Lactococcus phage 98104

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BM11|A0A1P8BM11_9CAUD Uncharacterized protein OS=Lactococcus phage 98104 OX=1868857 GN=DS98104_35 PE=4 SV=1
MM1 pKa = 7.13VKK3 pKa = 10.12KK4 pKa = 10.81LKK6 pKa = 9.95GAEE9 pKa = 3.84MSEE12 pKa = 4.26LEE14 pKa = 4.37KK15 pKa = 11.4NSDD18 pKa = 3.49TFVLFNSHH26 pKa = 5.0YY27 pKa = 10.17QVYY30 pKa = 9.7LAIGTGTLMIEE41 pKa = 4.28ANVVHH46 pKa = 6.04TTASPEE52 pKa = 3.94TARR55 pKa = 11.84TFSIYY60 pKa = 10.3AQARR64 pKa = 11.84EE65 pKa = 3.98FMNLLNEE72 pKa = 4.18YY73 pKa = 9.79HH74 pKa = 7.24DD75 pKa = 4.55KK76 pKa = 11.07EE77 pKa = 4.24FEE79 pKa = 3.71AWEE82 pKa = 4.48VINSEE87 pKa = 4.61YY88 pKa = 10.23IDD90 pKa = 4.0FFCKK94 pKa = 9.94VAEE97 pKa = 4.7PKK99 pKa = 10.14PQLTIPKK106 pKa = 9.61SIADD110 pKa = 3.69EE111 pKa = 4.22LEE113 pKa = 3.93EE114 pKa = 4.69EE115 pKa = 4.16IEE117 pKa = 4.03EE118 pKa = 4.87AYY120 pKa = 10.89SSDD123 pKa = 3.88YY124 pKa = 10.19IRR126 pKa = 11.84SYY128 pKa = 11.49SDD130 pKa = 2.59VGAYY134 pKa = 9.13MEE136 pKa = 5.06VITDD140 pKa = 3.84GLDD143 pKa = 3.41EE144 pKa = 4.37EE145 pKa = 4.75SEE147 pKa = 4.46LYY149 pKa = 10.93KK150 pKa = 11.2FMFPDD155 pKa = 4.72DD156 pKa = 4.24ALLGCSHH163 pKa = 7.0RR164 pKa = 11.84NIIYY168 pKa = 10.39LYY170 pKa = 10.78LVDD173 pKa = 4.28NDD175 pKa = 3.76LVKK178 pKa = 10.84VVEE181 pKa = 5.18GINDD185 pKa = 3.31NYY187 pKa = 11.45

Molecular weight: 21.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BLY0|A0A1P8BLY0_9CAUD Uncharacterized protein OS=Lactococcus phage 98104 OX=1868857 GN=DS98104_16 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.57YY3 pKa = 9.71IPKK6 pKa = 10.05YY7 pKa = 9.07SRR9 pKa = 11.84EE10 pKa = 4.05RR11 pKa = 11.84QKK13 pKa = 10.94KK14 pKa = 8.58RR15 pKa = 11.84RR16 pKa = 11.84NNDD19 pKa = 3.27VIRR22 pKa = 11.84VTTSPFLTEE31 pKa = 3.67VHH33 pKa = 6.7SIADD37 pKa = 3.34ATVNIVRR44 pKa = 11.84NEE46 pKa = 3.75IKK48 pKa = 10.3KK49 pKa = 10.67ANYY52 pKa = 9.71QIGKK56 pKa = 9.61

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10792

42

929

196.2

22.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.514 ± 0.386

0.63 ± 0.151

6.292 ± 0.156

7.496 ± 0.645

4.392 ± 0.223

6.31 ± 0.401

1.307 ± 0.135

6.672 ± 0.263

9.136 ± 0.417

7.932 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.147

6.375 ± 0.232

2.474 ± 0.168

4.476 ± 0.267

3.642 ± 0.322

6.607 ± 0.287

6.153 ± 0.296

5.94 ± 0.362

1.436 ± 0.185

3.632 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski