Streptococcus satellite phage Javan357
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZL93|A0A4D5ZL93_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan357 OX=2558657 GN=JavanS357_0008 PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.96 KK3 pKa = 10.91 NMTLDD8 pKa = 4.41 LDD10 pKa = 4.09 NMTQSEE16 pKa = 4.5 FDD18 pKa = 3.49 NRR20 pKa = 11.84 ITEE23 pKa = 4.12 IKK25 pKa = 10.3 DD26 pKa = 3.2 RR27 pKa = 11.84 NPNLFQFIIDD37 pKa = 3.88 FLDD40 pKa = 4.06 DD41 pKa = 3.54 KK42 pKa = 10.4 VTPEE46 pKa = 3.89 EE47 pKa = 4.34 VYY49 pKa = 11.05 DD50 pKa = 3.84 FLKK53 pKa = 10.18 MEE55 pKa = 4.85 RR56 pKa = 11.84 SYY58 pKa = 11.18 QVNYY62 pKa = 9.19 IKK64 pKa = 10.55 NYY66 pKa = 8.5 KK67 pKa = 9.6 ARR69 pKa = 11.84 AA70 pKa = 3.49
Molecular weight: 8.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.718
IPC2_protein 4.711
IPC_protein 4.546
Toseland 4.355
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.393
Rodwell 4.368
Grimsley 4.279
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.8
Thurlkill 4.38
EMBOSS 4.406
Sillero 4.647
Patrickios 4.139
IPC_peptide 4.495
IPC2_peptide 4.635
IPC2.peptide.svr19 4.634
Protein with the highest isoelectric point:
>tr|A0A4D5ZKH4|A0A4D5ZKH4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan357 OX=2558657 GN=JavanS357_0011 PE=4 SV=1
MM1 pKa = 7.65 EE2 pKa = 5.23 NLIEE6 pKa = 4.51 SDD8 pKa = 3.63 LVIFKK13 pKa = 9.43 MRR15 pKa = 11.84 YY16 pKa = 9.19 LEE18 pKa = 5.9 AGATWEE24 pKa = 4.69 DD25 pKa = 3.61 VAEE28 pKa = 4.52 KK29 pKa = 10.76 LNKK32 pKa = 5.44 TTRR35 pKa = 11.84 YY36 pKa = 8.12 INSRR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 9.58 VIAKK46 pKa = 9.92 RR47 pKa = 11.84 FVEE50 pKa = 4.28 LKK52 pKa = 10.67 GYY54 pKa = 10.26
Molecular weight: 6.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.023
IPC2_protein 8.873
IPC_protein 8.916
Toseland 9.56
ProMoST 9.385
Dawson 9.794
Bjellqvist 9.458
Wikipedia 9.97
Rodwell 10.116
Grimsley 9.882
Solomon 9.897
Lehninger 9.867
Nozaki 9.502
DTASelect 9.458
Thurlkill 9.633
EMBOSS 9.97
Sillero 9.706
Patrickios 7.644
IPC_peptide 9.882
IPC2_peptide 7.966
IPC2.peptide.svr19 7.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2490
53
388
166.0
19.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.096 ± 0.385
0.602 ± 0.099
6.506 ± 0.553
8.193 ± 0.773
4.739 ± 0.42
4.699 ± 0.657
1.727 ± 0.38
6.426 ± 0.27
11.325 ± 0.634
10.0 ± 0.537
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.129 ± 0.309
6.386 ± 0.633
1.968 ± 0.269
3.695 ± 0.376
4.578 ± 0.258
5.703 ± 0.466
7.068 ± 0.677
5.382 ± 0.466
0.803 ± 0.116
3.976 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here