bacterium HR28

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5Z228|A0A2H5Z228_9BACT Stage II sporulation protein E OS=bacterium HR28 OX=2035423 GN=spoIIE PE=4 SV=1
MM1 pKa = 7.65GKK3 pKa = 9.73RR4 pKa = 11.84LVHH7 pKa = 6.62LLLAVAMAVSAFAGLTIAPAGAAQEE32 pKa = 4.54VPSGTIYY39 pKa = 10.53FPWVPNGEE47 pKa = 4.5EE48 pKa = 4.07YY49 pKa = 11.11AGMGPWYY56 pKa = 10.64SSVTIQNFTEE66 pKa = 4.7FPVRR70 pKa = 11.84ANIYY74 pKa = 9.88KK75 pKa = 10.32ADD77 pKa = 3.88GTKK80 pKa = 9.16LTSVTFEE87 pKa = 3.75PWASKK92 pKa = 10.03SWSSADD98 pKa = 3.44LFGNGSGAGVFVDD111 pKa = 4.14AAVGPLTVTRR121 pKa = 11.84GDD123 pKa = 3.33TDD125 pKa = 3.23IDD127 pKa = 4.01SVSVGRR133 pKa = 11.84CVVQSVSISQGATVFVQGVDD153 pKa = 4.07YY154 pKa = 10.57IWSQTGSRR162 pKa = 11.84LDD164 pKa = 3.45ITWIGSAPAAGTDD177 pKa = 3.38YY178 pKa = 10.37TVNVACDD185 pKa = 3.59GLLGGVVKK193 pKa = 10.18MAAPQALISAAWTGTGHH210 pKa = 6.67EE211 pKa = 4.7SVTGYY216 pKa = 9.7TSLPVEE222 pKa = 5.47DD223 pKa = 3.62VWTWPRR229 pKa = 11.84SWVFPIIQTNNGWNSVMHH247 pKa = 6.06ITNFGGGNCSVNVDD261 pKa = 4.19LYY263 pKa = 9.52QTPTRR268 pKa = 11.84AGAPGASGPSEE279 pKa = 3.82GHH281 pKa = 5.87FSEE284 pKa = 5.43LLAAGEE290 pKa = 4.29TWHH293 pKa = 7.12IDD295 pKa = 3.31LVQDD299 pKa = 3.96YY300 pKa = 9.21GWPADD305 pKa = 3.9WIGTAFITADD315 pKa = 3.69CGVAASVDD323 pKa = 3.72RR324 pKa = 11.84LKK326 pKa = 10.9AAQPWGDD333 pKa = 4.01PVNMAVTNVAQPTFGNWSEE352 pKa = 4.41VYY354 pKa = 10.7APLVYY359 pKa = 10.39QNYY362 pKa = 9.64NGWNTGISIVNLDD375 pKa = 3.64PDD377 pKa = 4.31FNNNVQVQFYY387 pKa = 10.3NRR389 pKa = 11.84DD390 pKa = 3.13GTLVHH395 pKa = 6.42SEE397 pKa = 4.3SLTIPPRR404 pKa = 11.84AMEE407 pKa = 4.09WLYY410 pKa = 11.24LPEE413 pKa = 4.23RR414 pKa = 11.84TDD416 pKa = 4.49LGTQGLSQAVIRR428 pKa = 11.84SLSGLNLAAAVDD440 pKa = 4.19SVKK443 pKa = 9.9YY444 pKa = 9.41TGIDD448 pKa = 3.17QDD450 pKa = 3.74VGQATSYY457 pKa = 10.49LAQQGTRR464 pKa = 11.84AARR467 pKa = 11.84PGEE470 pKa = 4.2GAQNGLLSVALFQKK484 pKa = 10.47QFGLTAQQDD493 pKa = 3.84NSGIAYY499 pKa = 9.71FNANTGAPATVEE511 pKa = 3.87VTFYY515 pKa = 10.92DD516 pKa = 3.85GAGFRR521 pKa = 11.84VAPTLVNDD529 pKa = 4.55LVINLPPAGGGILYY543 pKa = 10.01APWYY547 pKa = 10.7GEE549 pKa = 3.89MPFGFRR555 pKa = 11.84GSVVLEE561 pKa = 4.17VTQGGPVACVSNNVNYY577 pKa = 9.74QVQFDD582 pKa = 3.75GTATYY587 pKa = 11.19NCFIRR592 pKa = 11.84EE593 pKa = 4.25AVDD596 pKa = 3.57GAVPRR601 pKa = 11.84PEE603 pKa = 3.76WTLEE607 pKa = 3.72ARR609 pKa = 11.84PANAANLAARR619 pKa = 11.84TVDD622 pKa = 3.13TDD624 pKa = 3.49VEE626 pKa = 4.51RR627 pKa = 11.84FQHH630 pKa = 5.21TVTAVVRR637 pKa = 11.84FAGQPQTGVPVLFEE651 pKa = 4.08ILNGSRR657 pKa = 11.84NDD659 pKa = 4.06DD660 pKa = 3.66PAEE663 pKa = 4.18NDD665 pKa = 3.04QGTYY669 pKa = 10.72NPAGDD674 pKa = 4.46DD675 pKa = 3.4DD676 pKa = 5.03VVVNTNNNGVASWTYY691 pKa = 10.87QDD693 pKa = 3.96GGAANTSGVDD703 pKa = 3.83TILVCADD710 pKa = 3.17VNGNSQCDD718 pKa = 3.5PGEE721 pKa = 3.94PRR723 pKa = 11.84QTVRR727 pKa = 11.84KK728 pKa = 8.17VWATATVDD736 pKa = 3.6QGQPPQDD743 pKa = 3.33AGVVGDD749 pKa = 3.81NTEE752 pKa = 4.23DD753 pKa = 3.26LVLAIDD759 pKa = 4.42VDD761 pKa = 4.3PDD763 pKa = 3.34QLNAGLPLVLRR774 pKa = 11.84IVNEE778 pKa = 4.02TGGDD782 pKa = 3.45VSFAVCNQPDD792 pKa = 3.91RR793 pKa = 11.84NVQPWFVSADD803 pKa = 3.76TNVPLYY809 pKa = 10.3ICADD813 pKa = 3.79PAPAGGTFQVEE824 pKa = 4.7AYY826 pKa = 9.36WDD828 pKa = 3.75VNANGQVDD836 pKa = 3.77AGVDD840 pKa = 3.63VPLIGSPVNFTWW852 pKa = 3.79

Molecular weight: 90.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5Z8G3|A0A2H5Z8G3_9BACT Cyclic nucleotide-binding domain-containing protein OS=bacterium HR28 OX=2035423 GN=HRbin28_02463 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 9.86KK4 pKa = 10.39AKK6 pKa = 9.54ADD8 pKa = 4.49RR9 pKa = 11.84IIITLEE15 pKa = 3.78CTVCRR20 pKa = 11.84EE21 pKa = 4.05RR22 pKa = 11.84NYY24 pKa = 9.2VTQKK28 pKa = 10.25NRR30 pKa = 11.84RR31 pKa = 11.84NDD33 pKa = 3.56PGRR36 pKa = 11.84LEE38 pKa = 3.88LRR40 pKa = 11.84KK41 pKa = 9.79YY42 pKa = 10.31CPRR45 pKa = 11.84CRR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.32QVHH52 pKa = 6.67RR53 pKa = 11.84EE54 pKa = 3.63TRR56 pKa = 3.29

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2802

0

2802

898898

29

2214

320.8

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.497 ± 0.056

0.822 ± 0.016

4.607 ± 0.026

6.764 ± 0.052

3.267 ± 0.027

7.986 ± 0.048

2.074 ± 0.021

4.624 ± 0.032

1.624 ± 0.026

11.854 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.017

1.81 ± 0.02

6.156 ± 0.038

3.578 ± 0.031

8.931 ± 0.048

4.541 ± 0.035

5.469 ± 0.036

8.641 ± 0.045

1.726 ± 0.023

2.383 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski