Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505)

Taxonomy: cellular organisms; Bacteria; FCB group; Gemmatimonadetes; Gemmatimonadetes; Gemmatimonadales; Gemmatimonadaceae; Gemmatimonas; Gemmatimonas aurantiaca

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C1AE61|C1AE61_GEMAT Putative fatty-acid desaturase OS=Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505) OX=379066 GN=GAU_3746 PE=3 SV=1
MM1 pKa = 7.68PCLPFEE7 pKa = 4.67MEE9 pKa = 4.14GAMQTMSVAEE19 pKa = 4.06QSRR22 pKa = 11.84LVGGDD27 pKa = 3.18FDD29 pKa = 4.78FGLFVRR35 pKa = 11.84GAACGLGMAAGVATAVGTVAAIAACLTLLPGEE67 pKa = 4.33LTT69 pKa = 3.66

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1AC18|C1AC18_GEMAT Uncharacterized protein OS=Gemmatimonas aurantiaca (strain T-27 / DSM 14586 / JCM 11422 / NBRC 100505) OX=379066 GN=GAU_3003 PE=4 SV=1
MM1 pKa = 7.54AARR4 pKa = 11.84RR5 pKa = 11.84MLAVLPVFIMLLVRR19 pKa = 11.84LWHH22 pKa = 6.24RR23 pKa = 11.84RR24 pKa = 11.84IGHH27 pKa = 6.28HH28 pKa = 6.41LVHH31 pKa = 6.75MMRR34 pKa = 11.84THH36 pKa = 5.58MRR38 pKa = 11.84MHH40 pKa = 5.79MRR42 pKa = 11.84MHH44 pKa = 6.24RR45 pKa = 11.84RR46 pKa = 11.84VRR48 pKa = 11.84MLVSVTARR56 pKa = 3.14

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3932

0

3932

1419465

38

2777

361.0

39.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.753 ± 0.051

0.692 ± 0.011

5.528 ± 0.027

5.022 ± 0.037

3.227 ± 0.021

8.466 ± 0.037

2.078 ± 0.02

4.234 ± 0.023

2.261 ± 0.035

9.916 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.017

2.468 ± 0.03

5.404 ± 0.028

3.458 ± 0.026

8.105 ± 0.04

5.878 ± 0.027

6.333 ± 0.032

8.36 ± 0.033

1.438 ± 0.017

2.11 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski