Drosophila rhopaloa (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora;

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21090 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P4FGS5|A0A6P4FGS5_DRORH triple functional domain protein-like OS=Drosophila rhopaloa OX=1041015 GN=LOC108051042 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84PITVNFLLVALCVSSTIWPASGLQCYY28 pKa = 9.75NCVGEE33 pKa = 4.26EE34 pKa = 4.15CHH36 pKa = 6.87AEE38 pKa = 4.07TVPEE42 pKa = 4.38ISCNLDD48 pKa = 3.17DD49 pKa = 5.76SEE51 pKa = 6.03AFVLDD56 pKa = 3.19TSGILNRR63 pKa = 11.84FRR65 pKa = 11.84RR66 pKa = 11.84SPLVVKK72 pKa = 10.41PRR74 pKa = 11.84DD75 pKa = 3.75TEE77 pKa = 4.09NLDD80 pKa = 3.31EE81 pKa = 4.48STTDD85 pKa = 3.27STVTSTDD92 pKa = 2.88ASSASTTDD100 pKa = 2.77SDD102 pKa = 4.0VTSDD106 pKa = 3.35STIASTEE113 pKa = 4.04SSSATDD119 pKa = 3.14SSTDD123 pKa = 3.06SSTNPQTVGSTEE135 pKa = 4.07SSTDD139 pKa = 2.8SSTISSTDD147 pKa = 2.82SSTATTDD154 pKa = 3.52DD155 pKa = 3.96SSTSDD160 pKa = 3.27STEE163 pKa = 3.6SSTTSEE169 pKa = 4.82PGSSSPSTTASTTDD183 pKa = 3.23STSEE187 pKa = 4.02STTDD191 pKa = 3.01STTDD195 pKa = 2.97STTDD199 pKa = 2.97STTDD203 pKa = 2.97STTDD207 pKa = 2.97STTDD211 pKa = 2.94STTDD215 pKa = 3.22STSDD219 pKa = 3.18STTDD223 pKa = 3.25STSDD227 pKa = 3.19STTDD231 pKa = 2.96STTDD235 pKa = 3.08STSQSTSSSTSSSTSDD251 pKa = 2.97STTDD255 pKa = 3.21STPNSSPVTTISPPISYY272 pKa = 10.47KK273 pKa = 10.63LAACYY278 pKa = 10.22SIYY281 pKa = 10.6KK282 pKa = 10.1EE283 pKa = 4.07GEE285 pKa = 4.01LYY287 pKa = 10.41RR288 pKa = 11.84GCIKK292 pKa = 10.76VPTNEE297 pKa = 4.32SACEE301 pKa = 3.7AVKK304 pKa = 10.81DD305 pKa = 3.83KK306 pKa = 11.88LEE308 pKa = 4.03ITEE311 pKa = 5.37DD312 pKa = 3.75SADD315 pKa = 3.4QCDD318 pKa = 3.05ICLTDD323 pKa = 3.55KK324 pKa = 11.48CNGSTSLQVSLGAMILLLAMGLKK347 pKa = 10.41NLFF350 pKa = 3.81

Molecular weight: 36.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P4FLZ7|A0A6P4FLZ7_DRORH cilia- and flagella-associated protein 20 OS=Drosophila rhopaloa OX=1041015 GN=LOC108052803 PE=4 SV=1
MM1 pKa = 7.62AAHH4 pKa = 6.95KK5 pKa = 10.42SFRR8 pKa = 11.84IKK10 pKa = 10.51QKK12 pKa = 10.12LAKK15 pKa = 9.77KK16 pKa = 9.79LKK18 pKa = 9.05QNRR21 pKa = 11.84SVPQWVRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15937

5153

21090

13421361

32

21286

636.4

70.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.353 ± 0.022

2.055 ± 0.073

5.242 ± 0.015

6.534 ± 0.024

3.374 ± 0.016

6.254 ± 0.023

2.612 ± 0.012

4.815 ± 0.016

5.487 ± 0.025

8.887 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.013

4.732 ± 0.017

5.763 ± 0.037

5.404 ± 0.027

5.53 ± 0.017

8.416 ± 0.03

5.705 ± 0.02

5.867 ± 0.012

0.942 ± 0.007

2.806 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski