Amino acid dipepetide frequency for Arthrobacter phage Polka

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.904AlaAla: 21.904 ± 1.974
0.741AlaCys: 0.741 ± 0.271
7.823AlaAsp: 7.823 ± 0.772
7.493AlaGlu: 7.493 ± 0.64
3.211AlaPhe: 3.211 ± 0.64
15.893AlaGly: 15.893 ± 1.226
1.4AlaHis: 1.4 ± 0.284
4.447AlaIle: 4.447 ± 0.596
2.141AlaLys: 2.141 ± 0.548
11.117AlaLeu: 11.117 ± 1.002
2.882AlaMet: 2.882 ± 0.487
2.553AlaAsn: 2.553 ± 0.422
5.188AlaPro: 5.188 ± 0.789
3.047AlaGln: 3.047 ± 0.413
9.717AlaArg: 9.717 ± 1.154
6.505AlaSer: 6.505 ± 0.855
5.599AlaThr: 5.599 ± 0.725
11.364AlaVal: 11.364 ± 1.022
3.047AlaTrp: 3.047 ± 0.456
1.976AlaTyr: 1.976 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.247CysAla: 0.247 ± 0.144
0.0CysCys: 0.0 ± 0.0
0.576CysAsp: 0.576 ± 0.234
0.576CysGlu: 0.576 ± 0.177
0.0CysPhe: 0.0 ± 0.0
1.729CysGly: 1.729 ± 0.444
0.165CysHis: 0.165 ± 0.112
0.494CysIle: 0.494 ± 0.196
0.576CysLys: 0.576 ± 0.25
0.494CysLeu: 0.494 ± 0.195
0.082CysMet: 0.082 ± 0.088
0.082CysAsn: 0.082 ± 0.089
0.082CysPro: 0.082 ± 0.094
0.412CysGln: 0.412 ± 0.193
1.153CysArg: 1.153 ± 0.301
0.906CysSer: 0.906 ± 0.227
0.247CysThr: 0.247 ± 0.142
0.494CysVal: 0.494 ± 0.201
0.329CysTrp: 0.329 ± 0.17
0.082CysTyr: 0.082 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
5.682AspAla: 5.682 ± 0.658
0.494AspCys: 0.494 ± 0.187
2.223AspAsp: 2.223 ± 0.388
3.541AspGlu: 3.541 ± 0.672
1.647AspPhe: 1.647 ± 0.315
6.588AspGly: 6.588 ± 0.861
1.235AspHis: 1.235 ± 0.295
0.906AspIle: 0.906 ± 0.259
1.647AspLys: 1.647 ± 0.362
6.423AspLeu: 6.423 ± 0.743
0.576AspMet: 0.576 ± 0.235
0.988AspAsn: 0.988 ± 0.308
4.529AspPro: 4.529 ± 0.619
1.318AspGln: 1.318 ± 0.337
3.788AspArg: 3.788 ± 0.628
2.8AspSer: 2.8 ± 0.426
2.059AspThr: 2.059 ± 0.426
4.694AspVal: 4.694 ± 0.667
1.565AspTrp: 1.565 ± 0.306
1.565AspTyr: 1.565 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
6.67GluAla: 6.67 ± 0.752
0.741GluCys: 0.741 ± 0.228
3.706GluAsp: 3.706 ± 0.641
4.694GluGlu: 4.694 ± 0.683
1.647GluPhe: 1.647 ± 0.401
5.105GluGly: 5.105 ± 0.594
0.988GluHis: 0.988 ± 0.301
4.035GluIle: 4.035 ± 0.655
2.47GluLys: 2.47 ± 0.41
6.67GluLeu: 6.67 ± 0.711
0.906GluMet: 0.906 ± 0.308
1.729GluAsn: 1.729 ± 0.346
4.611GluPro: 4.611 ± 0.591
3.458GluGln: 3.458 ± 0.556
4.282GluArg: 4.282 ± 0.571
2.717GluSer: 2.717 ± 0.583
3.294GluThr: 3.294 ± 0.557
6.423GluVal: 6.423 ± 0.736
0.988GluTrp: 0.988 ± 0.272
1.235GluTyr: 1.235 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
2.717PheAla: 2.717 ± 0.387
0.082PheCys: 0.082 ± 0.085
2.223PheAsp: 2.223 ± 0.409
2.47PheGlu: 2.47 ± 0.432
0.576PhePhe: 0.576 ± 0.181
2.306PheGly: 2.306 ± 0.406
0.412PheHis: 0.412 ± 0.193
1.318PheIle: 1.318 ± 0.26
1.729PheLys: 1.729 ± 0.326
2.388PheLeu: 2.388 ± 0.5
0.741PheMet: 0.741 ± 0.217
0.988PheAsn: 0.988 ± 0.26
0.906PhePro: 0.906 ± 0.275
0.329PheGln: 0.329 ± 0.152
2.388PheArg: 2.388 ± 0.466
1.812PheSer: 1.812 ± 0.412
0.988PheThr: 0.988 ± 0.279
1.4PheVal: 1.4 ± 0.293
0.412PheTrp: 0.412 ± 0.156
0.659PheTyr: 0.659 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
10.952GlyAla: 10.952 ± 0.976
0.823GlyCys: 0.823 ± 0.252
5.352GlyAsp: 5.352 ± 0.722
5.682GlyGlu: 5.682 ± 0.628
3.211GlyPhe: 3.211 ± 0.568
8.482GlyGly: 8.482 ± 1.102
1.318GlyHis: 1.318 ± 0.348
3.706GlyIle: 3.706 ± 0.733
2.882GlyLys: 2.882 ± 0.541
7.74GlyLeu: 7.74 ± 1.136
2.717GlyMet: 2.717 ± 0.45
1.4GlyAsn: 1.4 ± 0.322
5.929GlyPro: 5.929 ± 0.694
2.635GlyGln: 2.635 ± 0.411
10.375GlyArg: 10.375 ± 0.83
5.27GlySer: 5.27 ± 0.94
5.435GlyThr: 5.435 ± 0.685
7.246GlyVal: 7.246 ± 1.071
2.882GlyTrp: 2.882 ± 0.503
2.306GlyTyr: 2.306 ± 0.36
0.0GlyXaa: 0.0 ± 0.0
His
1.565HisAla: 1.565 ± 0.346
0.165HisCys: 0.165 ± 0.119
0.741HisAsp: 0.741 ± 0.279
1.07HisGlu: 1.07 ± 0.386
0.412HisPhe: 0.412 ± 0.183
1.153HisGly: 1.153 ± 0.305
0.247HisHis: 0.247 ± 0.133
0.247HisIle: 0.247 ± 0.145
0.659HisLys: 0.659 ± 0.212
1.318HisLeu: 1.318 ± 0.397
0.247HisMet: 0.247 ± 0.145
0.165HisAsn: 0.165 ± 0.118
1.4HisPro: 1.4 ± 0.376
0.823HisGln: 0.823 ± 0.251
1.07HisArg: 1.07 ± 0.318
0.494HisSer: 0.494 ± 0.188
0.412HisThr: 0.412 ± 0.232
1.318HisVal: 1.318 ± 0.367
0.082HisTrp: 0.082 ± 0.084
0.741HisTyr: 0.741 ± 0.253
0.0HisXaa: 0.0 ± 0.0
Ile
4.529IleAla: 4.529 ± 0.571
0.247IleCys: 0.247 ± 0.16
2.141IleAsp: 2.141 ± 0.378
2.553IleGlu: 2.553 ± 0.423
0.576IlePhe: 0.576 ± 0.2
3.376IleGly: 3.376 ± 0.479
0.412IleHis: 0.412 ± 0.161
1.07IleIle: 1.07 ± 0.328
0.906IleLys: 0.906 ± 0.286
3.294IleLeu: 3.294 ± 0.492
0.906IleMet: 0.906 ± 0.28
1.07IleAsn: 1.07 ± 0.328
1.318IlePro: 1.318 ± 0.31
0.906IleGln: 0.906 ± 0.213
3.953IleArg: 3.953 ± 0.693
2.553IleSer: 2.553 ± 0.483
2.47IleThr: 2.47 ± 0.468
2.964IleVal: 2.964 ± 0.373
0.659IleTrp: 0.659 ± 0.177
0.165IleTyr: 0.165 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
5.023LysAla: 5.023 ± 0.702
0.247LysCys: 0.247 ± 0.146
1.647LysAsp: 1.647 ± 0.352
1.894LysGlu: 1.894 ± 0.441
1.07LysPhe: 1.07 ± 0.269
2.141LysGly: 2.141 ± 0.454
0.659LysHis: 0.659 ± 0.241
0.741LysIle: 0.741 ± 0.265
1.976LysLys: 1.976 ± 0.45
2.8LysLeu: 2.8 ± 0.494
1.318LysMet: 1.318 ± 0.393
1.153LysAsn: 1.153 ± 0.362
2.388LysPro: 2.388 ± 0.574
1.729LysGln: 1.729 ± 0.302
2.141LysArg: 2.141 ± 0.412
2.553LysSer: 2.553 ± 0.479
2.8LysThr: 2.8 ± 0.409
2.223LysVal: 2.223 ± 0.402
0.576LysTrp: 0.576 ± 0.231
0.659LysTyr: 0.659 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
11.364LeuAla: 11.364 ± 1.106
1.235LeuCys: 1.235 ± 0.352
4.776LeuAsp: 4.776 ± 0.708
8.976LeuGlu: 8.976 ± 0.931
2.059LeuPhe: 2.059 ± 0.391
8.976LeuGly: 8.976 ± 0.831
0.659LeuHis: 0.659 ± 0.285
3.211LeuIle: 3.211 ± 0.54
3.376LeuLys: 3.376 ± 0.532
7.658LeuLeu: 7.658 ± 1.087
1.812LeuMet: 1.812 ± 0.428
2.47LeuAsn: 2.47 ± 0.489
5.27LeuPro: 5.27 ± 0.562
2.8LeuGln: 2.8 ± 0.457
6.835LeuArg: 6.835 ± 0.803
4.364LeuSer: 4.364 ± 0.641
4.776LeuThr: 4.776 ± 0.803
7.164LeuVal: 7.164 ± 1.079
1.482LeuTrp: 1.482 ± 0.422
1.318LeuTyr: 1.318 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
2.388MetAla: 2.388 ± 0.438
0.247MetCys: 0.247 ± 0.13
1.4MetAsp: 1.4 ± 0.347
1.07MetGlu: 1.07 ± 0.315
0.165MetPhe: 0.165 ± 0.122
2.388MetGly: 2.388 ± 0.386
0.165MetHis: 0.165 ± 0.099
0.494MetIle: 0.494 ± 0.19
0.906MetLys: 0.906 ± 0.232
1.976MetLeu: 1.976 ± 0.372
0.576MetMet: 0.576 ± 0.196
0.412MetAsn: 0.412 ± 0.206
0.823MetPro: 0.823 ± 0.242
0.165MetGln: 0.165 ± 0.123
1.482MetArg: 1.482 ± 0.446
2.306MetSer: 2.306 ± 0.391
1.812MetThr: 1.812 ± 0.349
1.482MetVal: 1.482 ± 0.35
0.247MetTrp: 0.247 ± 0.133
0.165MetTyr: 0.165 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
2.47AsnAla: 2.47 ± 0.707
0.0AsnCys: 0.0 ± 0.0
1.07AsnAsp: 1.07 ± 0.221
1.4AsnGlu: 1.4 ± 0.334
0.823AsnPhe: 0.823 ± 0.368
2.8AsnGly: 2.8 ± 0.489
0.494AsnHis: 0.494 ± 0.282
0.988AsnIle: 0.988 ± 0.325
1.4AsnLys: 1.4 ± 0.403
1.894AsnLeu: 1.894 ± 0.301
0.659AsnMet: 0.659 ± 0.225
0.412AsnAsn: 0.412 ± 0.176
1.318AsnPro: 1.318 ± 0.268
0.823AsnGln: 0.823 ± 0.253
1.812AsnArg: 1.812 ± 0.356
0.988AsnSer: 0.988 ± 0.3
0.741AsnThr: 0.741 ± 0.234
2.141AsnVal: 2.141 ± 0.361
0.494AsnTrp: 0.494 ± 0.234
0.494AsnTyr: 0.494 ± 0.19
0.0AsnXaa: 0.0 ± 0.0
Pro
9.305ProAla: 9.305 ± 0.944
0.329ProCys: 0.329 ± 0.172
3.623ProAsp: 3.623 ± 0.59
5.27ProGlu: 5.27 ± 0.701
1.07ProPhe: 1.07 ± 0.34
5.27ProGly: 5.27 ± 0.712
0.988ProHis: 0.988 ± 0.276
1.647ProIle: 1.647 ± 0.34
1.812ProLys: 1.812 ± 0.394
3.788ProLeu: 3.788 ± 0.527
0.988ProMet: 0.988 ± 0.224
1.07ProAsn: 1.07 ± 0.265
2.964ProPro: 2.964 ± 0.652
1.153ProGln: 1.153 ± 0.374
3.294ProArg: 3.294 ± 0.501
2.882ProSer: 2.882 ± 0.486
1.976ProThr: 1.976 ± 0.417
4.776ProVal: 4.776 ± 0.684
1.812ProTrp: 1.812 ± 0.323
0.823ProTyr: 0.823 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
3.623GlnAla: 3.623 ± 0.628
0.082GlnCys: 0.082 ± 0.095
1.318GlnAsp: 1.318 ± 0.298
1.812GlnGlu: 1.812 ± 0.356
0.906GlnPhe: 0.906 ± 0.278
2.059GlnGly: 2.059 ± 0.345
0.988GlnHis: 0.988 ± 0.289
1.729GlnIle: 1.729 ± 0.317
0.906GlnLys: 0.906 ± 0.243
4.117GlnLeu: 4.117 ± 0.589
0.741GlnMet: 0.741 ± 0.229
1.07GlnAsn: 1.07 ± 0.289
1.729GlnPro: 1.729 ± 0.405
0.906GlnGln: 0.906 ± 0.297
1.812GlnArg: 1.812 ± 0.412
0.823GlnSer: 0.823 ± 0.201
1.4GlnThr: 1.4 ± 0.371
2.553GlnVal: 2.553 ± 0.629
0.494GlnTrp: 0.494 ± 0.173
0.659GlnTyr: 0.659 ± 0.234
0.0GlnXaa: 0.0 ± 0.0
Arg
11.034ArgAla: 11.034 ± 1.417
0.988ArgCys: 0.988 ± 0.309
3.87ArgAsp: 3.87 ± 0.664
3.953ArgGlu: 3.953 ± 0.609
2.388ArgPhe: 2.388 ± 0.418
6.341ArgGly: 6.341 ± 0.66
1.318ArgHis: 1.318 ± 0.38
2.8ArgIle: 2.8 ± 0.465
3.458ArgLys: 3.458 ± 0.514
6.588ArgLeu: 6.588 ± 0.817
1.07ArgMet: 1.07 ± 0.314
1.976ArgAsn: 1.976 ± 0.468
4.611ArgPro: 4.611 ± 0.776
3.294ArgGln: 3.294 ± 0.544
6.67ArgArg: 6.67 ± 0.938
4.447ArgSer: 4.447 ± 0.598
5.27ArgThr: 5.27 ± 0.672
6.835ArgVal: 6.835 ± 0.61
0.906ArgTrp: 0.906 ± 0.303
1.729ArgTyr: 1.729 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
6.917SerAla: 6.917 ± 0.765
0.329SerCys: 0.329 ± 0.149
2.306SerAsp: 2.306 ± 0.375
2.306SerGlu: 2.306 ± 0.351
1.729SerPhe: 1.729 ± 0.382
7.164SerGly: 7.164 ± 0.938
0.823SerHis: 0.823 ± 0.263
1.976SerIle: 1.976 ± 0.407
2.388SerLys: 2.388 ± 0.46
5.435SerLeu: 5.435 ± 0.689
1.565SerMet: 1.565 ± 0.325
1.07SerAsn: 1.07 ± 0.27
2.223SerPro: 2.223 ± 0.52
1.4SerGln: 1.4 ± 0.331
3.294SerArg: 3.294 ± 0.628
3.458SerSer: 3.458 ± 0.608
3.047SerThr: 3.047 ± 0.472
4.694SerVal: 4.694 ± 0.603
1.565SerTrp: 1.565 ± 0.301
0.823SerTyr: 0.823 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
7.576ThrAla: 7.576 ± 0.764
0.494ThrCys: 0.494 ± 0.247
2.717ThrAsp: 2.717 ± 0.53
2.8ThrGlu: 2.8 ± 0.508
1.894ThrPhe: 1.894 ± 0.492
5.023ThrGly: 5.023 ± 0.886
0.494ThrHis: 0.494 ± 0.192
2.882ThrIle: 2.882 ± 0.532
1.976ThrLys: 1.976 ± 0.408
4.611ThrLeu: 4.611 ± 0.473
0.823ThrMet: 0.823 ± 0.216
1.976ThrAsn: 1.976 ± 0.415
2.223ThrPro: 2.223 ± 0.429
1.07ThrGln: 1.07 ± 0.278
3.047ThrArg: 3.047 ± 0.549
2.223ThrSer: 2.223 ± 0.334
3.706ThrThr: 3.706 ± 0.697
5.682ThrVal: 5.682 ± 0.761
1.318ThrTrp: 1.318 ± 0.265
1.647ThrTyr: 1.647 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
9.881ValAla: 9.881 ± 1.012
0.906ValCys: 0.906 ± 0.355
3.953ValAsp: 3.953 ± 0.508
6.67ValGlu: 6.67 ± 0.603
1.976ValPhe: 1.976 ± 0.42
6.341ValGly: 6.341 ± 1.189
1.07ValHis: 1.07 ± 0.353
2.553ValIle: 2.553 ± 0.36
3.458ValLys: 3.458 ± 0.575
7.329ValLeu: 7.329 ± 0.889
1.153ValMet: 1.153 ± 0.293
1.318ValAsn: 1.318 ± 0.316
5.599ValPro: 5.599 ± 0.661
2.47ValGln: 2.47 ± 0.457
8.235ValArg: 8.235 ± 0.911
4.282ValSer: 4.282 ± 0.761
6.176ValThr: 6.176 ± 0.689
7.74ValVal: 7.74 ± 1.118
1.812ValTrp: 1.812 ± 0.405
1.976ValTyr: 1.976 ± 0.415
0.0ValXaa: 0.0 ± 0.0
Trp
2.388TrpAla: 2.388 ± 0.426
0.165TrpCys: 0.165 ± 0.105
1.235TrpAsp: 1.235 ± 0.379
0.659TrpGlu: 0.659 ± 0.208
0.988TrpPhe: 0.988 ± 0.33
1.482TrpGly: 1.482 ± 0.387
0.412TrpHis: 0.412 ± 0.163
0.659TrpIle: 0.659 ± 0.345
0.659TrpLys: 0.659 ± 0.215
2.059TrpLeu: 2.059 ± 0.425
0.247TrpMet: 0.247 ± 0.138
0.823TrpAsn: 0.823 ± 0.256
1.153TrpPro: 1.153 ± 0.369
0.823TrpGln: 0.823 ± 0.265
1.894TrpArg: 1.894 ± 0.429
1.4TrpSer: 1.4 ± 0.334
1.4TrpThr: 1.4 ± 0.276
2.141TrpVal: 2.141 ± 0.413
0.741TrpTrp: 0.741 ± 0.287
0.329TrpTyr: 0.329 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.812TyrAla: 1.812 ± 0.416
0.412TyrCys: 0.412 ± 0.185
1.565TyrAsp: 1.565 ± 0.296
1.482TyrGlu: 1.482 ± 0.398
0.494TyrPhe: 0.494 ± 0.188
1.729TyrGly: 1.729 ± 0.437
0.082TyrHis: 0.082 ± 0.085
0.165TyrIle: 0.165 ± 0.126
0.412TyrLys: 0.412 ± 0.159
2.882TyrLeu: 2.882 ± 0.458
0.576TyrMet: 0.576 ± 0.238
0.494TyrAsn: 0.494 ± 0.204
0.576TyrPro: 0.576 ± 0.204
0.165TyrGln: 0.165 ± 0.117
2.388TyrArg: 2.388 ± 0.39
1.729TyrSer: 1.729 ± 0.372
0.576TyrThr: 0.576 ± 0.264
1.565TyrVal: 1.565 ± 0.3
0.247TyrTrp: 0.247 ± 0.132
0.082TyrTyr: 0.082 ± 0.088
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (12145 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski