Lily virus X
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4LD43|Q4LD43_LVX Movement protein TGBp3 OS=Lily virus X OX=12194 PE=3 SV=1
MM1 pKa = 6.63 TTFVPDD7 pKa = 3.43 AKK9 pKa = 9.8 TWADD13 pKa = 3.34 TAYY16 pKa = 8.26 TAQSEE21 pKa = 4.83 SVATAEE27 pKa = 4.23 EE28 pKa = 4.25 LQSIATLWEE37 pKa = 5.03 GIGIPAANFFDD48 pKa = 4.52 VAFQLAMRR56 pKa = 11.84 CSDD59 pKa = 3.12 GHH61 pKa = 6.22 ASSLTVLSGNCTVAPTVTLKK81 pKa = 10.95 AAAGLVKK88 pKa = 10.46 AVLPLRR94 pKa = 11.84 QFCRR98 pKa = 11.84 YY99 pKa = 7.73 YY100 pKa = 11.4 AKK102 pKa = 10.48 FVWNWRR108 pKa = 11.84 LSHH111 pKa = 7.1 DD112 pKa = 4.46 LPPANWADD120 pKa = 3.5 SQFPAEE126 pKa = 4.09 ARR128 pKa = 11.84 FAAFDD133 pKa = 3.83 FFDD136 pKa = 4.1 GVTNSAAPQPPDD148 pKa = 3.32 GLIRR152 pKa = 11.84 PPTEE156 pKa = 4.36 LEE158 pKa = 3.69 LSAAQTAKK166 pKa = 10.14 FAALARR172 pKa = 11.84 VRR174 pKa = 11.84 GSGFVTTAAEE184 pKa = 4.18 ITHH187 pKa = 5.79 GRR189 pKa = 11.84 AEE191 pKa = 4.27 VSRR194 pKa = 11.84 TMLLSPPP201 pKa = 3.88
Molecular weight: 21.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.417
IPC2_protein 5.474
IPC_protein 5.397
Toseland 5.55
ProMoST 5.639
Dawson 5.512
Bjellqvist 5.575
Wikipedia 5.448
Rodwell 5.461
Grimsley 5.575
Solomon 5.512
Lehninger 5.486
Nozaki 5.703
DTASelect 5.868
Thurlkill 5.703
EMBOSS 5.677
Sillero 5.779
Patrickios 4.24
IPC_peptide 5.525
IPC2_peptide 5.792
IPC2.peptide.svr19 5.806
Protein with the highest isoelectric point:
>tr|Q4LD45|Q4LD45_LVX Triple gene block protein 1 OS=Lily virus X OX=12194 PE=4 SV=1
MM1 pKa = 7.95 PLTPPPDD8 pKa = 3.42 YY9 pKa = 9.37 TKK11 pKa = 10.79 PFIAVVVGGTLAAFVLLLTRR31 pKa = 11.84 NTLPHH36 pKa = 6.49 TGDD39 pKa = 3.73 NLHH42 pKa = 6.53 SLPHH46 pKa = 6.52 GGTYY50 pKa = 10.36 CDD52 pKa = 3.16 GTKK55 pKa = 10.13 RR56 pKa = 11.84 IRR58 pKa = 11.84 YY59 pKa = 7.05 GGPHH63 pKa = 7.05 RR64 pKa = 11.84 SHH66 pKa = 8.1 VPEE69 pKa = 5.06 LPAKK73 pKa = 8.75 SWALITVVAILIALHH88 pKa = 6.21 FSCLRR93 pKa = 11.84 THH95 pKa = 6.89 RR96 pKa = 11.84 VHH98 pKa = 7.47 RR99 pKa = 11.84 CVLCHH104 pKa = 5.44 TTSGG108 pKa = 3.54
Molecular weight: 11.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.975
IPC2_protein 8.931
IPC_protein 9.092
Toseland 9.311
ProMoST 9.297
Dawson 9.663
Bjellqvist 9.619
Wikipedia 9.853
Rodwell 9.765
Grimsley 9.692
Solomon 9.794
Lehninger 9.765
Nozaki 9.765
DTASelect 9.487
Thurlkill 9.545
EMBOSS 9.794
Sillero 9.736
Patrickios 4.8
IPC_peptide 9.78
IPC2_peptide 9.048
IPC2.peptide.svr19 7.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1901
78
1298
380.2
42.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.469 ± 1.65
2.052 ± 0.641
5.313 ± 0.654
4.892 ± 0.841
4.208 ± 0.457
5.576 ± 0.558
4.261 ± 0.782
5.26 ± 0.804
4.366 ± 1.266
9.732 ± 0.557
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.946 ± 0.507
3.051 ± 0.324
6.733 ± 0.579
4.05 ± 1.036
4.997 ± 0.323
5.26 ± 0.52
7.996 ± 0.784
6.365 ± 0.928
1.105 ± 0.262
3.367 ± 0.449
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here