Proteiniborus sp. DW1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Proteiniborus; unclassified Proteiniborus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2967 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4M3V7|A0A1M4M3V7_9FIRM Isoleucine--tRNA ligase OS=Proteiniborus sp. DW1 OX=1889883 GN=ileS PE=3 SV=1
MM1 pKa = 7.4YY2 pKa = 10.33YY3 pKa = 10.72GLSDD7 pKa = 3.4HH8 pKa = 7.14QSFVDD13 pKa = 3.81NNICGVTIGQEE24 pKa = 4.01NLNDD28 pKa = 2.92IHH30 pKa = 6.36TSKK33 pKa = 10.11DD34 pKa = 3.25TNDD37 pKa = 3.96DD38 pKa = 3.12MRR40 pKa = 11.84SVYY43 pKa = 10.16KK44 pKa = 10.57YY45 pKa = 11.02SNTEE49 pKa = 3.21EE50 pKa = 5.32DD51 pKa = 3.75IINLIEE57 pKa = 4.21EE58 pKa = 4.77LDD60 pKa = 3.85LEE62 pKa = 4.25EE63 pKa = 6.04SIEE66 pKa = 5.04KK67 pKa = 10.12IINDD71 pKa = 4.03LGII74 pKa = 4.11

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4M6F5|A0A1M4M6F5_9FIRM Purine nucleoside phosphorylase OS=Proteiniborus sp. DW1 OX=1889883 GN=DW1_1652 PE=3 SV=1
MM1 pKa = 7.76KK2 pKa = 10.33GRR4 pKa = 11.84FVSGIVTGSLIGATAGMLAMTRR26 pKa = 11.84MSPRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.28MMRR37 pKa = 11.84VGKK40 pKa = 9.95RR41 pKa = 11.84IASTVVSNIGLFF53 pKa = 3.47

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2967

0

2967

895212

29

2054

301.7

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.817 ± 0.044

0.963 ± 0.017

5.571 ± 0.035

7.721 ± 0.048

4.199 ± 0.036

6.608 ± 0.04

1.469 ± 0.017

10.139 ± 0.051

8.227 ± 0.046

9.313 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.022

5.604 ± 0.035

3.025 ± 0.024

2.456 ± 0.023

3.9 ± 0.031

6.138 ± 0.036

4.939 ± 0.029

6.638 ± 0.04

0.665 ± 0.013

4.005 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski