Microbacterium saperdae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543BMZ2|A0A543BMZ2_9MICO Acyl-CoA thioesterase-2 OS=Microbacterium saperdae OX=69368 GN=FB560_1853 PE=3 SV=1
MM1 pKa = 7.56LALTAGGGAAWAADD15 pKa = 3.66RR16 pKa = 11.84FLIEE20 pKa = 4.77HH21 pKa = 6.11VSITDD26 pKa = 3.17VSAYY30 pKa = 9.22EE31 pKa = 4.02AANSNTDD38 pKa = 3.57DD39 pKa = 3.76AVTEE43 pKa = 4.46TTHH46 pKa = 6.37ATVTDD51 pKa = 3.62TSYY54 pKa = 11.82DD55 pKa = 3.4SDD57 pKa = 3.64QADD60 pKa = 3.08ISISTVSTGSGDD72 pKa = 3.66DD73 pKa = 3.24TVTYY77 pKa = 10.12YY78 pKa = 11.05VADD81 pKa = 3.68VTLTDD86 pKa = 3.48ATALRR91 pKa = 11.84SAFAGDD97 pKa = 3.42AFGTNITEE105 pKa = 4.24TTSAIAAANDD115 pKa = 3.27AVFAINGDD123 pKa = 3.82YY124 pKa = 11.18YY125 pKa = 11.16GFRR128 pKa = 11.84DD129 pKa = 3.52TGIVIRR135 pKa = 11.84NGVAYY140 pKa = 9.73RR141 pKa = 11.84DD142 pKa = 3.32AGARR146 pKa = 11.84EE147 pKa = 4.06GLAFYY152 pKa = 10.75QDD154 pKa = 3.69GHH156 pKa = 7.51VEE158 pKa = 4.48LYY160 pKa = 11.05DD161 pKa = 3.71EE162 pKa = 4.67TATTAASLVADD173 pKa = 4.55GVWNTLSFGPALVEE187 pKa = 4.12DD188 pKa = 4.77SIVVDD193 pKa = 4.98GIEE196 pKa = 4.24EE197 pKa = 4.28IEE199 pKa = 3.99VDD201 pKa = 3.95TNFGNHH207 pKa = 6.69SIQGEE212 pKa = 4.24QPRR215 pKa = 11.84TAVGVIDD222 pKa = 4.18EE223 pKa = 4.26NHH225 pKa = 6.03LVFVVVDD232 pKa = 4.11GRR234 pKa = 11.84SAGYY238 pKa = 10.01SRR240 pKa = 11.84GVTMTEE246 pKa = 4.0LADD249 pKa = 3.45IMISLGAEE257 pKa = 3.59TAYY260 pKa = 11.22NLDD263 pKa = 3.93GGGSSTMYY271 pKa = 10.7FDD273 pKa = 5.04GEE275 pKa = 4.62LVNSPLGKK283 pKa = 9.81DD284 pKa = 3.42QEE286 pKa = 4.51RR287 pKa = 11.84ATSDD291 pKa = 2.72ILYY294 pKa = 10.05IAGSGQQ300 pKa = 3.03

Molecular weight: 31.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543BAH2|A0A543BAH2_9MICO Putative peptidoglycan lipid II flippase OS=Microbacterium saperdae OX=69368 GN=FB560_3294 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4035

0

4035

1318807

29

2025

326.8

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.457 ± 0.056

0.455 ± 0.008

6.257 ± 0.037

5.603 ± 0.036

3.181 ± 0.023

8.845 ± 0.03

1.961 ± 0.02

4.953 ± 0.027

1.847 ± 0.027

10.186 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.809 ± 0.015

1.946 ± 0.021

5.276 ± 0.027

2.816 ± 0.019

7.104 ± 0.045

5.785 ± 0.028

6.226 ± 0.039

8.82 ± 0.035

1.529 ± 0.015

1.945 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski