Bacillus solimangrovi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5LAZ9|A0A1E5LAZ9_9BACI Flagellar FliJ protein OS=Bacillus solimangrovi OX=1305675 GN=BFG57_06490 PE=3 SV=1
MM1 pKa = 7.54TIHH4 pKa = 6.35VVPSDD9 pKa = 3.64FATVQEE15 pKa = 5.04AIDD18 pKa = 4.6DD19 pKa = 4.14VGTMPGDD26 pKa = 4.32SIQILAGTTLKK37 pKa = 11.01NNAGFDD43 pKa = 3.47IEE45 pKa = 4.8AVGGLGTNVFDD56 pKa = 6.41GNICGNSSHH65 pKa = 6.76GGLCII70 pKa = 4.59

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5LDC4|A0A1E5LDC4_9BACI Beta_helix domain-containing protein OS=Bacillus solimangrovi OX=1305675 GN=BFG57_17115 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.29NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3530

0

3530

1021236

26

1685

289.3

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.346 ± 0.044

0.727 ± 0.015

5.244 ± 0.04

7.872 ± 0.054

4.49 ± 0.035

6.544 ± 0.04

2.099 ± 0.025

8.184 ± 0.046

6.664 ± 0.037

9.59 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.02

4.93 ± 0.036

3.339 ± 0.024

3.922 ± 0.033

3.988 ± 0.034

6.176 ± 0.035

5.468 ± 0.029

7.147 ± 0.036

0.954 ± 0.013

3.57 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski