Amino acid dipepetide frequency for Clostridiaceae bacterium 14S0207

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.023AlaAla: 3.023 ± 0.087
0.672AlaCys: 0.672 ± 0.034
2.184AlaAsp: 2.184 ± 0.057
3.049AlaGlu: 3.049 ± 0.077
2.237AlaPhe: 2.237 ± 0.055
3.151AlaGly: 3.151 ± 0.069
0.726AlaHis: 0.726 ± 0.03
5.421AlaIle: 5.421 ± 0.086
4.518AlaLys: 4.518 ± 0.09
5.265AlaLeu: 5.265 ± 0.095
1.452AlaMet: 1.452 ± 0.044
2.484AlaAsn: 2.484 ± 0.068
1.274AlaPro: 1.274 ± 0.043
1.37AlaGln: 1.37 ± 0.044
1.674AlaArg: 1.674 ± 0.052
2.869AlaSer: 2.869 ± 0.062
2.608AlaThr: 2.608 ± 0.07
3.168AlaVal: 3.168 ± 0.078
0.337AlaTrp: 0.337 ± 0.021
2.145AlaTyr: 2.145 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.593CysAla: 0.593 ± 0.029
0.288CysCys: 0.288 ± 0.027
0.665CysAsp: 0.665 ± 0.028
0.853CysGlu: 0.853 ± 0.034
0.601CysPhe: 0.601 ± 0.027
1.056CysGly: 1.056 ± 0.046
0.182CysHis: 0.182 ± 0.015
1.373CysIle: 1.373 ± 0.042
1.315CysLys: 1.315 ± 0.038
0.997CysLeu: 0.997 ± 0.032
0.333CysMet: 0.333 ± 0.02
0.918CysAsn: 0.918 ± 0.039
0.477CysPro: 0.477 ± 0.025
0.201CysGln: 0.201 ± 0.015
0.415CysArg: 0.415 ± 0.023
0.88CysSer: 0.88 ± 0.032
0.658CysThr: 0.658 ± 0.028
0.706CysVal: 0.706 ± 0.027
0.072CysTrp: 0.072 ± 0.01
0.523CysTyr: 0.523 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
2.249AspAla: 2.249 ± 0.06
0.595AspCys: 0.595 ± 0.028
2.251AspAsp: 2.251 ± 0.061
4.156AspGlu: 4.156 ± 0.086
2.65AspPhe: 2.65 ± 0.061
2.852AspGly: 2.852 ± 0.072
0.551AspHis: 0.551 ± 0.024
5.97AspIle: 5.97 ± 0.094
5.843AspLys: 5.843 ± 0.1
4.707AspLeu: 4.707 ± 0.082
1.351AspMet: 1.351 ± 0.046
3.244AspAsn: 3.244 ± 0.072
1.319AspPro: 1.319 ± 0.041
0.91AspGln: 0.91 ± 0.032
1.649AspArg: 1.649 ± 0.051
2.82AspSer: 2.82 ± 0.061
2.46AspThr: 2.46 ± 0.056
3.443AspVal: 3.443 ± 0.072
0.302AspTrp: 0.302 ± 0.02
2.214AspTyr: 2.214 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
3.582GluAla: 3.582 ± 0.078
0.771GluCys: 0.771 ± 0.032
4.139GluAsp: 4.139 ± 0.082
7.439GluGlu: 7.439 ± 0.144
3.21GluPhe: 3.21 ± 0.061
4.237GluGly: 4.237 ± 0.085
0.956GluHis: 0.956 ± 0.036
7.202GluIle: 7.202 ± 0.094
8.295GluLys: 8.295 ± 0.137
6.989GluLeu: 6.989 ± 0.117
1.791GluMet: 1.791 ± 0.042
5.855GluAsn: 5.855 ± 0.097
1.386GluPro: 1.386 ± 0.039
2.166GluGln: 2.166 ± 0.051
2.373GluArg: 2.373 ± 0.06
3.556GluSer: 3.556 ± 0.072
2.832GluThr: 2.832 ± 0.059
4.815GluVal: 4.815 ± 0.084
0.365GluTrp: 0.365 ± 0.022
3.021GluTyr: 3.021 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.037PheAla: 2.037 ± 0.051
0.65PheCys: 0.65 ± 0.026
2.124PheAsp: 2.124 ± 0.049
2.513PheGlu: 2.513 ± 0.066
2.091PhePhe: 2.091 ± 0.062
2.564PheGly: 2.564 ± 0.066
0.58PheHis: 0.58 ± 0.029
5.081PheIle: 5.081 ± 0.106
4.496PheLys: 4.496 ± 0.075
4.312PheLeu: 4.312 ± 0.09
1.253PheMet: 1.253 ± 0.043
3.355PheAsn: 3.355 ± 0.076
1.127PhePro: 1.127 ± 0.038
1.057PheGln: 1.057 ± 0.035
1.217PheArg: 1.217 ± 0.044
3.354PheSer: 3.354 ± 0.069
2.4PheThr: 2.4 ± 0.055
2.476PheVal: 2.476 ± 0.056
0.301PheTrp: 0.301 ± 0.018
1.915PheTyr: 1.915 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
3.669GlyAla: 3.669 ± 0.081
0.907GlyCys: 0.907 ± 0.037
2.949GlyAsp: 2.949 ± 0.067
4.307GlyGlu: 4.307 ± 0.075
2.906GlyPhe: 2.906 ± 0.068
3.908GlyGly: 3.908 ± 0.1
0.91GlyHis: 0.91 ± 0.038
6.789GlyIle: 6.789 ± 0.114
5.743GlyLys: 5.743 ± 0.082
5.03GlyLeu: 5.03 ± 0.095
1.722GlyMet: 1.722 ± 0.05
3.17GlyAsn: 3.17 ± 0.066
1.215GlyPro: 1.215 ± 0.038
1.48GlyGln: 1.48 ± 0.049
2.039GlyArg: 2.039 ± 0.057
3.265GlySer: 3.265 ± 0.07
3.373GlyThr: 3.373 ± 0.086
4.34GlyVal: 4.34 ± 0.087
0.438GlyTrp: 0.438 ± 0.026
2.721GlyTyr: 2.721 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
0.592HisAla: 0.592 ± 0.024
0.201HisCys: 0.201 ± 0.017
0.655HisAsp: 0.655 ± 0.028
0.844HisGlu: 0.844 ± 0.026
0.565HisPhe: 0.565 ± 0.027
0.895HisGly: 0.895 ± 0.034
0.242HisHis: 0.242 ± 0.017
1.356HisIle: 1.356 ± 0.042
1.3HisLys: 1.3 ± 0.044
1.092HisLeu: 1.092 ± 0.037
0.363HisMet: 0.363 ± 0.021
0.889HisAsn: 0.889 ± 0.034
0.553HisPro: 0.553 ± 0.025
0.268HisGln: 0.268 ± 0.018
0.53HisArg: 0.53 ± 0.028
0.781HisSer: 0.781 ± 0.03
0.671HisThr: 0.671 ± 0.029
0.795HisVal: 0.795 ± 0.03
0.105HisTrp: 0.105 ± 0.01
0.517HisTyr: 0.517 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.252IleAla: 5.252 ± 0.093
1.453IleCys: 1.453 ± 0.044
5.545IleAsp: 5.545 ± 0.096
7.412IleGlu: 7.412 ± 0.11
4.697IlePhe: 4.697 ± 0.109
6.044IleGly: 6.044 ± 0.109
1.296IleHis: 1.296 ± 0.04
9.971IleIle: 9.971 ± 0.158
9.997IleLys: 9.997 ± 0.11
10.244IleLeu: 10.244 ± 0.159
2.292IleMet: 2.292 ± 0.054
6.773IleAsn: 6.773 ± 0.119
3.433IlePro: 3.433 ± 0.078
2.487IleGln: 2.487 ± 0.055
2.964IleArg: 2.964 ± 0.06
7.081IleSer: 7.081 ± 0.112
4.999IleThr: 4.999 ± 0.085
5.957IleVal: 5.957 ± 0.097
0.595IleTrp: 0.595 ± 0.028
3.812IleTyr: 3.812 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
4.717LysAla: 4.717 ± 0.096
1.086LysCys: 1.086 ± 0.042
6.535LysAsp: 6.535 ± 0.111
10.144LysGlu: 10.144 ± 0.164
3.883LysPhe: 3.883 ± 0.069
5.671LysGly: 5.671 ± 0.079
1.219LysHis: 1.219 ± 0.041
9.13LysIle: 9.13 ± 0.111
9.783LysLys: 9.783 ± 0.158
8.426LysLeu: 8.426 ± 0.11
2.524LysMet: 2.524 ± 0.053
7.745LysAsn: 7.745 ± 0.116
2.165LysPro: 2.165 ± 0.066
2.819LysGln: 2.819 ± 0.066
3.166LysArg: 3.166 ± 0.06
5.428LysSer: 5.428 ± 0.083
4.419LysThr: 4.419 ± 0.079
6.668LysVal: 6.668 ± 0.1
0.623LysTrp: 0.623 ± 0.029
4.444LysTyr: 4.444 ± 0.083
0.0LysXaa: 0.0 ± 0.0
Leu
4.669LeuAla: 4.669 ± 0.092
1.155LeuCys: 1.155 ± 0.042
5.099LeuAsp: 5.099 ± 0.091
6.425LeuGlu: 6.425 ± 0.097
3.613LeuPhe: 3.613 ± 0.081
6.342LeuGly: 6.342 ± 0.102
1.075LeuHis: 1.075 ± 0.034
8.872LeuIle: 8.872 ± 0.134
9.867LeuLys: 9.867 ± 0.134
7.884LeuLeu: 7.884 ± 0.126
2.222LeuMet: 2.222 ± 0.055
6.746LeuAsn: 6.746 ± 0.1
2.601LeuPro: 2.601 ± 0.058
2.541LeuGln: 2.541 ± 0.064
3.062LeuArg: 3.062 ± 0.06
6.551LeuSer: 6.551 ± 0.1
4.505LeuThr: 4.505 ± 0.081
5.2LeuVal: 5.2 ± 0.084
0.54LeuTrp: 0.54 ± 0.025
3.369LeuTyr: 3.369 ± 0.063
0.0LeuXaa: 0.0 ± 0.0
Met
1.569MetAla: 1.569 ± 0.044
0.308MetCys: 0.308 ± 0.02
1.485MetAsp: 1.485 ± 0.037
2.023MetGlu: 2.023 ± 0.047
1.062MetPhe: 1.062 ± 0.036
1.679MetGly: 1.679 ± 0.047
0.348MetHis: 0.348 ± 0.021
2.128MetIle: 2.128 ± 0.053
2.579MetLys: 2.579 ± 0.05
2.202MetLeu: 2.202 ± 0.059
0.627MetMet: 0.627 ± 0.028
1.718MetAsn: 1.718 ± 0.047
0.841MetPro: 0.841 ± 0.034
0.709MetGln: 0.709 ± 0.029
0.82MetArg: 0.82 ± 0.028
1.568MetSer: 1.568 ± 0.048
1.037MetThr: 1.037 ± 0.036
1.504MetVal: 1.504 ± 0.047
0.141MetTrp: 0.141 ± 0.012
0.938MetTyr: 0.938 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.693AsnAla: 2.693 ± 0.06
0.897AsnCys: 0.897 ± 0.038
2.86AsnAsp: 2.86 ± 0.075
4.467AsnGlu: 4.467 ± 0.088
3.032AsnPhe: 3.032 ± 0.069
3.563AsnGly: 3.563 ± 0.07
0.783AsnHis: 0.783 ± 0.032
7.945AsnIle: 7.945 ± 0.129
7.766AsnLys: 7.766 ± 0.12
6.442AsnLeu: 6.442 ± 0.1
1.698AsnMet: 1.698 ± 0.05
5.209AsnAsn: 5.209 ± 0.119
2.128AsnPro: 2.128 ± 0.046
1.44AsnGln: 1.44 ± 0.05
1.994AsnArg: 1.994 ± 0.048
4.12AsnSer: 4.12 ± 0.088
3.106AsnThr: 3.106 ± 0.071
3.984AsnVal: 3.984 ± 0.086
0.46AsnTrp: 0.46 ± 0.026
2.805AsnTyr: 2.805 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
1.191ProAla: 1.191 ± 0.038
0.365ProCys: 0.365 ± 0.024
1.175ProAsp: 1.175 ± 0.039
1.986ProGlu: 1.986 ± 0.063
1.303ProPhe: 1.303 ± 0.044
1.496ProGly: 1.496 ± 0.051
0.45ProHis: 0.45 ± 0.022
2.827ProIle: 2.827 ± 0.067
2.627ProLys: 2.627 ± 0.057
2.366ProLeu: 2.366 ± 0.064
0.687ProMet: 0.687 ± 0.026
1.662ProAsn: 1.662 ± 0.058
0.554ProPro: 0.554 ± 0.026
0.817ProGln: 0.817 ± 0.033
0.762ProArg: 0.762 ± 0.028
1.782ProSer: 1.782 ± 0.047
1.56ProThr: 1.56 ± 0.05
1.924ProVal: 1.924 ± 0.049
0.189ProTrp: 0.189 ± 0.015
1.286ProTyr: 1.286 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
1.249GlnAla: 1.249 ± 0.048
0.377GlnCys: 0.377 ± 0.02
1.291GlnAsp: 1.291 ± 0.032
1.978GlnGlu: 1.978 ± 0.061
1.026GlnPhe: 1.026 ± 0.032
1.65GlnGly: 1.65 ± 0.051
0.37GlnHis: 0.37 ± 0.021
2.176GlnIle: 2.176 ± 0.054
2.372GlnLys: 2.372 ± 0.062
2.317GlnLeu: 2.317 ± 0.05
0.582GlnMet: 0.582 ± 0.025
1.785GlnAsn: 1.785 ± 0.049
0.579GlnPro: 0.579 ± 0.026
0.778GlnGln: 0.778 ± 0.039
0.992GlnArg: 0.992 ± 0.041
1.366GlnSer: 1.366 ± 0.044
1.022GlnThr: 1.022 ± 0.037
1.662GlnVal: 1.662 ± 0.05
0.273GlnTrp: 0.273 ± 0.018
1.211GlnTyr: 1.211 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
1.624ArgAla: 1.624 ± 0.046
0.436ArgCys: 0.436 ± 0.024
1.666ArgAsp: 1.666 ± 0.05
2.864ArgGlu: 2.864 ± 0.067
1.361ArgPhe: 1.361 ± 0.046
1.874ArgGly: 1.874 ± 0.048
0.433ArgHis: 0.433 ± 0.022
3.127ArgIle: 3.127 ± 0.062
3.137ArgLys: 3.137 ± 0.067
2.788ArgLeu: 2.788 ± 0.061
0.859ArgMet: 0.859 ± 0.03
1.972ArgAsn: 1.972 ± 0.049
0.804ArgPro: 0.804 ± 0.032
0.862ArgGln: 0.862 ± 0.033
1.308ArgArg: 1.308 ± 0.046
1.505ArgSer: 1.505 ± 0.045
1.49ArgThr: 1.49 ± 0.043
2.084ArgVal: 2.084 ± 0.051
0.207ArgTrp: 0.207 ± 0.016
1.348ArgTyr: 1.348 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
2.834SerAla: 2.834 ± 0.055
0.768SerCys: 0.768 ± 0.032
2.691SerAsp: 2.691 ± 0.052
3.706SerGlu: 3.706 ± 0.069
2.998SerPhe: 2.998 ± 0.061
3.753SerGly: 3.753 ± 0.077
0.89SerHis: 0.89 ± 0.033
6.819SerIle: 6.819 ± 0.108
6.232SerLys: 6.232 ± 0.093
6.15SerLeu: 6.15 ± 0.087
1.664SerMet: 1.664 ± 0.051
3.924SerAsn: 3.924 ± 0.089
1.583SerPro: 1.583 ± 0.043
1.504SerGln: 1.504 ± 0.045
1.901SerArg: 1.901 ± 0.051
4.11SerSer: 4.11 ± 0.083
3.186SerThr: 3.186 ± 0.071
3.628SerVal: 3.628 ± 0.067
0.424SerTrp: 0.424 ± 0.023
2.513SerTyr: 2.513 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
2.587ThrAla: 2.587 ± 0.064
0.611ThrCys: 0.611 ± 0.028
2.117ThrAsp: 2.117 ± 0.055
3.1ThrGlu: 3.1 ± 0.063
2.31ThrPhe: 2.31 ± 0.055
3.292ThrGly: 3.292 ± 0.07
0.771ThrHis: 0.771 ± 0.029
5.067ThrIle: 5.067 ± 0.095
4.041ThrLys: 4.041 ± 0.079
5.002ThrLeu: 5.002 ± 0.073
1.137ThrMet: 1.137 ± 0.037
2.775ThrAsn: 2.775 ± 0.065
1.766ThrPro: 1.766 ± 0.048
1.152ThrGln: 1.152 ± 0.039
1.49ThrArg: 1.49 ± 0.05
3.126ThrSer: 3.126 ± 0.073
2.597ThrThr: 2.597 ± 0.068
3.254ThrVal: 3.254 ± 0.073
0.38ThrTrp: 0.38 ± 0.022
2.046ThrTyr: 2.046 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
3.331ValAla: 3.331 ± 0.08
0.915ValCys: 0.915 ± 0.035
3.468ValAsp: 3.468 ± 0.072
4.392ValGlu: 4.392 ± 0.08
2.889ValPhe: 2.889 ± 0.066
4.0ValGly: 4.0 ± 0.083
0.844ValHis: 0.844 ± 0.03
6.115ValIle: 6.115 ± 0.094
5.91ValLys: 5.91 ± 0.097
5.9ValLeu: 5.9 ± 0.082
1.527ValMet: 1.527 ± 0.044
3.711ValAsn: 3.711 ± 0.069
1.86ValPro: 1.86 ± 0.047
1.671ValGln: 1.671 ± 0.047
1.825ValArg: 1.825 ± 0.05
4.0ValSer: 4.0 ± 0.068
3.322ValThr: 3.322 ± 0.074
4.198ValVal: 4.198 ± 0.083
0.365ValTrp: 0.365 ± 0.021
2.286ValTyr: 2.286 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
0.348TrpAla: 0.348 ± 0.022
0.109TrpCys: 0.109 ± 0.013
0.382TrpAsp: 0.382 ± 0.024
0.42TrpGlu: 0.42 ± 0.024
0.31TrpPhe: 0.31 ± 0.021
0.44TrpGly: 0.44 ± 0.023
0.11TrpHis: 0.11 ± 0.011
0.679TrpIle: 0.679 ± 0.029
0.553TrpLys: 0.553 ± 0.026
0.509TrpLeu: 0.509 ± 0.025
0.155TrpMet: 0.155 ± 0.015
0.459TrpAsn: 0.459 ± 0.026
0.134TrpPro: 0.134 ± 0.014
0.186TrpGln: 0.186 ± 0.015
0.222TrpArg: 0.222 ± 0.015
0.42TrpSer: 0.42 ± 0.026
0.307TrpThr: 0.307 ± 0.022
0.369TrpVal: 0.369 ± 0.017
0.065TrpTrp: 0.065 ± 0.009
0.293TrpTyr: 0.293 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.819TyrAla: 1.819 ± 0.05
0.541TyrCys: 0.541 ± 0.027
2.256TyrAsp: 2.256 ± 0.056
2.902TyrGlu: 2.902 ± 0.065
2.16TyrPhe: 2.16 ± 0.054
2.414TyrGly: 2.414 ± 0.059
0.476TyrHis: 0.476 ± 0.026
4.256TyrIle: 4.256 ± 0.083
4.219TyrLys: 4.219 ± 0.079
3.715TyrLeu: 3.715 ± 0.075
1.049TyrMet: 1.049 ± 0.034
2.999TyrAsn: 2.999 ± 0.076
1.211TyrPro: 1.211 ± 0.045
0.638TyrGln: 0.638 ± 0.029
1.345TyrArg: 1.345 ± 0.04
2.696TyrSer: 2.696 ± 0.061
2.068TyrThr: 2.068 ± 0.055
2.356TyrVal: 2.356 ± 0.051
0.285TyrTrp: 0.285 ± 0.018
1.715TyrTyr: 1.715 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2714 proteins (856979 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski