Salmonella virus VSiP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Cornellvirus; unclassified Cornellvirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385EF07|A0A385EF07_9CAUD Uncharacterized protein OS=Salmonella virus VSiP OX=2301721 GN=vsip_71 PE=4 SV=1
MM1 pKa = 7.41SEE3 pKa = 4.17WIKK6 pKa = 10.84CSEE9 pKa = 4.01QMPDD13 pKa = 2.99AFSDD17 pKa = 3.36VHH19 pKa = 7.94ISNGYY24 pKa = 9.96DD25 pKa = 3.12VGQGWWDD32 pKa = 3.26GGIWQTQHH40 pKa = 7.1DD41 pKa = 5.5YY42 pKa = 11.3YY43 pKa = 11.15SVPGEE48 pKa = 4.16VTHH51 pKa = 6.87WMPLPEE57 pKa = 5.33PPTEE61 pKa = 3.92

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385EED2|A0A385EED2_9CAUD Uncharacterized protein OS=Salmonella virus VSiP OX=2301721 GN=vsip_49 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LTPKK6 pKa = 10.29EE7 pKa = 3.79PEE9 pKa = 3.64PRR11 pKa = 11.84KK12 pKa = 10.15RR13 pKa = 11.84CIQCRR18 pKa = 11.84EE19 pKa = 4.06RR20 pKa = 11.84LPLSAFHH27 pKa = 6.84KK28 pKa = 10.58SSVASIDD35 pKa = 3.94DD36 pKa = 3.45YY37 pKa = 12.05RR38 pKa = 11.84NVCKK42 pKa = 10.27SCRR45 pKa = 11.84RR46 pKa = 11.84ATEE49 pKa = 3.53AARR52 pKa = 11.84IRR54 pKa = 11.84EE55 pKa = 4.17KK56 pKa = 10.84RR57 pKa = 11.84NKK59 pKa = 9.7

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13337

38

848

182.7

20.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.792 ± 0.478

1.282 ± 0.141

6.231 ± 0.23

6.576 ± 0.362

3.599 ± 0.156

7.648 ± 0.248

1.755 ± 0.181

5.196 ± 0.174

5.803 ± 0.302

7.49 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.15

4.536 ± 0.235

3.816 ± 0.181

3.996 ± 0.315

5.653 ± 0.228

5.908 ± 0.309

6.426 ± 0.471

6.883 ± 0.312

1.485 ± 0.149

3.397 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski