Avian paramyxovirus 4
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5D6F8|G5D6F8_9MONO Matrix protein OS=Avian paramyxovirus 4 OX=28274 GN=M PE=4 SV=1
MM1 pKa = 8.2 DD2 pKa = 4.28 FTDD5 pKa = 3.49 IDD7 pKa = 3.99 AVNSLIEE14 pKa = 4.2 SSSAIIDD21 pKa = 4.24 SIQHH25 pKa = 6.27 GGLQPSGTVGLSQIPKK41 pKa = 9.85 GITSALTKK49 pKa = 10.3 AWEE52 pKa = 4.33 AEE54 pKa = 3.8 AATAGNGDD62 pKa = 4.04 TQHH65 pKa = 6.92 KK66 pKa = 9.11 PDD68 pKa = 4.52 SPEE71 pKa = 3.61 DD72 pKa = 3.57 HH73 pKa = 6.59 QANDD77 pKa = 3.54 TDD79 pKa = 4.21 SPEE82 pKa = 4.12 DD83 pKa = 3.16 TGTNQTIQEE92 pKa = 4.19 ANIVEE97 pKa = 4.59 TPHH100 pKa = 7.17 PEE102 pKa = 3.86 VLSAAKK108 pKa = 10.28 ARR110 pKa = 11.84 LKK112 pKa = 10.52 RR113 pKa = 11.84 PKK115 pKa = 10.13 AGRR118 pKa = 11.84 DD119 pKa = 3.15 THH121 pKa = 7.16 DD122 pKa = 4.34 NPSAQPDD129 pKa = 3.72 HH130 pKa = 6.39 FLKK133 pKa = 10.8 GGPLSPQPAAPWVQSPPIHH152 pKa = 6.48 GGPGTVDD159 pKa = 3.27 PRR161 pKa = 11.84 PSQTQDD167 pKa = 2.49 HH168 pKa = 6.64 SLTGEE173 pKa = 3.94 KK174 pKa = 9.01 WQSSPTKK181 pKa = 10.37 QPEE184 pKa = 4.07 TLNWWNGATRR194 pKa = 11.84 GAPQSEE200 pKa = 4.45 LNQPDD205 pKa = 3.62 STVYY209 pKa = 11.23 ADD211 pKa = 3.76 TAPPSAASACMTTDD225 pKa = 3.51 QVQLLMKK232 pKa = 9.77 EE233 pKa = 4.25 VADD236 pKa = 3.95 MKK238 pKa = 11.11 SLLQALVKK246 pKa = 10.54 NLAVLPQLRR255 pKa = 11.84 NEE257 pKa = 4.05 VAAIRR262 pKa = 11.84 TSQAMIEE269 pKa = 4.22 GTLNSIKK276 pKa = 10.48 ILDD279 pKa = 4.05 PGNYY283 pKa = 9.02 QEE285 pKa = 5.53 SSLNSWFKK293 pKa = 10.31 PRR295 pKa = 11.84 QDD297 pKa = 3.2 HH298 pKa = 5.93 AVVVSGPGNPLTMPTPIQDD317 pKa = 3.28 NTIFLDD323 pKa = 3.7 EE324 pKa = 4.7 LARR327 pKa = 11.84 PHH329 pKa = 6.98 PSLVNPSPPTTNTNVDD345 pKa = 4.43 LGPQKK350 pKa = 10.25 QAAIAYY356 pKa = 9.21 ISAKK360 pKa = 10.48 CKK362 pKa = 10.78 DD363 pKa = 3.41 PGKK366 pKa = 10.12 RR367 pKa = 11.84 DD368 pKa = 3.54 QLSKK372 pKa = 10.72 LIEE375 pKa = 4.23 RR376 pKa = 11.84 ATTLSEE382 pKa = 3.71 INKK385 pKa = 9.41 VKK387 pKa = 10.56 RR388 pKa = 11.84 QALGLL393 pKa = 3.93
Molecular weight: 42.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.316
IPC2_protein 5.219
IPC_protein 5.194
Toseland 5.334
ProMoST 5.423
Dawson 5.296
Bjellqvist 5.385
Wikipedia 5.232
Rodwell 5.232
Grimsley 5.347
Solomon 5.296
Lehninger 5.27
Nozaki 5.474
DTASelect 5.664
Thurlkill 5.397
EMBOSS 5.385
Sillero 5.537
Patrickios 4.457
IPC_peptide 5.308
IPC2_peptide 5.537
IPC2.peptide.svr19 5.522
Protein with the highest isoelectric point:
>tr|G5D6F9|G5D6F9_9MONO Fusion glycoprotein F0 OS=Avian paramyxovirus 4 OX=28274 GN=F PE=3 SV=1
MM1 pKa = 7.74 ADD3 pKa = 3.45 MDD5 pKa = 4.25 TVYY8 pKa = 10.71 INLMADD14 pKa = 3.69 DD15 pKa = 4.42 PTHH18 pKa = 6.18 QKK20 pKa = 10.6 EE21 pKa = 4.43 LLSFPLVPVTGPDD34 pKa = 3.39 GKK36 pKa = 10.78 KK37 pKa = 9.81 EE38 pKa = 3.91 LQHH41 pKa = 5.62 QVRR44 pKa = 11.84 TQSLLASDD52 pKa = 3.93 KK53 pKa = 9.01 QTEE56 pKa = 4.07 RR57 pKa = 11.84 FIFLNTYY64 pKa = 9.8 GFIYY68 pKa = 9.16 DD69 pKa = 3.76 TTPDD73 pKa = 3.29 KK74 pKa = 9.65 TTFSTPEE81 pKa = 4.67 HH82 pKa = 6.29 INQPKK87 pKa = 8.47 RR88 pKa = 11.84 TMVSAAMMTIGLVPANIPLNEE109 pKa = 3.88 LTATVFGLKK118 pKa = 10.05 VRR120 pKa = 11.84 VRR122 pKa = 11.84 KK123 pKa = 8.75 SARR126 pKa = 11.84 YY127 pKa = 9.39 RR128 pKa = 11.84 EE129 pKa = 4.36 VVWYY133 pKa = 9.53 QCNPVPALLAATRR146 pKa = 11.84 FGRR149 pKa = 11.84 QGGLEE154 pKa = 4.13 SSTGVSVKK162 pKa = 10.51 APEE165 pKa = 5.52 KK166 pKa = 10.09 IDD168 pKa = 3.52 CEE170 pKa = 3.95 KK171 pKa = 11.01 DD172 pKa = 3.21 YY173 pKa = 11.09 TYY175 pKa = 11.38 YY176 pKa = 10.37 PYY178 pKa = 10.5 FLSVCYY184 pKa = 10.01 IATSNLFKK192 pKa = 10.5 VPKK195 pKa = 9.4 MVANATNSQLYY206 pKa = 9.02 HH207 pKa = 6.19 LTMQVTFAFPKK218 pKa = 10.26 NIPPANQKK226 pKa = 10.5 LLTQVDD232 pKa = 3.83 EE233 pKa = 4.71 GFEE236 pKa = 4.29 GTVDD240 pKa = 3.43 CHH242 pKa = 6.74 FGNMLKK248 pKa = 10.04 KK249 pKa = 10.15 DD250 pKa = 3.48 RR251 pKa = 11.84 KK252 pKa = 10.08 GNMRR256 pKa = 11.84 TLSQAADD263 pKa = 3.29 KK264 pKa = 10.17 VRR266 pKa = 11.84 RR267 pKa = 11.84 MNILVGIFDD276 pKa = 3.87 LHH278 pKa = 6.63 GPTLFLEE285 pKa = 4.56 YY286 pKa = 9.93 TGKK289 pKa = 8.51 LTKK292 pKa = 10.53 ALLGFMSTSRR302 pKa = 11.84 TAIIPISQLNPMLGQLMWSSDD323 pKa = 3.25 AQIVKK328 pKa = 10.1 LRR330 pKa = 11.84 VVITTSKK337 pKa = 10.43 RR338 pKa = 11.84 GPCGGEE344 pKa = 3.48 QEE346 pKa = 4.66 YY347 pKa = 11.4 VLDD350 pKa = 4.31 PKK352 pKa = 10.51 FTVKK356 pKa = 10.42 KK357 pKa = 9.94 EE358 pKa = 3.9 KK359 pKa = 10.68 ARR361 pKa = 11.84 LNPFKK366 pKa = 10.7 KK367 pKa = 10.21 AAQQ370 pKa = 3.49
Molecular weight: 41.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.842
IPC2_protein 8.814
IPC_protein 8.697
Toseland 9.458
ProMoST 9.224
Dawson 9.736
Bjellqvist 9.458
Wikipedia 9.897
Rodwell 10.072
Grimsley 9.794
Solomon 9.765
Lehninger 9.721
Nozaki 9.589
DTASelect 9.414
Thurlkill 9.575
EMBOSS 9.897
Sillero 9.677
Patrickios 5.054
IPC_peptide 9.75
IPC2_peptide 8.039
IPC2.peptide.svr19 7.889
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4562
370
2211
760.3
84.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.278 ± 0.849
1.797 ± 0.328
5.151 ± 0.235
4.472 ± 0.369
3.31 ± 0.393
5.612 ± 0.352
2.148 ± 0.419
6.094 ± 0.466
4.143 ± 0.616
11.201 ± 0.799
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.302 ± 0.28
4.822 ± 0.35
5.217 ± 0.595
5.129 ± 0.421
5.261 ± 0.353
8.593 ± 0.628
6.664 ± 0.547
6.203 ± 0.399
1.162 ± 0.23
3.42 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here