Mycobacterium phage Fulbright

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; unclassified Charlievirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EP72|A0A4Y6EP72_9CAUD HNH endonuclease OS=Mycobacterium phage Fulbright OX=2588511 GN=70 PE=4 SV=1
MM1 pKa = 7.47LPDD4 pKa = 4.77CALCGCPYY12 pKa = 10.63HH13 pKa = 7.24EE14 pKa = 4.9DD15 pKa = 3.27RR16 pKa = 11.84CRR18 pKa = 11.84TVVGAAEE25 pKa = 4.44YY26 pKa = 10.38IFGYY30 pKa = 10.85LPMFCDD36 pKa = 3.66CPGYY40 pKa = 10.42EE41 pKa = 4.28EE42 pKa = 5.72PEE44 pKa = 4.33DD45 pKa = 3.68ATT47 pKa = 4.17

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EVC2|A0A4Y6EVC2_9CAUD Uncharacterized protein OS=Mycobacterium phage Fulbright OX=2588511 GN=62 PE=4 SV=1
MM1 pKa = 6.95MAAATLTEE9 pKa = 4.06DD10 pKa = 4.44QRR12 pKa = 11.84WLLYY16 pKa = 9.56VAARR20 pKa = 11.84HH21 pKa = 5.28VMPIALLDD29 pKa = 3.78PDD31 pKa = 3.78YY32 pKa = 11.27GIRR35 pKa = 11.84GLKK38 pKa = 9.9ASHH41 pKa = 6.58GSPRR45 pKa = 11.84TFRR48 pKa = 11.84RR49 pKa = 11.84IFGRR53 pKa = 11.84CC54 pKa = 2.98

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13672

47

1046

195.3

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.178 ± 0.704

1.178 ± 0.155

6.736 ± 0.229

5.705 ± 0.333

2.809 ± 0.193

8.74 ± 0.449

2.092 ± 0.183

4.506 ± 0.171

2.911 ± 0.213

7.68 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.147

3.065 ± 0.239

5.749 ± 0.272

3.825 ± 0.215

7.022 ± 0.43

5.237 ± 0.187

6.949 ± 0.297

7.3 ± 0.254

1.865 ± 0.135

2.377 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski