Kamese virus
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R1J2|A0A0D3R1J2_9RHAB Matrix OS=Kamese virus OX=200402 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.63 LFINAGLTFNYY13 pKa = 9.75 YY14 pKa = 10.5 LPEE17 pKa = 4.29 LPKK20 pKa = 10.84 DD21 pKa = 3.64 VLHH24 pKa = 6.68 EE25 pKa = 4.93 IISIMITRR33 pKa = 11.84 CVTMSGFPEE42 pKa = 4.39 DD43 pKa = 3.4 LAAFAVNWTWEE54 pKa = 4.11 QTQLCYY60 pKa = 10.84 YY61 pKa = 10.53 DD62 pKa = 4.86 FDD64 pKa = 4.31 VSHH67 pKa = 7.09 GYY69 pKa = 9.62 SWIQDD74 pKa = 4.04 DD75 pKa = 4.51 IEE77 pKa = 4.83 LPGDD81 pKa = 3.52 IKK83 pKa = 11.05 SDD85 pKa = 3.51 QPVLKK90 pKa = 9.49 EE91 pKa = 3.59 YY92 pKa = 10.57 RR93 pKa = 11.84 IRR95 pKa = 11.84 TEE97 pKa = 3.77 FPIGIDD103 pKa = 3.16 RR104 pKa = 11.84 NTGEE108 pKa = 4.34 VEE110 pKa = 3.46 FFIYY114 pKa = 10.33 INTQINRR121 pKa = 11.84 DD122 pKa = 3.55 APHH125 pKa = 7.12 WSSVWWPKK133 pKa = 11.12 LMDD136 pKa = 4.61 PLANYY141 pKa = 9.42 LFRR144 pKa = 11.84 NPDD147 pKa = 3.26 QVASKK152 pKa = 11.1 YY153 pKa = 10.87 GFLHH157 pKa = 5.82 MLYY160 pKa = 9.32 TT161 pKa = 4.07
Molecular weight: 19.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.484
IPC2_protein 4.711
IPC_protein 4.609
Toseland 4.469
ProMoST 4.749
Dawson 4.584
Bjellqvist 4.736
Wikipedia 4.482
Rodwell 4.469
Grimsley 4.38
Solomon 4.584
Lehninger 4.546
Nozaki 4.698
DTASelect 4.902
Thurlkill 4.482
EMBOSS 4.507
Sillero 4.749
Patrickios 2.893
IPC_peptide 4.584
IPC2_peptide 4.736
IPC2.peptide.svr19 4.688
Protein with the highest isoelectric point:
>tr|A0A0D3R117|A0A0D3R117_9RHAB Nucleocapsid protein OS=Kamese virus OX=200402 PE=4 SV=1
MM1 pKa = 7.61 GFNFDD6 pKa = 3.38 VDD8 pKa = 3.8 VAKK11 pKa = 10.42 PIQGAFKK18 pKa = 10.71 NLWNDD23 pKa = 2.65 ITRR26 pKa = 11.84 FFEE29 pKa = 5.43 PFLTWISDD37 pKa = 3.07 IGKK40 pKa = 7.92 WALIILLVIVSIKK53 pKa = 10.14 ILIVIYY59 pKa = 10.5 KK60 pKa = 9.9 IGKK63 pKa = 8.32 CLWKK67 pKa = 10.68 SGLCLKK73 pKa = 10.54 RR74 pKa = 11.84 CFRR77 pKa = 11.84 RR78 pKa = 11.84 IKK80 pKa = 8.76 KK81 pKa = 8.08 TKK83 pKa = 8.85 IRR85 pKa = 11.84 KK86 pKa = 8.96 KK87 pKa = 10.27 VSLKK91 pKa = 10.49 LRR93 pKa = 11.84 SKK95 pKa = 10.45 RR96 pKa = 11.84 IKK98 pKa = 10.11 KK99 pKa = 10.03 KK100 pKa = 10.69 RR101 pKa = 11.84 ILL103 pKa = 3.67
Molecular weight: 12.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.245
IPC2_protein 9.867
IPC_protein 10.175
Toseland 11.155
ProMoST 10.701
Dawson 11.199
Bjellqvist 10.833
Wikipedia 11.345
Rodwell 11.711
Grimsley 11.213
Solomon 11.316
Lehninger 11.286
Nozaki 11.125
DTASelect 10.818
Thurlkill 11.125
EMBOSS 11.55
Sillero 11.125
Patrickios 11.433
IPC_peptide 11.33
IPC2_peptide 9.619
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
4319
100
2104
431.9
49.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.584 ± 0.677
1.806 ± 0.282
5.302 ± 0.516
5.858 ± 0.337
4.237 ± 0.327
5.302 ± 0.21
2.524 ± 0.291
7.293 ± 0.513
5.696 ± 0.747
10.28 ± 0.976
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.207
5.858 ± 0.538
4.538 ± 0.439
3.473 ± 0.294
5.487 ± 0.345
7.78 ± 0.476
5.464 ± 0.498
5.534 ± 0.251
2.269 ± 0.25
4.075 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here