Winogradskyella endarachnes

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L6U7W8|A0A6L6U7W8_9FLAO Thioredoxin domain-containing protein OS=Winogradskyella endarachnes OX=2681965 GN=GN138_00860 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.23YY4 pKa = 10.58FKK6 pKa = 10.94LFLFLFVVTITSVTVVSCAEE26 pKa = 4.27DD27 pKa = 3.73GVNGLDD33 pKa = 3.93GVDD36 pKa = 4.83GIDD39 pKa = 4.79GEE41 pKa = 4.56DD42 pKa = 4.08GEE44 pKa = 5.47DD45 pKa = 4.65GEE47 pKa = 5.57DD48 pKa = 3.45LTIEE52 pKa = 3.87PTIFEE57 pKa = 4.79NKK59 pKa = 9.84SSLQPLVSIQPQFNYY74 pKa = 10.16VEE76 pKa = 4.58AYY78 pKa = 10.5SLISSTDD85 pKa = 3.17EE86 pKa = 3.9LADD89 pKa = 3.74GFKK92 pKa = 11.08LLGAQDD98 pKa = 3.85GAGFLQDD105 pKa = 3.26GDD107 pKa = 3.94GYY109 pKa = 10.35IYY111 pKa = 10.84VVNSEE116 pKa = 4.47DD117 pKa = 3.3DD118 pKa = 3.64HH119 pKa = 7.61AVSRR123 pKa = 11.84IHH125 pKa = 6.85FDD127 pKa = 2.85EE128 pKa = 4.27YY129 pKa = 10.9MNPTGGEE136 pKa = 3.83WLLTDD141 pKa = 4.89DD142 pKa = 4.41VADD145 pKa = 4.15EE146 pKa = 4.55ARR148 pKa = 11.84QCSATMWEE156 pKa = 4.18AAIHH160 pKa = 6.3GGSQDD165 pKa = 3.05IFLSASEE172 pKa = 4.46SINYY176 pKa = 8.62DD177 pKa = 3.25VKK179 pKa = 11.41GIDD182 pKa = 3.62PWVEE186 pKa = 4.2TPTPTADD193 pKa = 3.7FGLDD197 pKa = 3.22ALGEE201 pKa = 4.24FSWEE205 pKa = 4.04NAVPLPKK212 pKa = 9.94DD213 pKa = 3.3AYY215 pKa = 10.24SGKK218 pKa = 8.28TVIIGGDD225 pKa = 3.61DD226 pKa = 3.63DD227 pKa = 5.48SSGSEE232 pKa = 4.05GQVILYY238 pKa = 10.09YY239 pKa = 10.42SEE241 pKa = 5.72NGDD244 pKa = 3.78ADD246 pKa = 3.86LTGGKK251 pKa = 9.38IYY253 pKa = 10.49VLKK256 pKa = 10.69LKK258 pKa = 10.13EE259 pKa = 4.07VSNSAGGVQDD269 pKa = 3.74VVAGTIYY276 pKa = 10.94NEE278 pKa = 4.36SNLDD282 pKa = 3.53LQVSYY287 pKa = 10.56EE288 pKa = 4.08VEE290 pKa = 3.86FVEE293 pKa = 4.79IVNGAAMTKK302 pKa = 10.59NEE304 pKa = 4.25MEE306 pKa = 4.49DD307 pKa = 3.12ACVAVQASAFMRR319 pKa = 11.84VEE321 pKa = 3.89DD322 pKa = 3.72VDD324 pKa = 3.94YY325 pKa = 11.6QKK327 pKa = 11.71GDD329 pKa = 3.53DD330 pKa = 3.62ANARR334 pKa = 11.84NVFFAVTGRR343 pKa = 11.84GPNANTYY350 pKa = 9.97NDD352 pKa = 2.99WGTIYY357 pKa = 10.93KK358 pKa = 10.5LEE360 pKa = 5.23LDD362 pKa = 4.69DD363 pKa = 6.63DD364 pKa = 4.57SPLTGSLTQIVSGNTEE380 pKa = 3.92TNNADD385 pKa = 3.64GNNSLLQSPDD395 pKa = 3.75NICVTEE401 pKa = 4.0NFVYY405 pKa = 10.65YY406 pKa = 10.52QEE408 pKa = 5.3DD409 pKa = 3.86PNSFDD414 pKa = 4.18RR415 pKa = 11.84NHH417 pKa = 6.81AAYY420 pKa = 9.4IYY422 pKa = 7.53QTNLNGDD429 pKa = 3.55NSHH432 pKa = 6.4VVLEE436 pKa = 4.1LLVRR440 pKa = 11.84QDD442 pKa = 4.28LDD444 pKa = 3.73PTGSTGYY451 pKa = 10.06SGEE454 pKa = 4.08FGALIDD460 pKa = 4.18VSDD463 pKa = 4.97KK464 pKa = 11.15LGQPDD469 pKa = 4.11TFMLALQPHH478 pKa = 5.67YY479 pKa = 10.04WEE481 pKa = 5.1SDD483 pKa = 3.41DD484 pKa = 4.96FVVDD488 pKa = 5.05GLPDD492 pKa = 3.77DD493 pKa = 3.73QGGQIILLKK502 pKa = 10.5GLPRR506 pKa = 5.12

Molecular weight: 54.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L6UBG5|A0A6L6UBG5_9FLAO Uncharacterized protein OS=Winogradskyella endarachnes OX=2681965 GN=GN138_12760 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.65LSVSTEE48 pKa = 3.95SRR50 pKa = 11.84HH51 pKa = 6.01KK52 pKa = 10.6KK53 pKa = 9.6

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3122

0

3122

1080222

38

2737

346.0

39.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.04

0.753 ± 0.014

5.833 ± 0.035

6.595 ± 0.045

5.196 ± 0.036

6.185 ± 0.049

1.694 ± 0.021

8.222 ± 0.04

7.74 ± 0.067

9.243 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.021

6.585 ± 0.048

3.171 ± 0.022

3.231 ± 0.019

3.105 ± 0.035

6.687 ± 0.042

6.156 ± 0.059

6.183 ± 0.033

0.963 ± 0.015

4.156 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski