Bacillus phage Pascal

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Pagevirus; Bacillus virus Pascal

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RNJ3|A0A0A0RNJ3_9CAUD RNA polymerase sigma factor OS=Bacillus phage Pascal OX=1540092 GN=CPT_Pascal45 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 7.9PTNFICSPGIWSFGNYY18 pKa = 6.69STEE21 pKa = 4.46DD22 pKa = 3.54FTGMTPEE29 pKa = 4.64CEE31 pKa = 4.18ATLSALDD38 pKa = 3.74ARR40 pKa = 11.84IQAFEE45 pKa = 4.37EE46 pKa = 3.91EE47 pKa = 4.44LAAGKK52 pKa = 10.33AGG54 pKa = 3.34

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RSZ7|A0A0A0RSZ7_9CAUD Uncharacterized protein OS=Bacillus phage Pascal OX=1540092 GN=CPT_Pascal41 PE=4 SV=1
MM1 pKa = 7.46SFFTAEE7 pKa = 3.88QKK9 pKa = 10.95EE10 pKa = 4.13IMKK13 pKa = 10.4AKK15 pKa = 10.01EE16 pKa = 3.94ILPSTVMYY24 pKa = 10.7RR25 pKa = 11.84MKK27 pKa = 9.66TKK29 pKa = 10.83KK30 pKa = 7.96MTFEE34 pKa = 3.75AAIATKK40 pKa = 10.2EE41 pKa = 3.95PLSRR45 pKa = 11.84EE46 pKa = 3.98PRR48 pKa = 11.84LHH50 pKa = 6.38KK51 pKa = 10.67VLSAEE56 pKa = 4.5DD57 pKa = 3.16IKK59 pKa = 11.68VCVKK63 pKa = 10.59KK64 pKa = 10.85GIPRR68 pKa = 11.84KK69 pKa = 9.34ILSKK73 pKa = 10.06RR74 pKa = 11.84ACANWSRR81 pKa = 11.84EE82 pKa = 4.03KK83 pKa = 10.92IMNTPVKK90 pKa = 10.15KK91 pKa = 10.5KK92 pKa = 10.49SFFSKK97 pKa = 10.32RR98 pKa = 11.84QLANIKK104 pKa = 10.19RR105 pKa = 11.84LGIHH109 pKa = 5.59YY110 pKa = 7.44QTAVSRR116 pKa = 11.84VKK118 pKa = 9.58YY119 pKa = 8.24CHH121 pKa = 4.9WTKK124 pKa = 10.64EE125 pKa = 4.33RR126 pKa = 11.84ASSTPTGKK134 pKa = 10.3NGVAHH139 pKa = 7.02KK140 pKa = 10.65NN141 pKa = 3.36

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12687

37

1672

253.7

28.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.59 ± 0.348

0.788 ± 0.197

5.549 ± 0.211

6.952 ± 0.494

4.162 ± 0.208

6.928 ± 0.516

1.6 ± 0.173

5.801 ± 0.32

8.702 ± 0.438

7.37 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.198

6.645 ± 0.332

3.437 ± 0.416

5.084 ± 0.548

4.138 ± 0.211

5.683 ± 0.232

5.904 ± 0.432

5.659 ± 0.259

1.198 ± 0.139

4.036 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski