Alysiella crassa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Alysiella

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2612 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A376BK83|A0A376BK83_9NEIS Superfamily II helicase and inactivated derivatives OS=Alysiella crassa OX=153491 GN=NCTC10283_00096 PE=4 SV=1
MM1 pKa = 6.68NTVVNPNHH9 pKa = 6.34DD10 pKa = 3.99TANIAYY16 pKa = 8.88LQGAISALEE25 pKa = 4.02SVEE28 pKa = 4.06TLMSNSDD35 pKa = 3.7EE36 pKa = 4.44NLTLDD41 pKa = 5.02DD42 pKa = 4.09VFQFIQDD49 pKa = 3.53FKK51 pKa = 10.55IGLMEE56 pKa = 4.24EE57 pKa = 4.87CEE59 pKa = 4.49CKK61 pKa = 10.39QGVQSS66 pKa = 3.9

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A376BT56|A0A376BT56_9NEIS Uncharacterized protein OS=Alysiella crassa OX=153491 GN=NCTC10283_01680 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.13RR3 pKa = 11.84HH4 pKa = 5.49SVQLLALALSAAVASKK20 pKa = 9.97PVSIPTPKK28 pKa = 9.53FTQSVPLAHH37 pKa = 6.78AVIRR41 pKa = 11.84KK42 pKa = 8.39HH43 pKa = 5.23RR44 pKa = 11.84HH45 pKa = 4.75SGVAANRR52 pKa = 11.84RR53 pKa = 11.84MAKK56 pKa = 9.47KK57 pKa = 9.87RR58 pKa = 11.84KK59 pKa = 8.71KK60 pKa = 10.73AKK62 pKa = 8.87TT63 pKa = 3.37

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2612

0

2612

795213

31

7019

304.4

33.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.882 ± 0.093

0.983 ± 0.019

5.254 ± 0.049

5.823 ± 0.049

4.153 ± 0.047

6.679 ± 0.057

2.449 ± 0.03

5.989 ± 0.039

5.527 ± 0.049

10.018 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.034

4.807 ± 0.06

4.113 ± 0.054

5.102 ± 0.061

4.617 ± 0.057

5.442 ± 0.043

5.462 ± 0.105

6.767 ± 0.054

1.364 ± 0.025

3.002 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski