Arthrobacter sp. PAMC25564
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3600 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P7STE6|A0A4P7STE6_9MICC Endoribonuclease YbeY OS=Arthrobacter sp. PAMC25564 OX=2565366 GN=ybeY PE=3 SV=1
MM1 pKa = 7.35 TMRR4 pKa = 11.84 TGFTEE9 pKa = 6.3 GEE11 pKa = 4.51 VCWTDD16 pKa = 3.67 LQTGDD21 pKa = 3.35 VDD23 pKa = 3.72 AAKK26 pKa = 10.57 AFYY29 pKa = 10.35 AAVFGWRR36 pKa = 11.84 YY37 pKa = 9.9 EE38 pKa = 4.1 DD39 pKa = 5.31 LPTPDD44 pKa = 3.16 GRR46 pKa = 11.84 SYY48 pKa = 11.58 AQAFLGDD55 pKa = 3.72 GLVTVIAPQPPQQEE69 pKa = 4.01 AAAGRR74 pKa = 11.84 WNVYY78 pKa = 9.39 FATDD82 pKa = 3.7 DD83 pKa = 3.73 ARR85 pKa = 11.84 EE86 pKa = 3.94 LAAEE90 pKa = 4.51 LAHH93 pKa = 6.06 STGTLEE99 pKa = 4.78 SGPEE103 pKa = 4.11 SVGGAGVMVFFAPPGGGSTGAWQAGSHH130 pKa = 6.5 FGAARR135 pKa = 11.84 IQEE138 pKa = 4.31 PGALAWAEE146 pKa = 4.13 LLTPEE151 pKa = 4.16 PQAAVGCFQQLFGHH165 pKa = 6.44 EE166 pKa = 4.27 VTEE169 pKa = 4.25 YY170 pKa = 10.09 PQDD173 pKa = 3.98 DD174 pKa = 3.98 GGTYY178 pKa = 7.37 TTLTVNGTEE187 pKa = 4.33 VAGIAPLPADD197 pKa = 4.88 DD198 pKa = 5.06 GGTRR202 pKa = 11.84 QAGWQVYY209 pKa = 9.43 FGVPNVADD217 pKa = 3.48 AVEE220 pKa = 4.08 AAVAAGAVVLVAPEE234 pKa = 4.06 DD235 pKa = 3.81 AGDD238 pKa = 4.0 AGALATLQDD247 pKa = 3.67 PQGGVFSLVEE257 pKa = 3.92 VV258 pKa = 3.9
Molecular weight: 26.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.63
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A4P7STK5|A0A4P7STK5_9MICC GntR family transcriptional regulator OS=Arthrobacter sp. PAMC25564 OX=2565366 GN=E5206_12460 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3600
0
3600
1191758
32
1728
331.0
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.273 ± 0.063
0.624 ± 0.009
5.511 ± 0.032
5.481 ± 0.039
3.259 ± 0.023
9.313 ± 0.032
2.093 ± 0.018
4.47 ± 0.028
2.791 ± 0.03
10.306 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.993 ± 0.016
2.422 ± 0.022
5.485 ± 0.031
3.063 ± 0.021
6.624 ± 0.036
5.804 ± 0.029
5.759 ± 0.032
8.24 ± 0.032
1.402 ± 0.017
2.086 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here