKa = 8.94AAYY163 pKa = 10.34YY164 pKa = 10.28AEE166 pKa = 4.23QEE168 pKa = 4.32SGFYY172 pKa = 10.71AGMAAALSTTTGKK185 pKa = 10.3VAVVNGIAYY194 pKa = 7.57PSNVNYY200 pKa = 10.29QYY202 pKa = 11.57GFEE205 pKa = 4.46CGVKK209 pKa = 9.87YY210 pKa = 11.28VNGTEE215 pKa = 4.03GMNVEE220 pKa = 4.58VVEE223 pKa = 4.41LPSYY227 pKa = 11.03AGTDD231 pKa = 3.29VTGANVGGNYY241 pKa = 9.74VGNFSDD247 pKa = 3.74EE248 pKa = 4.15ATGKK252 pKa = 10.29VLGNALIAEE261 pKa = 4.61GVDD264 pKa = 3.11ILFVAAGGSGNGVFTAAKK282 pKa = 9.1EE283 pKa = 3.86ADD285 pKa = 3.6GVKK288 pKa = 10.64VIGCDD293 pKa = 2.9VDD295 pKa = 4.4QYY297 pKa = 12.01DD298 pKa = 4.75DD299 pKa = 4.02GANGDD304 pKa = 4.13SNIVLTSGLKK314 pKa = 9.85VMHH317 pKa = 6.38DD318 pKa = 3.26TVYY321 pKa = 11.23NVLNEE326 pKa = 4.2VKK328 pKa = 10.55DD329 pKa = 4.28GSFKK333 pKa = 10.7GANVTLTAADD343 pKa = 4.09DD344 pKa = 3.63ATGFVSEE351 pKa = 4.93EE352 pKa = 4.2GRR354 pKa = 11.84CQLTAEE360 pKa = 4.91AIEE363 pKa = 5.01KK364 pKa = 10.37INAAQALLKK373 pKa = 10.15EE374 pKa = 4.6GKK376 pKa = 9.2IVPAANFNGVTPEE389 pKa = 3.9TFTWW393 pKa = 3.41

Molecular weight: 40.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6F6X0|R6F6X0_9FIRM Cyclic nucleotide-binding domain protein OS=Firmicutes bacterium CAG:65 OX=1262994 GN=BN749_01356 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.36KK9 pKa = 7.93RR10 pKa = 11.84SHH12 pKa = 6.15SKK14 pKa = 8.59VHH16 pKa = 6.03GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2512

0

2512

798008

31

1739

317.7

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.765 ± 0.047

1.498 ± 0.019

5.632 ± 0.04

7.663 ± 0.062

3.965 ± 0.039

7.17 ± 0.04

1.772 ± 0.024

7.106 ± 0.042

6.224 ± 0.043

9.19 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.151 ± 0.027

4.219 ± 0.034

3.321 ± 0.028

3.465 ± 0.031

4.453 ± 0.041

5.508 ± 0.038

5.654 ± 0.035

7.074 ± 0.04

0.926 ± 0.018

4.241 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski