Bosea caraganae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5639 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370L739|A0A370L739_9BRAD Uncharacterized protein OS=Bosea caraganae OX=2763117 GN=DWE98_11885 PE=4 SV=1
MM1 pKa = 7.22TGAAGGAGGGEE12 pKa = 4.16NASGSAGGSGSGTAGGIGGPGGGSVGGTGGNGGGGATGGAGTADD56 pKa = 3.48SGSNGGLGGGGGGGAALVLNGSSLVVTTSGGVTGGIGGAGGGANSSFSGNLGNGGGGGAGIASASTGLQVSNSGTITGGAGGDD139 pKa = 3.2AGFLGFLGSGATNGVGGAGGTGVSLSGASASFTNTGAVRR178 pKa = 11.84GGGGGLLNTLNSFTGSGAGGDD199 pKa = 3.69GGAGLVISGAGGTVSNSGTIGGGTGGYY226 pKa = 10.45DD227 pKa = 3.12IDD229 pKa = 4.07WNVSQGGASGAGVILGGSGASLATGTVLTNNAGATIVGANRR270 pKa = 11.84AVMLAASGPDD280 pKa = 3.15NGGAGVSITGNYY292 pKa = 7.19NTINNAGTITGGRR305 pKa = 11.84AGNAGTAGVLVSGDD319 pKa = 3.37NNLVVSSGTITGGGAGGTIAPSVQFDD345 pKa = 3.42GHH347 pKa = 6.45NNTLEE352 pKa = 3.88LRR354 pKa = 11.84AGYY357 pKa = 10.65ALGGGAKK364 pKa = 10.36VNGTGGTLVLGGTANAAFDD383 pKa = 3.97LARR386 pKa = 11.84IGTSATSFNGFSAFNKK402 pKa = 9.39TGGSTWTLNGIYY414 pKa = 9.88TDD416 pKa = 3.51TSNWNVQQGTLAMASLAEE434 pKa = 4.25TTGTVSVASGAALSVLALSRR454 pKa = 11.84VHH456 pKa = 6.08GQVTIANGGTLVTTTTTSAAALDD479 pKa = 4.17GLTLSPNSILEE490 pKa = 4.21MNVTRR495 pKa = 11.84AGNTTGLLTLGSGGLVLDD513 pKa = 3.96GTLNITDD520 pKa = 4.16SGNALAAGTYY530 pKa = 10.41RR531 pKa = 11.84LFNVIGTLTNNTMVIGPAPMQYY553 pKa = 10.32LYY555 pKa = 10.98SIDD558 pKa = 3.3TSNPVFITLLVASNTSYY575 pKa = 11.09WNGAQATPNGTVNGGSGTWDD595 pKa = 2.94AAATNWTNATGTTSKK610 pKa = 10.86AWGGYY615 pKa = 6.98EE616 pKa = 4.69AVFQGTPGTVTVDD629 pKa = 3.11NGGVSAYY636 pKa = 8.02LTHH639 pKa = 6.77FLVDD643 pKa = 4.7GYY645 pKa = 8.73TLSGGPLTLASLSAAKK661 pKa = 10.02PIVEE665 pKa = 4.37VATGATATIQSVIAGTAGLDD685 pKa = 3.29KK686 pKa = 10.8TGAGTLTLTGANTYY700 pKa = 8.95TGNTSITAGTLQIAGSGSILGDD722 pKa = 3.39VANSGTLSFNPGSDD736 pKa = 2.63RR737 pKa = 11.84AFAGNISGTGSLTKK751 pKa = 10.65LGGSTLTLSIANTYY765 pKa = 10.77AGGTTVSAGVLNVRR779 pKa = 11.84NDD781 pKa = 3.31SALGTGAVSVASGAALEE798 pKa = 4.24VQGYY802 pKa = 10.12ISLPNALTLSGTGVANGGALRR823 pKa = 11.84NVSGANGLDD832 pKa = 3.69GAITLAGDD840 pKa = 3.38TRR842 pKa = 11.84INNDD846 pKa = 3.51SFDD849 pKa = 3.99SDD851 pKa = 3.68LGLVGGISGSGHH863 pKa = 5.01TLVLGGSSEE872 pKa = 4.81YY873 pKa = 9.0ITSVTGAIATGLGGVTIDD891 pKa = 3.29VTGAAGRR898 pKa = 11.84VILAGANTYY907 pKa = 9.92TGVTTVNSGTLQLMYY922 pKa = 10.53GAAIADD928 pKa = 3.76TGAVVVNAPGSLQIYY943 pKa = 9.14NSEE946 pKa = 4.6TIGSLAGAGSVVLNSGSTLAVGGDD970 pKa = 3.79DD971 pKa = 4.21SSTSFSGVISQSSGVGSLTKK991 pKa = 10.2IGTGSFTLSGTNTYY1005 pKa = 9.58TGATQVDD1012 pKa = 4.1GGEE1015 pKa = 4.03LRR1017 pKa = 11.84VNGSVQGTVGVASGATLGGSGAIAGAVTVASGGTLSAGSSPGTLTVGSLEE1067 pKa = 4.44LNSGANTVFEE1077 pKa = 4.88LNSPGVVGGGTNDD1090 pKa = 3.86HH1091 pKa = 6.95IIVNGSGAAGNLVLGGTLTANVASAGYY1118 pKa = 9.9YY1119 pKa = 10.26RR1120 pKa = 11.84LFDD1123 pKa = 3.6VTGGGAISGSFDD1135 pKa = 3.4TLALTAPSVAGATGTVYY1152 pKa = 10.58NAPSGVPTQVNLAVLGTSQAMQFWDD1177 pKa = 4.65GSDD1180 pKa = 3.2QLGNGTVDD1188 pKa = 3.6GGAGTWNAGNANWTGAPGQADD1209 pKa = 4.89FNAPWSDD1216 pKa = 3.41SVGVFF1221 pKa = 3.78

Molecular weight: 113.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370L5N6|A0A370L5N6_9BRAD L-glyceraldehyde 3-phosphate reductase OS=Bosea caraganae OX=2763117 GN=DWE98_12800 PE=4 SV=1
MM1 pKa = 7.88LGQTKK6 pKa = 9.07EE7 pKa = 3.54WWLEE11 pKa = 3.71PSIPPIWFRR20 pKa = 11.84FIMRR24 pKa = 11.84QFALAALLAVCCPAFSFANVPLEE47 pKa = 3.91QVSFQPQVRR56 pKa = 11.84GPGCLKK62 pKa = 10.45PEE64 pKa = 3.99AVAMIRR70 pKa = 11.84EE71 pKa = 4.76LVAKK75 pKa = 10.02IGPIQITSTCGGRR88 pKa = 11.84HH89 pKa = 5.23ARR91 pKa = 11.84RR92 pKa = 11.84SQHH95 pKa = 5.2YY96 pKa = 9.6SGNAVDD102 pKa = 4.9FRR104 pKa = 11.84PMATSARR111 pKa = 11.84KK112 pKa = 9.11AVAVAKK118 pKa = 10.35ALEE121 pKa = 4.35STGGVGSYY129 pKa = 10.65PNGVVHH135 pKa = 6.75VDD137 pKa = 2.98VGDD140 pKa = 4.53RR141 pKa = 11.84EE142 pKa = 4.38ISWFGHH148 pKa = 4.14KK149 pKa = 9.93RR150 pKa = 11.84ARR152 pKa = 11.84TRR154 pKa = 11.84LAYY157 pKa = 10.05AGRR160 pKa = 11.84SRR162 pKa = 11.84RR163 pKa = 3.65

Molecular weight: 17.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5639

0

5639

1791978

28

2763

317.8

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.26 ± 0.055

0.76 ± 0.01

5.332 ± 0.026

5.516 ± 0.033

3.703 ± 0.022

8.929 ± 0.054

1.875 ± 0.017

5.202 ± 0.023

3.236 ± 0.029

10.522 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.016

2.446 ± 0.021

5.336 ± 0.025

3.088 ± 0.018

7.071 ± 0.039

5.419 ± 0.026

5.201 ± 0.032

7.374 ± 0.027

1.291 ± 0.012

2.111 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski