Indian cassava mosaic virus (ICMV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q08594|MVP_ICMV Movement protein BC1 OS=Indian cassava mosaic virus OX=31600 GN=BC1 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.55FPEE11 pKa = 4.6SVHH14 pKa = 6.55GFRR17 pKa = 11.84CMLAVKK23 pKa = 9.73YY24 pKa = 9.89LQLVEE29 pKa = 4.46CTYY32 pKa = 11.52SPDD35 pKa = 3.3TLGYY39 pKa = 10.88DD40 pKa = 4.48LIRR43 pKa = 11.84DD44 pKa = 4.38LFSVIRR50 pKa = 11.84AKK52 pKa = 10.94NYY54 pKa = 9.48VEE56 pKa = 3.83ATSRR60 pKa = 11.84YY61 pKa = 8.04HH62 pKa = 6.22NFYY65 pKa = 11.02SRR67 pKa = 11.84LEE69 pKa = 4.15GSSPSEE75 pKa = 3.65LRR77 pKa = 11.84QPIQQPCGCPYY88 pKa = 10.55CPRR91 pKa = 11.84HH92 pKa = 6.39KK93 pKa = 10.11KK94 pKa = 8.95TILDD98 pKa = 3.84KK99 pKa = 10.23QTHH102 pKa = 4.38QSEE105 pKa = 4.45AQVVSDD111 pKa = 3.46VV112 pKa = 3.42

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q08588|AC4_ICMV Protein AC4 OS=Indian cassava mosaic virus OX=31600 GN=AC4 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.95IIISTPGSKK16 pKa = 9.66VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.07SPYY27 pKa = 10.0SSRR30 pKa = 11.84AAVPTVRR37 pKa = 11.84VTKK40 pKa = 10.21RR41 pKa = 11.84QSWTNRR47 pKa = 11.84PINRR51 pKa = 11.84KK52 pKa = 7.59PRR54 pKa = 11.84WYY56 pKa = 10.62RR57 pKa = 11.84MYY59 pKa = 10.68RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.05VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.6VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.67RR104 pKa = 11.84VGKK107 pKa = 9.73RR108 pKa = 11.84FCVKK112 pKa = 10.3SIYY115 pKa = 10.1ILGKK119 pKa = 9.09IWMDD123 pKa = 3.37EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 9.9EE195 pKa = 3.88QALVRR200 pKa = 11.84RR201 pKa = 11.84FFRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.67FYY251 pKa = 11.26DD252 pKa = 3.51SVSNN256 pKa = 3.9

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1824

102

351

202.7

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.605 ± 0.416

2.193 ± 0.239

5.592 ± 0.2

4.002 ± 0.468

3.947 ± 0.378

5.537 ± 0.536

3.673 ± 0.415

6.031 ± 0.603

5.373 ± 0.519

7.456 ± 0.701

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.305

5.921 ± 0.422

5.976 ± 0.537

4.002 ± 0.531

7.511 ± 0.7

8.498 ± 0.667

5.757 ± 0.582

6.031 ± 0.884

1.645 ± 0.237

4.167 ± 0.571
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski