Salmonella phage smaug

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Epseptimavirus; unclassified Epseptimavirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8RHV3|A0A6G8RHV3_9CAUD D14 protein OS=Salmonella phage smaug OX=2713322 GN=smaug_45 PE=4 SV=1
MM1 pKa = 6.69HH2 pKa = 6.9QKK4 pKa = 10.65SIGDD8 pKa = 3.8LLVWPDD14 pKa = 3.66GSWCYY19 pKa = 10.37RR20 pKa = 11.84FEE22 pKa = 4.11FATDD26 pKa = 3.51DD27 pKa = 4.19FSWKK31 pKa = 10.3SDD33 pKa = 3.8DD34 pKa = 3.53FCVIYY39 pKa = 10.57EE40 pKa = 4.47DD41 pKa = 3.45TAEE44 pKa = 4.04WKK46 pKa = 10.46EE47 pKa = 3.71FLIKK51 pKa = 10.38EE52 pKa = 4.3GEE54 pKa = 4.15YY55 pKa = 11.2GEE57 pKa = 4.86EE58 pKa = 4.25DD59 pKa = 3.53FF60 pKa = 6.4

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8RHS6|A0A6G8RHS6_9CAUD Uncharacterized protein OS=Salmonella phage smaug OX=2713322 GN=smaug_6 PE=4 SV=1
MM1 pKa = 7.47GRR3 pKa = 11.84QKK5 pKa = 10.4LTIKK9 pKa = 10.44DD10 pKa = 3.39INIRR14 pKa = 11.84LADD17 pKa = 3.91RR18 pKa = 11.84GIQIVGEE25 pKa = 4.14YY26 pKa = 10.23VNQRR30 pKa = 11.84TKK32 pKa = 10.58IVFKK36 pKa = 9.94CQRR39 pKa = 11.84AHH41 pKa = 4.53VWW43 pKa = 3.04

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

158

0

158

32130

31

1226

203.4

22.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.663 ± 0.409

1.027 ± 0.093

6.029 ± 0.171

6.919 ± 0.215

4.192 ± 0.15

6.58 ± 0.223

1.861 ± 0.14

6.67 ± 0.171

7.186 ± 0.191

8.254 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.149

5.347 ± 0.175

3.567 ± 0.152

3.682 ± 0.202

4.594 ± 0.147

6.209 ± 0.153

5.764 ± 0.172

6.545 ± 0.152

1.341 ± 0.092

3.943 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski