Amino acid dipepetide frequency for Mycobacterium phage DD5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.181AlaAla: 12.181 ± 1.382
0.687AlaCys: 0.687 ± 0.209
6.559AlaAsp: 6.559 ± 0.571
6.122AlaGlu: 6.122 ± 0.731
3.123AlaPhe: 3.123 ± 0.511
7.746AlaGly: 7.746 ± 0.975
1.811AlaHis: 1.811 ± 0.343
3.998AlaIle: 3.998 ± 0.489
4.373AlaLys: 4.373 ± 0.62
8.683AlaLeu: 8.683 ± 0.916
2.748AlaMet: 2.748 ± 0.413
2.374AlaAsn: 2.374 ± 0.429
4.747AlaPro: 4.747 ± 0.754
2.686AlaGln: 2.686 ± 0.491
6.122AlaArg: 6.122 ± 0.572
5.06AlaSer: 5.06 ± 0.612
5.247AlaThr: 5.247 ± 0.723
8.495AlaVal: 8.495 ± 0.797
1.624AlaTrp: 1.624 ± 0.309
2.873AlaTyr: 2.873 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.216
0.0CysCys: 0.0 ± 0.0
0.562CysAsp: 0.562 ± 0.19
0.625CysGlu: 0.625 ± 0.194
0.125CysPhe: 0.125 ± 0.087
0.75CysGly: 0.75 ± 0.248
0.125CysHis: 0.125 ± 0.088
0.187CysIle: 0.187 ± 0.12
0.375CysLys: 0.375 ± 0.201
0.5CysLeu: 0.5 ± 0.202
0.062CysMet: 0.062 ± 0.063
0.187CysAsn: 0.187 ± 0.112
0.312CysPro: 0.312 ± 0.122
0.312CysGln: 0.312 ± 0.146
0.625CysArg: 0.625 ± 0.23
0.5CysSer: 0.5 ± 0.178
0.375CysThr: 0.375 ± 0.158
0.375CysVal: 0.375 ± 0.142
0.25CysTrp: 0.25 ± 0.125
0.062CysTyr: 0.062 ± 0.065
0.0CysXaa: 0.0 ± 0.0
Asp
6.246AspAla: 6.246 ± 0.447
0.5AspCys: 0.5 ± 0.185
4.81AspAsp: 4.81 ± 0.574
3.685AspGlu: 3.685 ± 0.573
2.624AspPhe: 2.624 ± 0.371
6.246AspGly: 6.246 ± 0.624
1.187AspHis: 1.187 ± 0.308
2.936AspIle: 2.936 ± 0.445
2.811AspLys: 2.811 ± 0.491
6.934AspLeu: 6.934 ± 0.751
1.374AspMet: 1.374 ± 0.261
1.936AspAsn: 1.936 ± 0.418
4.81AspPro: 4.81 ± 0.574
1.624AspGln: 1.624 ± 0.371
3.248AspArg: 3.248 ± 0.43
2.998AspSer: 2.998 ± 0.52
3.623AspThr: 3.623 ± 0.373
4.56AspVal: 4.56 ± 0.572
2.186AspTrp: 2.186 ± 0.374
1.874AspTyr: 1.874 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
5.684GluAla: 5.684 ± 0.731
0.312GluCys: 0.312 ± 0.127
4.935GluAsp: 4.935 ± 0.515
4.81GluGlu: 4.81 ± 0.71
2.124GluPhe: 2.124 ± 0.374
4.373GluGly: 4.373 ± 0.614
1.624GluHis: 1.624 ± 0.331
3.436GluIle: 3.436 ± 0.482
2.374GluLys: 2.374 ± 0.424
6.309GluLeu: 6.309 ± 0.605
1.562GluMet: 1.562 ± 0.288
1.749GluAsn: 1.749 ± 0.386
2.748GluPro: 2.748 ± 0.396
2.748GluGln: 2.748 ± 0.516
4.248GluArg: 4.248 ± 0.625
3.248GluSer: 3.248 ± 0.423
3.81GluThr: 3.81 ± 0.535
5.372GluVal: 5.372 ± 0.746
1.437GluTrp: 1.437 ± 0.358
2.374GluTyr: 2.374 ± 0.487
0.0GluXaa: 0.0 ± 0.0
Phe
2.436PheAla: 2.436 ± 0.46
0.375PheCys: 0.375 ± 0.167
3.061PheAsp: 3.061 ± 0.368
2.311PheGlu: 2.311 ± 0.328
0.687PhePhe: 0.687 ± 0.194
3.748PheGly: 3.748 ± 0.502
0.687PheHis: 0.687 ± 0.275
1.374PheIle: 1.374 ± 0.279
1.437PheLys: 1.437 ± 0.318
2.311PheLeu: 2.311 ± 0.446
0.5PheMet: 0.5 ± 0.196
1.124PheAsn: 1.124 ± 0.326
1.624PhePro: 1.624 ± 0.324
0.75PheGln: 0.75 ± 0.191
1.874PheArg: 1.874 ± 0.381
1.874PheSer: 1.874 ± 0.301
2.061PheThr: 2.061 ± 0.333
2.124PheVal: 2.124 ± 0.415
0.5PheTrp: 0.5 ± 0.162
0.999PheTyr: 0.999 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
7.808GlyAla: 7.808 ± 1.158
0.625GlyCys: 0.625 ± 0.203
6.059GlyAsp: 6.059 ± 0.65
4.81GlyGlu: 4.81 ± 0.535
2.873GlyPhe: 2.873 ± 0.441
10.557GlyGly: 10.557 ± 2.741
1.936GlyHis: 1.936 ± 0.451
4.248GlyIle: 4.248 ± 0.704
3.998GlyLys: 3.998 ± 0.507
7.121GlyLeu: 7.121 ± 0.748
1.999GlyMet: 1.999 ± 0.398
3.373GlyAsn: 3.373 ± 0.422
3.685GlyPro: 3.685 ± 0.592
2.561GlyGln: 2.561 ± 0.383
4.872GlyArg: 4.872 ± 0.556
6.434GlySer: 6.434 ± 0.829
5.122GlyThr: 5.122 ± 0.673
5.31GlyVal: 5.31 ± 0.496
2.499GlyTrp: 2.499 ± 0.425
2.998GlyTyr: 2.998 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
1.999HisAla: 1.999 ± 0.454
0.187HisCys: 0.187 ± 0.148
1.374HisAsp: 1.374 ± 0.273
1.499HisGlu: 1.499 ± 0.298
0.625HisPhe: 0.625 ± 0.2
1.499HisGly: 1.499 ± 0.399
0.687HisHis: 0.687 ± 0.254
0.812HisIle: 0.812 ± 0.193
0.937HisLys: 0.937 ± 0.307
1.811HisLeu: 1.811 ± 0.377
0.187HisMet: 0.187 ± 0.105
0.312HisAsn: 0.312 ± 0.129
1.499HisPro: 1.499 ± 0.301
1.124HisGln: 1.124 ± 0.264
1.374HisArg: 1.374 ± 0.269
0.687HisSer: 0.687 ± 0.202
1.062HisThr: 1.062 ± 0.259
1.811HisVal: 1.811 ± 0.345
0.562HisTrp: 0.562 ± 0.179
0.562HisTyr: 0.562 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
5.559IleAla: 5.559 ± 0.623
0.25IleCys: 0.25 ± 0.113
3.248IleAsp: 3.248 ± 0.413
3.56IleGlu: 3.56 ± 0.443
1.124IlePhe: 1.124 ± 0.244
3.873IleGly: 3.873 ± 0.442
0.75IleHis: 0.75 ± 0.231
1.374IleIle: 1.374 ± 0.246
1.936IleLys: 1.936 ± 0.321
3.498IleLeu: 3.498 ± 0.419
0.687IleMet: 0.687 ± 0.199
1.936IleAsn: 1.936 ± 0.318
3.186IlePro: 3.186 ± 0.376
1.437IleGln: 1.437 ± 0.352
3.623IleArg: 3.623 ± 0.444
3.311IleSer: 3.311 ± 0.577
3.56IleThr: 3.56 ± 0.456
2.873IleVal: 2.873 ± 0.626
0.687IleTrp: 0.687 ± 0.176
1.499IleTyr: 1.499 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
4.123LysAla: 4.123 ± 0.553
0.437LysCys: 0.437 ± 0.147
2.499LysAsp: 2.499 ± 0.447
2.249LysGlu: 2.249 ± 0.376
1.312LysPhe: 1.312 ± 0.309
2.686LysGly: 2.686 ± 0.418
1.062LysHis: 1.062 ± 0.34
2.311LysIle: 2.311 ± 0.464
1.624LysLys: 1.624 ± 0.38
3.248LysLeu: 3.248 ± 0.483
1.062LysMet: 1.062 ± 0.234
1.374LysAsn: 1.374 ± 0.292
2.499LysPro: 2.499 ± 0.432
1.874LysGln: 1.874 ± 0.403
3.248LysArg: 3.248 ± 0.472
2.311LysSer: 2.311 ± 0.361
2.748LysThr: 2.748 ± 0.443
3.436LysVal: 3.436 ± 0.502
0.625LysTrp: 0.625 ± 0.213
1.187LysTyr: 1.187 ± 0.274
0.0LysXaa: 0.0 ± 0.0
Leu
8.87LeuAla: 8.87 ± 0.894
0.312LeuCys: 0.312 ± 0.129
6.059LeuAsp: 6.059 ± 0.579
5.434LeuGlu: 5.434 ± 0.612
2.249LeuPhe: 2.249 ± 0.398
6.559LeuGly: 6.559 ± 0.704
1.499LeuHis: 1.499 ± 0.374
4.81LeuIle: 4.81 ± 0.476
4.248LeuLys: 4.248 ± 0.498
5.872LeuLeu: 5.872 ± 0.606
1.624LeuMet: 1.624 ± 0.31
3.123LeuAsn: 3.123 ± 0.476
5.559LeuPro: 5.559 ± 0.524
2.873LeuGln: 2.873 ± 0.546
5.747LeuArg: 5.747 ± 0.542
5.434LeuSer: 5.434 ± 0.541
5.872LeuThr: 5.872 ± 0.449
4.747LeuVal: 4.747 ± 0.73
0.812LeuTrp: 0.812 ± 0.303
2.436LeuTyr: 2.436 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
2.499MetAla: 2.499 ± 0.407
0.0MetCys: 0.0 ± 0.0
0.937MetAsp: 0.937 ± 0.266
1.562MetGlu: 1.562 ± 0.324
0.687MetPhe: 0.687 ± 0.168
1.811MetGly: 1.811 ± 0.324
0.312MetHis: 0.312 ± 0.137
0.625MetIle: 0.625 ± 0.21
1.249MetLys: 1.249 ± 0.299
1.062MetLeu: 1.062 ± 0.283
0.187MetMet: 0.187 ± 0.11
1.062MetAsn: 1.062 ± 0.222
1.124MetPro: 1.124 ± 0.283
0.562MetGln: 0.562 ± 0.206
0.999MetArg: 0.999 ± 0.217
1.624MetSer: 1.624 ± 0.323
2.186MetThr: 2.186 ± 0.373
0.999MetVal: 0.999 ± 0.282
0.187MetTrp: 0.187 ± 0.115
0.5MetTyr: 0.5 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.186AsnAla: 3.186 ± 0.47
0.062AsnCys: 0.062 ± 0.067
2.061AsnAsp: 2.061 ± 0.358
1.811AsnGlu: 1.811 ± 0.386
0.999AsnPhe: 0.999 ± 0.272
3.436AsnGly: 3.436 ± 0.472
0.75AsnHis: 0.75 ± 0.186
1.749AsnIle: 1.749 ± 0.333
0.687AsnLys: 0.687 ± 0.239
2.686AsnLeu: 2.686 ± 0.384
0.5AsnMet: 0.5 ± 0.163
0.812AsnAsn: 0.812 ± 0.225
2.811AsnPro: 2.811 ± 0.369
1.062AsnGln: 1.062 ± 0.226
1.374AsnArg: 1.374 ± 0.357
1.811AsnSer: 1.811 ± 0.361
1.811AsnThr: 1.811 ± 0.35
2.499AsnVal: 2.499 ± 0.424
0.75AsnTrp: 0.75 ± 0.214
1.249AsnTyr: 1.249 ± 0.283
0.0AsnXaa: 0.0 ± 0.0
Pro
5.247ProAla: 5.247 ± 0.571
0.437ProCys: 0.437 ± 0.153
4.185ProAsp: 4.185 ± 0.57
4.248ProGlu: 4.248 ± 0.463
2.249ProPhe: 2.249 ± 0.425
5.06ProGly: 5.06 ± 0.583
1.249ProHis: 1.249 ± 0.331
2.561ProIle: 2.561 ± 0.41
1.936ProLys: 1.936 ± 0.315
4.497ProLeu: 4.497 ± 0.615
1.062ProMet: 1.062 ± 0.271
1.624ProAsn: 1.624 ± 0.348
3.248ProPro: 3.248 ± 0.557
1.687ProGln: 1.687 ± 0.409
2.873ProArg: 2.873 ± 0.565
4.06ProSer: 4.06 ± 0.579
3.436ProThr: 3.436 ± 0.527
3.748ProVal: 3.748 ± 0.461
0.812ProTrp: 0.812 ± 0.298
1.562ProTyr: 1.562 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
2.561GlnAla: 2.561 ± 0.461
0.25GlnCys: 0.25 ± 0.112
1.499GlnAsp: 1.499 ± 0.348
1.624GlnGlu: 1.624 ± 0.284
1.249GlnPhe: 1.249 ± 0.275
2.374GlnGly: 2.374 ± 0.298
0.5GlnHis: 0.5 ± 0.165
2.436GlnIle: 2.436 ± 0.484
1.312GlnLys: 1.312 ± 0.288
3.81GlnLeu: 3.81 ± 0.554
1.124GlnMet: 1.124 ± 0.278
0.5GlnAsn: 0.5 ± 0.148
1.999GlnPro: 1.999 ± 0.409
1.811GlnGln: 1.811 ± 0.399
1.811GlnArg: 1.811 ± 0.358
1.936GlnSer: 1.936 ± 0.287
1.999GlnThr: 1.999 ± 0.404
2.811GlnVal: 2.811 ± 0.476
0.75GlnTrp: 0.75 ± 0.196
0.812GlnTyr: 0.812 ± 0.182
0.0GlnXaa: 0.0 ± 0.0
Arg
5.622ArgAla: 5.622 ± 0.685
0.75ArgCys: 0.75 ± 0.222
2.873ArgAsp: 2.873 ± 0.39
4.747ArgGlu: 4.747 ± 0.726
1.936ArgPhe: 1.936 ± 0.364
5.497ArgGly: 5.497 ± 0.563
1.312ArgHis: 1.312 ± 0.281
2.811ArgIle: 2.811 ± 0.507
3.498ArgLys: 3.498 ± 0.48
5.31ArgLeu: 5.31 ± 0.676
1.562ArgMet: 1.562 ± 0.303
2.124ArgAsn: 2.124 ± 0.424
2.624ArgPro: 2.624 ± 0.5
1.999ArgGln: 1.999 ± 0.369
5.434ArgArg: 5.434 ± 0.618
3.436ArgSer: 3.436 ± 0.532
2.811ArgThr: 2.811 ± 0.479
5.185ArgVal: 5.185 ± 0.625
1.312ArgTrp: 1.312 ± 0.271
1.874ArgTyr: 1.874 ± 0.313
0.0ArgXaa: 0.0 ± 0.0
Ser
6.246SerAla: 6.246 ± 0.863
0.562SerCys: 0.562 ± 0.197
3.248SerAsp: 3.248 ± 0.494
4.123SerGlu: 4.123 ± 0.461
1.999SerPhe: 1.999 ± 0.512
6.184SerGly: 6.184 ± 0.81
1.562SerHis: 1.562 ± 0.299
2.686SerIle: 2.686 ± 0.467
2.436SerLys: 2.436 ± 0.393
4.747SerLeu: 4.747 ± 0.575
1.124SerMet: 1.124 ± 0.246
2.374SerAsn: 2.374 ± 0.431
3.123SerPro: 3.123 ± 0.478
1.936SerGln: 1.936 ± 0.325
3.248SerArg: 3.248 ± 0.396
3.81SerSer: 3.81 ± 0.719
3.373SerThr: 3.373 ± 0.485
3.998SerVal: 3.998 ± 0.531
1.374SerTrp: 1.374 ± 0.347
1.749SerTyr: 1.749 ± 0.342
0.0SerXaa: 0.0 ± 0.0
Thr
5.872ThrAla: 5.872 ± 0.882
0.375ThrCys: 0.375 ± 0.145
4.248ThrAsp: 4.248 ± 0.492
3.873ThrGlu: 3.873 ± 0.474
1.874ThrPhe: 1.874 ± 0.338
6.871ThrGly: 6.871 ± 0.69
0.999ThrHis: 0.999 ± 0.31
2.873ThrIle: 2.873 ± 0.601
2.561ThrLys: 2.561 ± 0.311
5.809ThrLeu: 5.809 ± 0.621
0.812ThrMet: 0.812 ± 0.263
1.811ThrAsn: 1.811 ± 0.403
3.748ThrPro: 3.748 ± 0.519
1.624ThrGln: 1.624 ± 0.301
3.311ThrArg: 3.311 ± 0.498
3.498ThrSer: 3.498 ± 0.562
4.185ThrThr: 4.185 ± 0.569
5.434ThrVal: 5.434 ± 0.74
0.937ThrTrp: 0.937 ± 0.24
2.061ThrTyr: 2.061 ± 0.337
0.0ThrXaa: 0.0 ± 0.0
Val
6.496ValAla: 6.496 ± 0.652
0.5ValCys: 0.5 ± 0.176
5.372ValAsp: 5.372 ± 0.642
4.747ValGlu: 4.747 ± 0.555
2.374ValPhe: 2.374 ± 0.339
5.185ValGly: 5.185 ± 0.769
1.562ValHis: 1.562 ± 0.233
3.998ValIle: 3.998 ± 0.504
2.624ValLys: 2.624 ± 0.391
5.247ValLeu: 5.247 ± 0.59
1.124ValMet: 1.124 ± 0.321
2.374ValAsn: 2.374 ± 0.357
4.123ValPro: 4.123 ± 0.517
2.249ValGln: 2.249 ± 0.377
5.122ValArg: 5.122 ± 0.728
5.559ValSer: 5.559 ± 0.527
5.684ValThr: 5.684 ± 0.674
4.997ValVal: 4.997 ± 0.677
1.312ValTrp: 1.312 ± 0.298
2.186ValTyr: 2.186 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
1.624TrpAla: 1.624 ± 0.348
0.125TrpCys: 0.125 ± 0.079
1.312TrpAsp: 1.312 ± 0.324
0.875TrpGlu: 0.875 ± 0.216
0.937TrpPhe: 0.937 ± 0.224
1.624TrpGly: 1.624 ± 0.309
0.375TrpHis: 0.375 ± 0.154
1.124TrpIle: 1.124 ± 0.232
0.375TrpLys: 0.375 ± 0.21
1.749TrpLeu: 1.749 ± 0.299
0.375TrpMet: 0.375 ± 0.174
0.625TrpAsn: 0.625 ± 0.248
0.937TrpPro: 0.937 ± 0.276
0.937TrpGln: 0.937 ± 0.223
1.124TrpArg: 1.124 ± 0.259
0.75TrpSer: 0.75 ± 0.225
1.562TrpThr: 1.562 ± 0.32
2.186TrpVal: 2.186 ± 0.336
0.5TrpTrp: 0.5 ± 0.203
0.312TrpTyr: 0.312 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.374TyrAla: 2.374 ± 0.399
0.25TyrCys: 0.25 ± 0.155
1.312TyrAsp: 1.312 ± 0.358
2.561TyrGlu: 2.561 ± 0.37
0.687TyrPhe: 0.687 ± 0.208
2.811TyrGly: 2.811 ± 0.427
0.625TyrHis: 0.625 ± 0.176
1.624TyrIle: 1.624 ± 0.351
1.124TyrLys: 1.124 ± 0.297
2.936TyrLeu: 2.936 ± 0.424
0.437TyrMet: 0.437 ± 0.157
1.312TyrAsn: 1.312 ± 0.293
1.374TyrPro: 1.374 ± 0.298
1.312TyrGln: 1.312 ± 0.3
2.436TyrArg: 2.436 ± 0.379
1.562TyrSer: 1.562 ± 0.3
2.249TyrThr: 2.249 ± 0.387
1.874TyrVal: 1.874 ± 0.309
0.312TyrTrp: 0.312 ± 0.171
0.812TyrTyr: 0.812 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (16010 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski