Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus; Zaire ebolavirus; Ebola virus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P60170|VSGP_EBOZM Pre-small/secreted glycoprotein OS=Zaire ebolavirus (strain Mayinga-76) OX=128952 GN=GP PE=1 SV=1
MM1 pKa = 8.24DD2 pKa = 5.39SRR4 pKa = 11.84PQKK7 pKa = 10.28IWMAPSLTEE16 pKa = 4.62SDD18 pKa = 3.12MDD20 pKa = 3.5YY21 pKa = 11.37HH22 pKa = 7.46KK23 pKa = 10.77ILTAGLSVQQGIVRR37 pKa = 11.84QRR39 pKa = 11.84VIPVYY44 pKa = 9.99QVNNLEE50 pKa = 4.85EE51 pKa = 4.26ICQLIIQAFEE61 pKa = 4.21AGVDD65 pKa = 4.26FQEE68 pKa = 4.79SADD71 pKa = 3.83SFLLMLCLHH80 pKa = 7.05HH81 pKa = 7.47AYY83 pKa = 10.35QGDD86 pKa = 3.72YY87 pKa = 11.27KK88 pKa = 11.19LFLEE92 pKa = 4.69SGAVKK97 pKa = 10.45YY98 pKa = 11.09LEE100 pKa = 3.87GHH102 pKa = 5.74GFRR105 pKa = 11.84FEE107 pKa = 4.07VKK109 pKa = 10.23KK110 pKa = 10.7RR111 pKa = 11.84DD112 pKa = 3.34GVKK115 pKa = 10.25RR116 pKa = 11.84LEE118 pKa = 4.21EE119 pKa = 3.99LLPAVSSGKK128 pKa = 10.01NIKK131 pKa = 8.74RR132 pKa = 11.84TLAAMPEE139 pKa = 4.26EE140 pKa = 4.61EE141 pKa = 4.34TTEE144 pKa = 4.02ANAGQFLSFASLFLPKK160 pKa = 10.39LVVGEE165 pKa = 4.31KK166 pKa = 10.46ACLEE170 pKa = 4.0KK171 pKa = 10.81VQRR174 pKa = 11.84QIQVHH179 pKa = 6.09AEE181 pKa = 3.78QGLIQYY187 pKa = 7.32PTAWQSVGHH196 pKa = 5.52MMVIFRR202 pKa = 11.84LMRR205 pKa = 11.84TNFLIKK211 pKa = 10.22FLLIHH216 pKa = 6.24QGMHH220 pKa = 5.09MVAGHH225 pKa = 7.11DD226 pKa = 3.84ANDD229 pKa = 3.63AVISNSVAQARR240 pKa = 11.84FSGLLIVKK248 pKa = 8.23TVLDD252 pKa = 4.78HH253 pKa = 7.38ILQKK257 pKa = 8.28TEE259 pKa = 3.67RR260 pKa = 11.84GVRR263 pKa = 11.84LHH265 pKa = 6.46PLARR269 pKa = 11.84TAKK272 pKa = 9.83VKK274 pKa = 11.22NEE276 pKa = 3.83VNSFKK281 pKa = 10.95AALSSLAKK289 pKa = 9.86HH290 pKa = 6.16GEE292 pKa = 3.99YY293 pKa = 10.9APFARR298 pKa = 11.84LLNLSGVNNLEE309 pKa = 4.48HH310 pKa = 7.06GLFPQLSAIALGVATAHH327 pKa = 6.58GSTLAGVNVGEE338 pKa = 4.64QYY340 pKa = 10.17QQLRR344 pKa = 11.84EE345 pKa = 4.06AATEE349 pKa = 4.1AEE351 pKa = 4.44KK352 pKa = 10.74QLQQYY357 pKa = 10.35AEE359 pKa = 4.16SRR361 pKa = 11.84EE362 pKa = 3.99LDD364 pKa = 3.55HH365 pKa = 7.43LGLDD369 pKa = 3.83DD370 pKa = 4.06QEE372 pKa = 5.56KK373 pKa = 9.82KK374 pKa = 10.65ILMNFHH380 pKa = 5.72QKK382 pKa = 10.38KK383 pKa = 10.33NEE385 pKa = 3.58ISFQQTNAMVTLRR398 pKa = 11.84KK399 pKa = 9.42EE400 pKa = 3.98RR401 pKa = 11.84LAKK404 pKa = 9.27LTEE407 pKa = 5.11AITAASLPKK416 pKa = 10.33TSGHH420 pKa = 6.29YY421 pKa = 10.7DD422 pKa = 3.75DD423 pKa = 6.46DD424 pKa = 6.63DD425 pKa = 6.0DD426 pKa = 5.55IPFPGPINDD435 pKa = 4.78DD436 pKa = 4.41DD437 pKa = 4.81NPGHH441 pKa = 6.58QDD443 pKa = 4.06DD444 pKa = 5.69DD445 pKa = 4.13PTDD448 pKa = 3.87SQDD451 pKa = 3.01TTIPDD456 pKa = 3.71VVVDD460 pKa = 4.75PDD462 pKa = 3.79DD463 pKa = 4.14GSYY466 pKa = 11.56GEE468 pKa = 4.07YY469 pKa = 10.17QSYY472 pKa = 10.58SEE474 pKa = 4.71NGMNAPDD481 pKa = 4.41DD482 pKa = 4.49LVLFDD487 pKa = 6.03LDD489 pKa = 5.11EE490 pKa = 6.61DD491 pKa = 4.98DD492 pKa = 5.69EE493 pKa = 4.56DD494 pKa = 4.23TKK496 pKa = 11.0PVPNRR501 pKa = 11.84STKK504 pKa = 10.49GGQQKK509 pKa = 10.34NSQKK513 pKa = 9.96GQHH516 pKa = 5.72IEE518 pKa = 3.92GRR520 pKa = 11.84QTQSRR525 pKa = 11.84PIQNVPGPHH534 pKa = 5.51RR535 pKa = 11.84TIHH538 pKa = 6.16HH539 pKa = 6.92ASAPLTDD546 pKa = 3.32NDD548 pKa = 4.4RR549 pKa = 11.84RR550 pKa = 11.84NEE552 pKa = 4.06PSGSTSPRR560 pKa = 11.84MLTPINEE567 pKa = 4.36EE568 pKa = 4.16ADD570 pKa = 3.9PLDD573 pKa = 4.54DD574 pKa = 6.08ADD576 pKa = 5.93DD577 pKa = 4.21EE578 pKa = 4.83TSSLPPLEE586 pKa = 5.6SDD588 pKa = 5.56DD589 pKa = 4.71EE590 pKa = 4.3EE591 pKa = 4.48QDD593 pKa = 3.9RR594 pKa = 11.84DD595 pKa = 3.69GTSNRR600 pKa = 11.84TPTVAPPAPVYY611 pKa = 10.21RR612 pKa = 11.84DD613 pKa = 3.24HH614 pKa = 7.49SEE616 pKa = 4.16KK617 pKa = 10.78KK618 pKa = 9.94EE619 pKa = 3.98LPQDD623 pKa = 3.52EE624 pKa = 4.87QQDD627 pKa = 3.52QDD629 pKa = 3.46HH630 pKa = 5.74TQEE633 pKa = 4.96ARR635 pKa = 11.84NQDD638 pKa = 3.18SDD640 pKa = 3.73NTQSEE645 pKa = 4.51HH646 pKa = 7.33SFEE649 pKa = 3.72EE650 pKa = 4.72MYY652 pKa = 10.9RR653 pKa = 11.84HH654 pKa = 6.72ILRR657 pKa = 11.84SQGPFDD663 pKa = 3.59AVLYY667 pKa = 8.01YY668 pKa = 11.21HH669 pKa = 6.23MMKK672 pKa = 10.56DD673 pKa = 3.48EE674 pKa = 4.43PVVFSTSDD682 pKa = 3.15GKK684 pKa = 11.22EE685 pKa = 3.56YY686 pKa = 10.03TYY688 pKa = 10.88PDD690 pKa = 3.91SLEE693 pKa = 4.06EE694 pKa = 4.86EE695 pKa = 4.48YY696 pKa = 10.61PPWLTEE702 pKa = 3.81KK703 pKa = 10.66EE704 pKa = 4.2AMNEE708 pKa = 3.64EE709 pKa = 4.19NRR711 pKa = 11.84FVTLDD716 pKa = 3.26GQQFYY721 pKa = 9.84WPVMNHH727 pKa = 5.74KK728 pKa = 9.96NKK730 pKa = 9.79FMAILQHH737 pKa = 5.92HH738 pKa = 5.25QQ739 pKa = 2.95

Molecular weight: 83.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q05323|VP30_EBOZM Transcriptional activator VP30 OS=Zaire ebolavirus (strain Mayinga-76) OX=128952 GN=VP30 PE=1 SV=2
MM1 pKa = 7.54AKK3 pKa = 9.15ATGRR7 pKa = 11.84YY8 pKa = 9.15NLISPKK14 pKa = 9.97KK15 pKa = 8.31DD16 pKa = 3.15LEE18 pKa = 4.48KK19 pKa = 11.0GVVLSDD25 pKa = 3.51LCNFLVSQTIQGWKK39 pKa = 9.83VYY41 pKa = 8.73WAGIEE46 pKa = 3.85FDD48 pKa = 3.84VTHH51 pKa = 6.93KK52 pKa = 10.92GMALLHH58 pKa = 5.94RR59 pKa = 11.84LKK61 pKa = 10.38TNDD64 pKa = 3.85FAPAWSMTRR73 pKa = 11.84NLFPHH78 pKa = 7.3LFQNPNSTIEE88 pKa = 4.11SPLWALRR95 pKa = 11.84VILAAGIQDD104 pKa = 3.69QLIDD108 pKa = 3.49QSLIEE113 pKa = 4.47PLAGALGLISDD124 pKa = 4.69WLLTTNTNHH133 pKa = 6.45FNMRR137 pKa = 11.84TQRR140 pKa = 11.84VKK142 pKa = 10.77EE143 pKa = 3.89QLSLKK148 pKa = 8.85MLSLIRR154 pKa = 11.84SNILKK159 pKa = 10.16FINKK163 pKa = 9.24LDD165 pKa = 3.88ALHH168 pKa = 5.76VVNYY172 pKa = 10.4NGLLSSIEE180 pKa = 4.19IGTQNHH186 pKa = 6.3TIIITRR192 pKa = 11.84TNMGFLVEE200 pKa = 4.29LQEE203 pKa = 4.39PDD205 pKa = 3.16KK206 pKa = 11.53SAMNRR211 pKa = 11.84MKK213 pKa = 10.51PGPAKK218 pKa = 10.49FSLLHH223 pKa = 6.32EE224 pKa = 4.63STLKK228 pKa = 11.0AFTQGSSTRR237 pKa = 11.84MQSLILEE244 pKa = 4.63FNSSLAII251 pKa = 4.01

Molecular weight: 28.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5494

251

2212

610.4

68.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.17 ± 0.375

1.638 ± 0.23

5.133 ± 0.484

5.533 ± 0.5

4.587 ± 0.397

5.679 ± 0.572

2.967 ± 0.37

5.952 ± 0.504

5.224 ± 0.201

10.084 ± 0.637

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.675 ± 0.276

4.605 ± 0.266

5.479 ± 0.625

5.042 ± 0.454

5.206 ± 0.29

7.663 ± 0.323

7.608 ± 0.644

5.388 ± 0.286

1.365 ± 0.2

3.003 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski