Streptococcus satellite phage Javan482

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZP45|A0A4D5ZP45_9VIRU ArpU family encoded transcriptional regulator OS=Streptococcus satellite phage Javan482 OX=2558722 GN=JavanS482_0020 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.6APKK9 pKa = 10.6QFMFTYY15 pKa = 9.75QCKK18 pKa = 10.39DD19 pKa = 2.96YY20 pKa = 9.71DD21 pKa = 4.03TARR24 pKa = 11.84VTSTAILGYY33 pKa = 8.7ITGTYY38 pKa = 7.79EE39 pKa = 3.86QNLAEE44 pKa = 4.27ATLNGDD50 pKa = 3.15GDD52 pKa = 4.45LEE54 pKa = 4.24VTYY57 pKa = 10.96FEE59 pKa = 5.45DD60 pKa = 4.58KK61 pKa = 10.97SINFNLKK68 pKa = 10.17RR69 pKa = 11.84ICDD72 pKa = 3.8SFKK75 pKa = 10.88DD76 pKa = 3.81YY77 pKa = 11.31CNQPEE82 pKa = 4.1DD83 pKa = 3.82MEE85 pKa = 4.59GEE87 pKa = 4.27KK88 pKa = 10.75

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPG5|A0A4D5ZPG5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan482 OX=2558722 GN=JavanS482_0011 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.07IIEE5 pKa = 4.24YY6 pKa = 9.85KK7 pKa = 10.29KK8 pKa = 9.96KK9 pKa = 10.52NGTIVYY15 pKa = 9.09RR16 pKa = 11.84ANIYY20 pKa = 10.63LGIDD24 pKa = 3.12QVTGKK29 pKa = 10.02RR30 pKa = 11.84VKK32 pKa = 9.93TSITGRR38 pKa = 11.84TQTEE42 pKa = 4.35VKK44 pKa = 10.36QKK46 pKa = 10.9AKK48 pKa = 10.01HH49 pKa = 5.24AQFNFLSNGSTIKK62 pKa = 10.43RR63 pKa = 11.84KK64 pKa = 9.88VVIKK68 pKa = 8.87TFKK71 pKa = 10.26EE72 pKa = 4.46LSHH75 pKa = 6.68LWLEE79 pKa = 4.6TYY81 pKa = 10.71KK82 pKa = 10.43LTVKK86 pKa = 10.0PQTYY90 pKa = 9.55DD91 pKa = 3.09ATVTRR96 pKa = 11.84LNRR99 pKa = 11.84HH100 pKa = 5.38IMPTLGNMKK109 pKa = 9.51VDD111 pKa = 4.87KK112 pKa = 9.89ITASDD117 pKa = 3.12IQMLINRR124 pKa = 11.84LSKK127 pKa = 11.11YY128 pKa = 8.49YY129 pKa = 11.04VNYY132 pKa = 8.12TAVRR136 pKa = 11.84SVIRR140 pKa = 11.84KK141 pKa = 8.39VLQQGVLLGLIDD153 pKa = 4.2YY154 pKa = 10.54NPARR158 pKa = 11.84DD159 pKa = 3.45IILPRR164 pKa = 11.84KK165 pKa = 7.4QQNAKK170 pKa = 10.26KK171 pKa = 9.18KK172 pKa = 10.57VKK174 pKa = 10.54FIDD177 pKa = 4.16PSDD180 pKa = 3.48LKK182 pKa = 11.33SFLEE186 pKa = 4.19HH187 pKa = 7.06LEE189 pKa = 4.13SSQHH193 pKa = 5.59KK194 pKa = 10.1RR195 pKa = 11.84YY196 pKa = 10.19NLYY199 pKa = 10.34FDD201 pKa = 4.37AVLYY205 pKa = 10.4QLLLSTGLRR214 pKa = 11.84IGEE217 pKa = 4.13ACALEE222 pKa = 4.36WGDD225 pKa = 3.78IDD227 pKa = 5.14LKK229 pKa = 11.1KK230 pKa = 10.55GTITINKK237 pKa = 8.07TYY239 pKa = 10.96NKK241 pKa = 9.65NLKK244 pKa = 9.76FLSTAKK250 pKa = 8.49TQSGNRR256 pKa = 11.84VISIDD261 pKa = 3.32QKK263 pKa = 9.27TIRR266 pKa = 11.84SLKK269 pKa = 10.0LYY271 pKa = 8.45QVRR274 pKa = 11.84QRR276 pKa = 11.84QLFNEE281 pKa = 3.92VGARR285 pKa = 11.84VSEE288 pKa = 4.58VVFATPTRR296 pKa = 11.84KK297 pKa = 9.82YY298 pKa = 9.68FNASVRR304 pKa = 11.84QSALDD309 pKa = 3.79TRR311 pKa = 11.84CKK313 pKa = 9.91EE314 pKa = 3.83AGIEE318 pKa = 3.94RR319 pKa = 11.84FTFHH323 pKa = 7.65AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.51RR346 pKa = 11.84LGHH349 pKa = 6.41ANISMTLDD357 pKa = 3.43TYY359 pKa = 11.88GHH361 pKa = 6.98LSKK364 pKa = 11.01DD365 pKa = 3.47KK366 pKa = 10.63EE367 pKa = 4.32KK368 pKa = 11.01EE369 pKa = 3.74AVLYY373 pKa = 9.78YY374 pKa = 10.38EE375 pKa = 4.79KK376 pKa = 11.4AMNNLL381 pKa = 3.41

Molecular weight: 43.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3107

52

562

148.0

17.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.053 ± 0.453

0.515 ± 0.163

5.826 ± 0.461

8.722 ± 0.605

4.152 ± 0.313

4.635 ± 0.466

1.641 ± 0.21

7.242 ± 0.537

9.817 ± 0.564

9.527 ± 0.671

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.899 ± 0.129

5.536 ± 0.416

2.929 ± 0.582

4.731 ± 0.317

5.311 ± 0.437

5.246 ± 0.242

6.051 ± 0.443

4.957 ± 0.422

0.998 ± 0.186

5.214 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski