Avian metaavulavirus 6
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5D6G3|G5D6G3_9MONO Phosphoprotein OS=Avian metaavulavirus 6 OX=2560316 GN=P PE=4 SV=1
MM1 pKa = 7.42 SSVFTDD7 pKa = 3.69 YY8 pKa = 11.8 AKK10 pKa = 11.09 LQDD13 pKa = 4.02 ALVAPSKK20 pKa = 10.52 RR21 pKa = 11.84 KK22 pKa = 9.69 VDD24 pKa = 3.77 SAPSGLLRR32 pKa = 11.84 VGIPVCVLLSEE43 pKa = 4.89 DD44 pKa = 3.83 PEE46 pKa = 4.29 EE47 pKa = 4.06 RR48 pKa = 11.84 WGFVCFCMRR57 pKa = 11.84 WVVSDD62 pKa = 3.69 SATEE66 pKa = 3.88 AMRR69 pKa = 11.84 VGAMLSILSAHH80 pKa = 6.81 ASNMRR85 pKa = 11.84 SHH87 pKa = 6.44 VALAARR93 pKa = 11.84 CGDD96 pKa = 3.3 ADD98 pKa = 3.76 INILEE103 pKa = 4.38 VEE105 pKa = 5.02 AIDD108 pKa = 5.02 HH109 pKa = 5.5 QNQTIRR115 pKa = 11.84 FTGRR119 pKa = 11.84 SNVTDD124 pKa = 3.37 GRR126 pKa = 11.84 ARR128 pKa = 11.84 QMYY131 pKa = 10.38 AIAQDD136 pKa = 4.5 LPPSYY141 pKa = 11.42 NNGSPFVNRR150 pKa = 11.84 DD151 pKa = 2.78 IEE153 pKa = 4.84 DD154 pKa = 3.77 NYY156 pKa = 8.75 PTDD159 pKa = 3.45 MSEE162 pKa = 4.5 LLNMVYY168 pKa = 10.38 SVATQIWVAAMKK180 pKa = 10.93 SMTAPDD186 pKa = 3.71 TSSEE190 pKa = 4.37 SEE192 pKa = 3.84 GRR194 pKa = 11.84 RR195 pKa = 11.84 LAKK198 pKa = 10.21 YY199 pKa = 8.56 IQQNRR204 pKa = 11.84 VIRR207 pKa = 11.84 STILAPATRR216 pKa = 11.84 GEE218 pKa = 4.26 CTRR221 pKa = 11.84 IIRR224 pKa = 11.84 SSLVIRR230 pKa = 11.84 HH231 pKa = 5.71 FLITEE236 pKa = 4.4 IKK238 pKa = 9.89 RR239 pKa = 11.84 ATSMGSNTTRR249 pKa = 11.84 YY250 pKa = 8.83 YY251 pKa = 10.73 ATVGDD256 pKa = 3.58 AAAYY260 pKa = 7.74 FKK262 pKa = 10.82 NAGMAAFFLTLRR274 pKa = 11.84 FGIGTKK280 pKa = 10.51 YY281 pKa = 9.43 STLAVSALSADD292 pKa = 3.73 MKK294 pKa = 10.77 KK295 pKa = 9.96 LQSLIRR301 pKa = 11.84 VYY303 pKa = 10.2 QSKK306 pKa = 11.07 GEE308 pKa = 4.34 DD309 pKa = 3.65 GPYY312 pKa = 9.38 MAFLEE317 pKa = 5.16 DD318 pKa = 3.68 SDD320 pKa = 4.92 LMSFAPGNYY329 pKa = 8.64 PLMYY333 pKa = 9.44 SYY335 pKa = 11.87 AMGVGSILEE344 pKa = 3.99 ASIARR349 pKa = 11.84 YY350 pKa = 9.26 QFARR354 pKa = 11.84 SFMNDD359 pKa = 2.11 TFYY362 pKa = 11.54 RR363 pKa = 11.84 LGVEE367 pKa = 4.17 TAQRR371 pKa = 11.84 NQGSLDD377 pKa = 3.5 EE378 pKa = 4.61 NLAKK382 pKa = 10.28 EE383 pKa = 4.22 LQLSGAEE390 pKa = 3.53 RR391 pKa = 11.84 RR392 pKa = 11.84 AVQEE396 pKa = 4.23 LVTSLDD402 pKa = 3.73 LAGEE406 pKa = 4.17 APVPQRR412 pKa = 11.84 QPTFLNDD419 pKa = 3.12 QEE421 pKa = 5.41 YY422 pKa = 10.85 EE423 pKa = 4.05 DD424 pKa = 5.44 DD425 pKa = 4.08 PPARR429 pKa = 11.84 RR430 pKa = 11.84 QRR432 pKa = 11.84 IEE434 pKa = 4.36 DD435 pKa = 3.89 TPDD438 pKa = 3.91 DD439 pKa = 4.98 DD440 pKa = 5.52 GASQAPPTPGAGLTPYY456 pKa = 10.59 SDD458 pKa = 3.45 NASGLDD464 pKa = 3.3 II465 pKa = 5.27
Molecular weight: 51.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.062
IPC2_protein 5.156
IPC_protein 5.092
Toseland 4.991
ProMoST 5.219
Dawson 5.067
Bjellqvist 5.207
Wikipedia 4.952
Rodwell 4.978
Grimsley 4.902
Solomon 5.067
Lehninger 5.029
Nozaki 5.194
DTASelect 5.372
Thurlkill 4.991
EMBOSS 4.991
Sillero 5.258
Patrickios 4.037
IPC_peptide 5.08
IPC2_peptide 5.245
IPC2.peptide.svr19 5.286
Protein with the highest isoelectric point:
>tr|G5D6G5|G5D6G5_9MONO Fusion glycoprotein F0 OS=Avian metaavulavirus 6 OX=2560316 GN=F PE=3 SV=1
MM1 pKa = 7.19 ATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQPQLRR41 pKa = 11.84 TQYY44 pKa = 11.0 LGDD47 pKa = 3.37 VTGGKK52 pKa = 9.84 KK53 pKa = 9.8 SAIFVNCYY61 pKa = 10.56 GFVEE65 pKa = 4.29 DD66 pKa = 4.99 HH67 pKa = 6.8 GGRR70 pKa = 11.84 DD71 pKa = 3.4 SGFSPISEE79 pKa = 4.15 EE80 pKa = 4.5 SKK82 pKa = 10.92 GSTVTAACITLGSIEE97 pKa = 4.36 YY98 pKa = 10.47 DD99 pKa = 3.18 SDD101 pKa = 3.18 IKK103 pKa = 10.93 EE104 pKa = 4.22 VAKK107 pKa = 10.58 ACYY110 pKa = 9.07 NLQVSVRR117 pKa = 11.84 MSADD121 pKa = 2.95 STQKK125 pKa = 10.21 VVYY128 pKa = 8.98 TINAKK133 pKa = 9.6 PALLFSSRR141 pKa = 11.84 VVRR144 pKa = 11.84 AGGCVVAAEE153 pKa = 4.45 GAIKK157 pKa = 10.41 CPEE160 pKa = 3.99 KK161 pKa = 9.16 MTSDD165 pKa = 3.15 RR166 pKa = 11.84 LYY168 pKa = 9.74 KK169 pKa = 10.02 FRR171 pKa = 11.84 VMFVSLTFLHH181 pKa = 6.75 RR182 pKa = 11.84 SSLFKK187 pKa = 10.81 VSRR190 pKa = 11.84 TVLSMRR196 pKa = 11.84 NSALIAVQAEE206 pKa = 4.41 VKK208 pKa = 10.66 LGFDD212 pKa = 4.54 LPLDD216 pKa = 3.87 HH217 pKa = 7.56 PMAKK221 pKa = 10.07 YY222 pKa = 10.32 LSKK225 pKa = 11.13 EE226 pKa = 4.03 DD227 pKa = 3.58 GQLFATVWVHH237 pKa = 5.99 LCNFKK242 pKa = 9.97 RR243 pKa = 11.84 TDD245 pKa = 3.0 RR246 pKa = 11.84 RR247 pKa = 11.84 GVDD250 pKa = 2.93 RR251 pKa = 11.84 SVEE254 pKa = 4.03 NIRR257 pKa = 11.84 NKK259 pKa = 9.07 VRR261 pKa = 11.84 AMGLKK266 pKa = 8.96 LTLCDD271 pKa = 3.23 LWGPTLVCEE280 pKa = 4.65 ATGKK284 pKa = 8.15 MSKK287 pKa = 10.14 YY288 pKa = 10.12 ALGFFSEE295 pKa = 4.78 TKK297 pKa = 10.42 VGCHH301 pKa = 6.72 PIWKK305 pKa = 9.49 CNSTVAKK312 pKa = 10.45 IMWSCTTWIASAKK325 pKa = 10.37 AIIQASSARR334 pKa = 11.84 ALLTSEE340 pKa = 5.43 DD341 pKa = 3.89 IEE343 pKa = 4.49 AKK345 pKa = 10.52 GAISTDD351 pKa = 3.02 KK352 pKa = 11.09 KK353 pKa = 10.19 KK354 pKa = 9.38 TDD356 pKa = 3.42 GFNPFIKK363 pKa = 9.44 TAKK366 pKa = 9.97
Molecular weight: 36.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.829
IPC2_protein 8.609
IPC_protein 8.463
Toseland 9.268
ProMoST 9.121
Dawson 9.545
Bjellqvist 9.385
Wikipedia 9.648
Rodwell 9.897
Grimsley 9.531
Solomon 9.589
Lehninger 9.56
Nozaki 9.648
DTASelect 9.253
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.589
Patrickios 5.169
IPC_peptide 9.589
IPC2_peptide 8.156
IPC2.peptide.svr19 7.868
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4812
142
2241
687.4
75.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.502 ± 0.869
2.057 ± 0.277
4.738 ± 0.348
5.403 ± 0.464
3.159 ± 0.373
6.068 ± 0.222
2.244 ± 0.459
6.796 ± 0.52
4.468 ± 0.502
10.287 ± 1.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.431 ± 0.242
4.426 ± 0.374
4.468 ± 0.338
4.281 ± 0.455
5.32 ± 0.455
8.084 ± 0.23
6.816 ± 0.494
6.671 ± 0.272
1.164 ± 0.238
2.951 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here