Bat Hp-betacoronavirus/Zhejiang2013
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A088DI26|A0A088DI26_9BETC Nucleoprotein OS=Bat Hp-betacoronavirus/Zhejiang2013 OX=1541205 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.19 FFSLYY7 pKa = 10.27 TFGSFHH13 pKa = 5.26 MHH15 pKa = 6.13 AAKK18 pKa = 10.22 LASLLSNSTEE28 pKa = 3.95 HH29 pKa = 7.12 VEE31 pKa = 4.89 QAVQHH36 pKa = 6.5 LNTKK40 pKa = 8.64 LTADD44 pKa = 2.95 IMLCIFLVIYY54 pKa = 7.4 LICYY58 pKa = 8.01 FLRR61 pKa = 11.84 TDD63 pKa = 3.21 SFIAVVFKK71 pKa = 10.85 YY72 pKa = 10.42 LAGLLTGGFLCLGLFLDD89 pKa = 4.96 TPTLLLKK96 pKa = 10.03 ATIGVVLFMFSLGFICRR113 pKa = 11.84 ITLAIRR119 pKa = 11.84 CKK121 pKa = 10.76 SLVPLCADD129 pKa = 3.85 DD130 pKa = 5.34 DD131 pKa = 4.58 CFVNYY136 pKa = 10.07 NAGGKK141 pKa = 6.52 TYY143 pKa = 10.82 CMPFDD148 pKa = 4.5 PNEE151 pKa = 4.25 PYY153 pKa = 9.55 LTLVVHH159 pKa = 6.4 QNGITCGSYY168 pKa = 10.85 KK169 pKa = 10.7 LYY171 pKa = 10.91 GDD173 pKa = 3.43 VSIADD178 pKa = 4.61 RR179 pKa = 11.84 IYY181 pKa = 11.13 LVTLTKK187 pKa = 10.32 SVPYY191 pKa = 9.98 SLQNIFDD198 pKa = 4.6 AEE200 pKa = 4.3 LCTIAFYY207 pKa = 10.48 IADD210 pKa = 3.74 CAVIEE215 pKa = 4.25 DD216 pKa = 4.23 HH217 pKa = 5.85 TTAGKK222 pKa = 8.25 TPRR225 pKa = 11.84 LEE227 pKa = 4.47 LKK229 pKa = 10.12 SDD231 pKa = 4.79 PIYY234 pKa = 10.19 EE235 pKa = 4.47 VPCATIDD242 pKa = 3.58 VPLL245 pKa = 5.05
Molecular weight: 27.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.21
IPC2_protein 5.105
IPC_protein 5.041
Toseland 5.067
ProMoST 5.27
Dawson 5.13
Bjellqvist 5.232
Wikipedia 5.08
Rodwell 5.041
Grimsley 5.029
Solomon 5.13
Lehninger 5.105
Nozaki 5.296
DTASelect 5.512
Thurlkill 5.169
EMBOSS 5.194
Sillero 5.359
Patrickios 0.388
IPC_peptide 5.143
IPC2_peptide 5.347
IPC2.peptide.svr19 5.177
Protein with the highest isoelectric point:
>tr|A0A088DIE1|A0A088DIE1_9BETC 2'-O-methyltransferase OS=Bat Hp-betacoronavirus/Zhejiang2013 OX=1541205 PE=3 SV=1
MM1 pKa = 7.31 AAEE4 pKa = 4.32 GQRR7 pKa = 11.84 VTFLTQGSNQNNNNQEE23 pKa = 4.12 NQDD26 pKa = 3.44 GRR28 pKa = 11.84 GARR31 pKa = 11.84 PKK33 pKa = 9.17 TRR35 pKa = 11.84 KK36 pKa = 8.95 PRR38 pKa = 11.84 PAPQNNVSWFTPLTQHH54 pKa = 6.68 GKK56 pKa = 9.18 QALTFNPGQGVPLNANDD73 pKa = 5.2 DD74 pKa = 3.75 PDD76 pKa = 4.07 SRR78 pKa = 11.84 IGYY81 pKa = 5.38 WRR83 pKa = 11.84 RR84 pKa = 11.84 NIRR87 pKa = 11.84 TVTKK91 pKa = 10.3 NGKK94 pKa = 6.92 PQKK97 pKa = 10.34 LDD99 pKa = 3.39 PRR101 pKa = 11.84 WYY103 pKa = 10.04 FYY105 pKa = 11.53 YY106 pKa = 10.65 LGTGPEE112 pKa = 3.91 QNLKK116 pKa = 10.49 YY117 pKa = 10.28 LQQKK121 pKa = 10.41 DD122 pKa = 3.88 GIVWVAAHH130 pKa = 6.71 GALNVPVKK138 pKa = 10.02 TVGTRR143 pKa = 11.84 NPANDD148 pKa = 3.52 QAIVAAFAPGTEE160 pKa = 4.22 LPKK163 pKa = 10.83 GFYY166 pKa = 10.61 VEE168 pKa = 5.11 GSRR171 pKa = 11.84 STSSASSAASSRR183 pKa = 11.84 SNSRR187 pKa = 11.84 SRR189 pKa = 11.84 NSSLSRR195 pKa = 11.84 SSSPGRR201 pKa = 11.84 GLPPATDD208 pKa = 3.94 PNGVLAALLLTKK220 pKa = 10.73 LEE222 pKa = 4.29 ALDD225 pKa = 4.4 AKK227 pKa = 11.27 VNGPKK232 pKa = 9.84 QPPVVTKK239 pKa = 9.39 KK240 pKa = 8.76 TAAEE244 pKa = 3.84 IAAKK248 pKa = 9.83 PRR250 pKa = 11.84 QKK252 pKa = 10.25 RR253 pKa = 11.84 VAHH256 pKa = 6.45 KK257 pKa = 10.33 GYY259 pKa = 10.37 NVNAAYY265 pKa = 10.1 GRR267 pKa = 11.84 RR268 pKa = 11.84 GPGPYY273 pKa = 9.33 QGNFGTQEE281 pKa = 3.91 FNKK284 pKa = 10.55 LGTDD288 pKa = 3.65 YY289 pKa = 11.06 PKK291 pKa = 10.28 WPQIAQLAPTPSAFFGMSRR310 pKa = 11.84 FAVQKK315 pKa = 10.75 NDD317 pKa = 3.33 DD318 pKa = 4.31 GTWLTYY324 pKa = 10.12 HH325 pKa = 6.3 GHH327 pKa = 6.05 IKK329 pKa = 9.7 MDD331 pKa = 3.35 EE332 pKa = 4.13 SDD334 pKa = 4.11 PNFQVWMTEE343 pKa = 3.81 LQQNIDD349 pKa = 3.2 AYY351 pKa = 10.32 KK352 pKa = 10.5 NFPQKK357 pKa = 10.37 EE358 pKa = 4.35 EE359 pKa = 4.06 KK360 pKa = 10.17 KK361 pKa = 10.23 SRR363 pKa = 11.84 KK364 pKa = 9.22 PKK366 pKa = 9.07 TKK368 pKa = 10.19 NVDD371 pKa = 3.33 MAPQDD376 pKa = 3.68 VMGAAAVDD384 pKa = 4.3 LEE386 pKa = 4.49 WDD388 pKa = 3.62 SSIDD392 pKa = 3.35 QTGPNTIVVKK402 pKa = 10.14 PKK404 pKa = 10.15 KK405 pKa = 8.97 QRR407 pKa = 11.84 KK408 pKa = 7.3 PAADD412 pKa = 3.59 NNISEE417 pKa = 4.39 II418 pKa = 3.96
Molecular weight: 45.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.076
IPC2_protein 9.341
IPC_protein 9.297
Toseland 10.16
ProMoST 9.75
Dawson 10.321
Bjellqvist 9.94
Wikipedia 10.452
Rodwell 10.862
Grimsley 10.365
Solomon 10.335
Lehninger 10.306
Nozaki 10.131
DTASelect 9.94
Thurlkill 10.175
EMBOSS 10.555
Sillero 10.218
Patrickios 10.526
IPC_peptide 10.335
IPC2_peptide 8.331
IPC2.peptide.svr19 8.411
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
10159
72
7247
1128.8
126.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.097 ± 0.29
3.406 ± 0.444
5.375 ± 0.609
4.233 ± 0.376
4.922 ± 0.318
5.571 ± 0.288
2.136 ± 0.237
4.991 ± 0.409
5.512 ± 0.758
8.406 ± 0.588
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.229
5.473 ± 0.628
4.213 ± 0.606
3.642 ± 0.686
3.691 ± 0.323
6.566 ± 0.298
7.668 ± 0.951
8.859 ± 0.748
1.073 ± 0.165
4.676 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here