Enterococcus phage Nonaheksakonda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9U8A6|A0A3S9U8A6_9CAUD Uncharacterized protein OS=Enterococcus phage Nonaheksakonda OX=2488858 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.46KK3 pKa = 9.16EE4 pKa = 4.03AKK6 pKa = 9.44FSKK9 pKa = 10.49EE10 pKa = 3.48VVEE13 pKa = 4.04YY14 pKa = 10.22LKK16 pKa = 11.29SKK18 pKa = 9.99GAVVNVNTATIYY30 pKa = 10.95DD31 pKa = 3.67RR32 pKa = 11.84VGRR35 pKa = 11.84ADD37 pKa = 4.15IEE39 pKa = 4.33ACYY42 pKa = 10.25SGYY45 pKa = 10.6FIALEE50 pKa = 4.3LKK52 pKa = 9.02TGNYY56 pKa = 9.45QPDD59 pKa = 3.98PLQIRR64 pKa = 11.84YY65 pKa = 8.61LQGVRR70 pKa = 11.84CAGGYY75 pKa = 10.44GLLLRR80 pKa = 11.84DD81 pKa = 4.03TLDD84 pKa = 3.91DD85 pKa = 5.44LEE87 pKa = 6.28DD88 pKa = 3.87LLLYY92 pKa = 9.93LDD94 pKa = 4.06NMEE97 pKa = 4.21SAVYY101 pKa = 10.28YY102 pKa = 10.56GADD105 pKa = 3.42DD106 pKa = 3.74IYY108 pKa = 11.32FNYY111 pKa = 8.83EE112 pKa = 3.88QPDD115 pKa = 3.94LPDD118 pKa = 3.59IADD121 pKa = 4.24DD122 pKa = 4.8KK123 pKa = 11.82LEE125 pKa = 3.88VWYY128 pKa = 9.78DD129 pKa = 3.27

Molecular weight: 14.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9U801|A0A3S9U801_9CAUD Uncharacterized protein OS=Enterococcus phage Nonaheksakonda OX=2488858 PE=4 SV=1
MM1 pKa = 7.84PAPKK5 pKa = 9.78PIMVKK10 pKa = 10.03DD11 pKa = 3.87LRR13 pKa = 11.84TQQTRR18 pKa = 11.84VFKK21 pKa = 10.69SQSEE25 pKa = 3.86ADD27 pKa = 3.15RR28 pKa = 11.84FYY30 pKa = 11.36NKK32 pKa = 9.97KK33 pKa = 9.9SGYY36 pKa = 10.0FKK38 pKa = 10.66DD39 pKa = 3.7VKK41 pKa = 10.21TKK43 pKa = 10.55LGGKK47 pKa = 8.0NRR49 pKa = 11.84HH50 pKa = 5.09YY51 pKa = 10.77EE52 pKa = 4.06IIQVVV57 pKa = 3.15

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

12852

44

1295

173.7

19.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.618 ± 0.436

0.778 ± 0.119

6.38 ± 0.228

8.302 ± 0.405

3.89 ± 0.228

6.155 ± 0.504

1.533 ± 0.18

6.855 ± 0.244

8.785 ± 0.335

8.621 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.058 ± 0.215

6.287 ± 0.255

2.817 ± 0.236

3.711 ± 0.218

3.68 ± 0.155

5.174 ± 0.209

5.945 ± 0.33

6.676 ± 0.227

1.346 ± 0.132

4.388 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski