Spironucleus salmonicida
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8098 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V6LS53|V6LS53_9EUKA 4Fe-4S ferredoxin iron-sulfur binding domain protein OS=Spironucleus salmonicida OX=348837 GN=SS50377_12391 PE=4 SV=1
MM1 pKa = 7.32 SAWDD5 pKa = 4.93 LITTHH10 pKa = 5.95 SQQNLDD16 pKa = 3.53 QMCFQPSTDD25 pKa = 3.35 VYY27 pKa = 11.1 RR28 pKa = 11.84 EE29 pKa = 4.06 DD30 pKa = 3.34 QLIPMDD36 pKa = 5.3 CSDD39 pKa = 3.63 QQASDD44 pKa = 3.62 GDD46 pKa = 3.41 IDD48 pKa = 3.66 QATRR52 pKa = 11.84 QTSYY56 pKa = 11.42 DD57 pKa = 3.73 GVSLAQQLKK66 pKa = 8.22 QQ67 pKa = 3.26
Molecular weight: 7.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.465
ProMoST 3.923
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.745
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.872
DTASelect 4.164
Thurlkill 3.567
EMBOSS 3.745
Sillero 3.821
Patrickios 1.914
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|V6LUI8|V6LUI8_9EUKA Uncharacterized protein OS=Spironucleus salmonicida OX=348837 GN=SS50377_11555 PE=4 SV=1
RR1 pKa = 7.92 QPVPPRR7 pKa = 11.84 PRR9 pKa = 11.84 PAGVPGRR16 pKa = 11.84 RR17 pKa = 11.84 GPGHH21 pKa = 6.95 AVRR24 pKa = 11.84 AVRR27 pKa = 11.84 APGARR32 pKa = 11.84 EE33 pKa = 3.84 RR34 pKa = 11.84 PRR36 pKa = 11.84 GALPGLPAAPPSAPRR51 pKa = 11.84 GPARR55 pKa = 11.84 HH56 pKa = 5.81 PQGVPGPCRR65 pKa = 11.84 GCTRR69 pKa = 11.84 LHH71 pKa = 4.98 GHH73 pKa = 6.37 ARR75 pKa = 11.84 AARR78 pKa = 11.84 ALPACRR84 pKa = 11.84 PRR86 pKa = 11.84 PSAPPAGVPGVRR98 pKa = 11.84 ARR100 pKa = 11.84 RR101 pKa = 11.84 VPGASAPPARR111 pKa = 11.84 PVRR114 pKa = 11.84 RR115 pKa = 11.84 SPRR118 pKa = 11.84 RR119 pKa = 11.84 HH120 pKa = 5.43 RR121 pKa = 11.84 RR122 pKa = 11.84 AGACRR127 pKa = 11.84 LRR129 pKa = 11.84 PAGAGPRR136 pKa = 11.84 EE137 pKa = 4.13 RR138 pKa = 11.84 GRR140 pKa = 11.84 RR141 pKa = 11.84 VPGALLLRR149 pKa = 11.84 LQPPPVRR156 pKa = 11.84 RR157 pKa = 11.84 GPTRR161 pKa = 11.84 HH162 pKa = 6.84 PLRR165 pKa = 11.84 LPRR168 pKa = 11.84 RR169 pKa = 11.84 ADD171 pKa = 3.09 GRR173 pKa = 11.84 PGHH176 pKa = 6.42 GRR178 pKa = 11.84 VQGPPPPGTPWRR190 pKa = 11.84 ALRR193 pKa = 11.84 VGAGGKK199 pKa = 9.56 RR200 pKa = 11.84 CWAPGRR206 pKa = 11.84 CPRR209 pKa = 11.84 WGG211 pKa = 3.21
Molecular weight: 22.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.371
IPC2_protein 10.804
IPC_protein 12.413
Toseland 12.574
ProMoST 13.086
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.091
Grimsley 12.618
Solomon 13.086
Lehninger 12.983
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.086
Sillero 12.574
Patrickios 11.798
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.187
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8098
0
8098
3053270
37
6144
377.0
43.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.273 ± 0.035
2.401 ± 0.039
4.998 ± 0.018
5.784 ± 0.026
5.23 ± 0.026
4.164 ± 0.031
1.69 ± 0.01
8.2 ± 0.033
6.882 ± 0.028
9.87 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.967 ± 0.011
6.01 ± 0.027
3.343 ± 0.022
8.29 ± 0.049
3.613 ± 0.023
7.478 ± 0.021
5.125 ± 0.019
5.5 ± 0.021
0.6 ± 0.008
3.581 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here