Amino acid dipepetide frequency for Escherichia phage ADB-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.467AlaAla: 8.467 ± 1.223
0.98AlaCys: 0.98 ± 0.326
3.919AlaAsp: 3.919 ± 0.539
5.668AlaGlu: 5.668 ± 0.762
2.239AlaPhe: 2.239 ± 0.388
5.878AlaGly: 5.878 ± 0.728
0.84AlaHis: 0.84 ± 0.205
6.578AlaIle: 6.578 ± 0.589
6.927AlaLys: 6.927 ± 0.997
6.158AlaLeu: 6.158 ± 0.709
3.079AlaMet: 3.079 ± 0.428
3.219AlaAsn: 3.219 ± 0.535
1.469AlaPro: 1.469 ± 0.285
3.779AlaGln: 3.779 ± 0.626
3.989AlaArg: 3.989 ± 0.573
5.528AlaSer: 5.528 ± 1.05
4.618AlaThr: 4.618 ± 0.724
5.178AlaVal: 5.178 ± 0.679
1.19AlaTrp: 1.19 ± 0.252
2.799AlaTyr: 2.799 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.63CysAla: 0.63 ± 0.224
0.28CysCys: 0.28 ± 0.149
1.19CysAsp: 1.19 ± 0.317
0.84CysGlu: 0.84 ± 0.227
0.56CysPhe: 0.56 ± 0.246
1.05CysGly: 1.05 ± 0.349
0.21CysHis: 0.21 ± 0.121
0.84CysIle: 0.84 ± 0.241
0.91CysLys: 0.91 ± 0.263
0.56CysLeu: 0.56 ± 0.249
0.21CysMet: 0.21 ± 0.132
0.98CysAsn: 0.98 ± 0.246
0.56CysPro: 0.56 ± 0.207
0.42CysGln: 0.42 ± 0.169
0.56CysArg: 0.56 ± 0.209
0.7CysSer: 0.7 ± 0.213
0.77CysThr: 0.77 ± 0.312
0.77CysVal: 0.77 ± 0.204
0.28CysTrp: 0.28 ± 0.143
0.49CysTyr: 0.49 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
6.648AspAla: 6.648 ± 0.811
0.49AspCys: 0.49 ± 0.216
4.058AspAsp: 4.058 ± 0.689
4.758AspGlu: 4.758 ± 0.551
3.009AspPhe: 3.009 ± 0.444
6.508AspGly: 6.508 ± 0.852
0.91AspHis: 0.91 ± 0.267
3.989AspIle: 3.989 ± 0.481
4.338AspLys: 4.338 ± 0.508
3.849AspLeu: 3.849 ± 0.637
1.609AspMet: 1.609 ± 0.368
3.429AspAsn: 3.429 ± 0.546
1.819AspPro: 1.819 ± 0.304
2.029AspGln: 2.029 ± 0.448
2.449AspArg: 2.449 ± 0.495
3.779AspSer: 3.779 ± 0.525
2.659AspThr: 2.659 ± 0.512
4.758AspVal: 4.758 ± 0.649
0.7AspTrp: 0.7 ± 0.217
2.659AspTyr: 2.659 ± 0.47
0.0AspXaa: 0.0 ± 0.0
Glu
5.388GluAla: 5.388 ± 0.669
0.91GluCys: 0.91 ± 0.309
4.198GluAsp: 4.198 ± 0.779
5.178GluGlu: 5.178 ± 0.771
3.359GluPhe: 3.359 ± 0.636
2.659GluGly: 2.659 ± 0.453
1.19GluHis: 1.19 ± 0.301
6.787GluIle: 6.787 ± 0.808
4.758GluLys: 4.758 ± 0.727
5.038GluLeu: 5.038 ± 0.693
2.309GluMet: 2.309 ± 0.356
2.939GluAsn: 2.939 ± 0.438
1.889GluPro: 1.889 ± 0.462
2.519GluGln: 2.519 ± 0.456
3.009GluArg: 3.009 ± 0.576
3.919GluSer: 3.919 ± 0.52
3.919GluThr: 3.919 ± 0.563
5.178GluVal: 5.178 ± 0.622
0.77GluTrp: 0.77 ± 0.239
2.659GluTyr: 2.659 ± 0.348
0.0GluXaa: 0.0 ± 0.0
Phe
2.869PheAla: 2.869 ± 0.501
0.7PheCys: 0.7 ± 0.244
2.939PheAsp: 2.939 ± 0.42
3.499PheGlu: 3.499 ± 0.538
1.539PhePhe: 1.539 ± 0.422
3.639PheGly: 3.639 ± 0.552
0.7PheHis: 0.7 ± 0.222
2.449PheIle: 2.449 ± 0.394
3.079PheLys: 3.079 ± 0.455
1.679PheLeu: 1.679 ± 0.299
1.679PheMet: 1.679 ± 0.339
2.239PheAsn: 2.239 ± 0.411
1.33PhePro: 1.33 ± 0.375
0.98PheGln: 0.98 ± 0.32
1.749PheArg: 1.749 ± 0.255
2.309PheSer: 2.309 ± 0.385
3.009PheThr: 3.009 ± 0.46
1.889PheVal: 1.889 ± 0.326
0.84PheTrp: 0.84 ± 0.23
0.91PheTyr: 0.91 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
4.968GlyAla: 4.968 ± 0.803
1.399GlyCys: 1.399 ± 0.318
5.038GlyAsp: 5.038 ± 0.62
4.758GlyGlu: 4.758 ± 0.488
2.729GlyPhe: 2.729 ± 0.396
5.668GlyGly: 5.668 ± 0.85
1.19GlyHis: 1.19 ± 0.392
3.779GlyIle: 3.779 ± 0.427
5.948GlyLys: 5.948 ± 0.54
4.338GlyLeu: 4.338 ± 0.472
2.589GlyMet: 2.589 ± 0.547
3.359GlyAsn: 3.359 ± 0.547
0.42GlyPro: 0.42 ± 0.163
2.099GlyGln: 2.099 ± 0.399
3.359GlyArg: 3.359 ± 0.358
5.598GlySer: 5.598 ± 0.62
3.779GlyThr: 3.779 ± 0.526
5.458GlyVal: 5.458 ± 0.602
1.679GlyTrp: 1.679 ± 0.279
3.079GlyTyr: 3.079 ± 0.594
0.0GlyXaa: 0.0 ± 0.0
His
1.12HisAla: 1.12 ± 0.302
0.42HisCys: 0.42 ± 0.197
1.19HisAsp: 1.19 ± 0.308
1.469HisGlu: 1.469 ± 0.321
0.7HisPhe: 0.7 ± 0.221
1.12HisGly: 1.12 ± 0.289
0.56HisHis: 0.56 ± 0.192
1.19HisIle: 1.19 ± 0.369
1.33HisLys: 1.33 ± 0.349
1.26HisLeu: 1.26 ± 0.334
0.49HisMet: 0.49 ± 0.168
0.77HisAsn: 0.77 ± 0.21
0.63HisPro: 0.63 ± 0.203
0.42HisGln: 0.42 ± 0.173
1.12HisArg: 1.12 ± 0.291
1.19HisSer: 1.19 ± 0.339
0.98HisThr: 0.98 ± 0.278
0.91HisVal: 0.91 ± 0.327
0.28HisTrp: 0.28 ± 0.134
0.84HisTyr: 0.84 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
6.088IleAla: 6.088 ± 0.756
0.77IleCys: 0.77 ± 0.219
5.598IleAsp: 5.598 ± 0.597
4.478IleGlu: 4.478 ± 0.531
2.729IlePhe: 2.729 ± 0.513
5.178IleGly: 5.178 ± 0.651
0.84IleHis: 0.84 ± 0.282
4.128IleIle: 4.128 ± 0.468
4.618IleLys: 4.618 ± 0.577
3.289IleLeu: 3.289 ± 0.682
1.819IleMet: 1.819 ± 0.366
4.128IleAsn: 4.128 ± 0.616
2.379IlePro: 2.379 ± 0.412
2.379IleGln: 2.379 ± 0.375
3.009IleArg: 3.009 ± 0.384
4.338IleSer: 4.338 ± 0.455
5.108IleThr: 5.108 ± 0.507
3.849IleVal: 3.849 ± 0.593
0.77IleTrp: 0.77 ± 0.231
2.239IleTyr: 2.239 ± 0.359
0.0IleXaa: 0.0 ± 0.0
Lys
6.368LysAla: 6.368 ± 1.025
0.84LysCys: 0.84 ± 0.283
4.198LysAsp: 4.198 ± 0.588
5.878LysGlu: 5.878 ± 0.572
2.939LysPhe: 2.939 ± 0.645
4.408LysGly: 4.408 ± 0.48
1.819LysHis: 1.819 ± 0.357
4.898LysIle: 4.898 ± 0.829
4.618LysLys: 4.618 ± 0.583
5.178LysLeu: 5.178 ± 0.625
3.009LysMet: 3.009 ± 0.546
2.939LysAsn: 2.939 ± 0.422
2.449LysPro: 2.449 ± 0.454
3.009LysGln: 3.009 ± 0.562
3.289LysArg: 3.289 ± 0.489
4.058LysSer: 4.058 ± 0.512
3.849LysThr: 3.849 ± 0.435
4.618LysVal: 4.618 ± 0.582
0.84LysTrp: 0.84 ± 0.283
2.239LysTyr: 2.239 ± 0.33
0.0LysXaa: 0.0 ± 0.0
Leu
5.038LeuAla: 5.038 ± 0.736
0.77LeuCys: 0.77 ± 0.216
4.198LeuAsp: 4.198 ± 0.502
3.429LeuGlu: 3.429 ± 0.523
1.819LeuPhe: 1.819 ± 0.352
3.639LeuGly: 3.639 ± 0.529
1.399LeuHis: 1.399 ± 0.348
4.478LeuIle: 4.478 ± 0.584
5.318LeuLys: 5.318 ± 0.809
4.548LeuLeu: 4.548 ± 0.543
1.539LeuMet: 1.539 ± 0.324
2.729LeuAsn: 2.729 ± 0.493
3.079LeuPro: 3.079 ± 0.485
1.819LeuGln: 1.819 ± 0.365
3.569LeuArg: 3.569 ± 0.423
5.248LeuSer: 5.248 ± 0.584
4.898LeuThr: 4.898 ± 0.562
4.618LeuVal: 4.618 ± 0.687
0.91LeuTrp: 0.91 ± 0.235
1.749LeuTyr: 1.749 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
2.729MetAla: 2.729 ± 0.488
0.28MetCys: 0.28 ± 0.145
1.609MetAsp: 1.609 ± 0.401
2.589MetGlu: 2.589 ± 0.465
1.399MetPhe: 1.399 ± 0.33
1.19MetGly: 1.19 ± 0.312
0.63MetHis: 0.63 ± 0.225
2.519MetIle: 2.519 ± 0.492
2.659MetLys: 2.659 ± 0.475
2.239MetLeu: 2.239 ± 0.405
1.539MetMet: 1.539 ± 0.329
1.609MetAsn: 1.609 ± 0.301
0.84MetPro: 0.84 ± 0.209
1.05MetGln: 1.05 ± 0.301
1.749MetArg: 1.749 ± 0.362
2.309MetSer: 2.309 ± 0.388
1.469MetThr: 1.469 ± 0.374
1.959MetVal: 1.959 ± 0.345
0.42MetTrp: 0.42 ± 0.164
0.63MetTyr: 0.63 ± 0.24
0.0MetXaa: 0.0 ± 0.0
Asn
4.198AsnAla: 4.198 ± 0.775
0.28AsnCys: 0.28 ± 0.129
2.939AsnAsp: 2.939 ± 0.438
2.939AsnGlu: 2.939 ± 0.416
2.449AsnPhe: 2.449 ± 0.377
4.198AsnGly: 4.198 ± 0.588
0.98AsnHis: 0.98 ± 0.278
2.729AsnIle: 2.729 ± 0.453
2.729AsnLys: 2.729 ± 0.497
2.869AsnLeu: 2.869 ± 0.497
1.19AsnMet: 1.19 ± 0.288
2.239AsnAsn: 2.239 ± 0.404
2.169AsnPro: 2.169 ± 0.42
2.099AsnGln: 2.099 ± 0.356
1.819AsnArg: 1.819 ± 0.389
3.219AsnSer: 3.219 ± 0.506
1.819AsnThr: 1.819 ± 0.307
3.849AsnVal: 3.849 ± 0.593
0.7AsnTrp: 0.7 ± 0.176
1.469AsnTyr: 1.469 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
2.099ProAla: 2.099 ± 0.334
0.35ProCys: 0.35 ± 0.16
2.029ProAsp: 2.029 ± 0.38
2.939ProGlu: 2.939 ± 0.491
1.12ProPhe: 1.12 ± 0.308
2.659ProGly: 2.659 ± 0.424
0.63ProHis: 0.63 ± 0.191
2.029ProIle: 2.029 ± 0.358
1.539ProLys: 1.539 ± 0.449
1.679ProLeu: 1.679 ± 0.341
0.98ProMet: 0.98 ± 0.271
1.889ProAsn: 1.889 ± 0.355
1.19ProPro: 1.19 ± 0.338
1.19ProGln: 1.19 ± 0.312
1.19ProArg: 1.19 ± 0.286
1.889ProSer: 1.889 ± 0.405
1.889ProThr: 1.889 ± 0.329
3.079ProVal: 3.079 ± 0.363
0.35ProTrp: 0.35 ± 0.169
1.469ProTyr: 1.469 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
3.429GlnAla: 3.429 ± 0.491
0.28GlnCys: 0.28 ± 0.12
1.889GlnAsp: 1.889 ± 0.307
1.959GlnGlu: 1.959 ± 0.437
1.12GlnPhe: 1.12 ± 0.252
2.169GlnGly: 2.169 ± 0.451
0.63GlnHis: 0.63 ± 0.202
2.799GlnIle: 2.799 ± 0.454
1.889GlnLys: 1.889 ± 0.355
3.639GlnLeu: 3.639 ± 0.498
1.12GlnMet: 1.12 ± 0.224
1.889GlnAsn: 1.889 ± 0.376
1.19GlnPro: 1.19 ± 0.303
2.309GlnGln: 2.309 ± 0.563
1.889GlnArg: 1.889 ± 0.434
2.659GlnSer: 2.659 ± 0.471
1.819GlnThr: 1.819 ± 0.386
2.799GlnVal: 2.799 ± 0.326
0.77GlnTrp: 0.77 ± 0.252
1.679GlnTyr: 1.679 ± 0.355
0.0GlnXaa: 0.0 ± 0.0
Arg
3.779ArgAla: 3.779 ± 0.602
0.91ArgCys: 0.91 ± 0.391
2.939ArgAsp: 2.939 ± 0.638
3.639ArgGlu: 3.639 ± 0.475
2.379ArgPhe: 2.379 ± 0.426
3.219ArgGly: 3.219 ± 0.379
0.91ArgHis: 0.91 ± 0.237
2.869ArgIle: 2.869 ± 0.495
3.639ArgLys: 3.639 ± 0.468
3.499ArgLeu: 3.499 ± 0.542
1.399ArgMet: 1.399 ± 0.346
1.959ArgAsn: 1.959 ± 0.357
1.819ArgPro: 1.819 ± 0.398
1.469ArgGln: 1.469 ± 0.347
3.149ArgArg: 3.149 ± 0.587
2.729ArgSer: 2.729 ± 0.368
1.609ArgThr: 1.609 ± 0.323
4.128ArgVal: 4.128 ± 0.59
0.91ArgTrp: 0.91 ± 0.243
2.099ArgTyr: 2.099 ± 0.42
0.0ArgXaa: 0.0 ± 0.0
Ser
5.248SerAla: 5.248 ± 0.93
0.84SerCys: 0.84 ± 0.26
4.408SerAsp: 4.408 ± 0.589
3.849SerGlu: 3.849 ± 0.485
2.659SerPhe: 2.659 ± 0.37
6.857SerGly: 6.857 ± 0.9
1.05SerHis: 1.05 ± 0.294
4.058SerIle: 4.058 ± 0.533
4.058SerLys: 4.058 ± 0.672
5.388SerLeu: 5.388 ± 0.613
1.609SerMet: 1.609 ± 0.303
2.099SerAsn: 2.099 ± 0.369
1.819SerPro: 1.819 ± 0.388
2.729SerGln: 2.729 ± 0.373
3.359SerArg: 3.359 ± 0.439
4.338SerSer: 4.338 ± 0.581
3.359SerThr: 3.359 ± 0.383
4.828SerVal: 4.828 ± 0.519
0.98SerTrp: 0.98 ± 0.303
2.099SerTyr: 2.099 ± 0.372
0.0SerXaa: 0.0 ± 0.0
Thr
4.338ThrAla: 4.338 ± 0.596
0.84ThrCys: 0.84 ± 0.247
3.639ThrAsp: 3.639 ± 0.581
3.499ThrGlu: 3.499 ± 0.549
2.939ThrPhe: 2.939 ± 0.462
4.618ThrGly: 4.618 ± 0.459
0.91ThrHis: 0.91 ± 0.268
3.849ThrIle: 3.849 ± 0.481
3.569ThrLys: 3.569 ± 0.543
3.219ThrLeu: 3.219 ± 0.435
1.749ThrMet: 1.749 ± 0.384
2.309ThrAsn: 2.309 ± 0.435
3.079ThrPro: 3.079 ± 0.547
2.169ThrGln: 2.169 ± 0.365
2.239ThrArg: 2.239 ± 0.379
3.849ThrSer: 3.849 ± 0.42
3.289ThrThr: 3.289 ± 0.61
3.849ThrVal: 3.849 ± 0.549
0.7ThrTrp: 0.7 ± 0.198
2.099ThrTyr: 2.099 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
4.898ValAla: 4.898 ± 0.581
0.84ValCys: 0.84 ± 0.255
4.688ValAsp: 4.688 ± 0.64
4.478ValGlu: 4.478 ± 0.576
2.799ValPhe: 2.799 ± 0.45
3.079ValGly: 3.079 ± 0.51
1.539ValHis: 1.539 ± 0.374
4.268ValIle: 4.268 ± 0.568
6.368ValLys: 6.368 ± 0.67
3.289ValLeu: 3.289 ± 0.526
1.609ValMet: 1.609 ± 0.335
3.709ValAsn: 3.709 ± 0.496
2.519ValPro: 2.519 ± 0.433
3.219ValGln: 3.219 ± 0.417
4.338ValArg: 4.338 ± 0.519
4.898ValSer: 4.898 ± 0.471
4.688ValThr: 4.688 ± 0.705
4.758ValVal: 4.758 ± 0.606
1.05ValTrp: 1.05 ± 0.209
1.819ValTyr: 1.819 ± 0.347
0.0ValXaa: 0.0 ± 0.0
Trp
1.19TrpAla: 1.19 ± 0.261
0.28TrpCys: 0.28 ± 0.167
0.91TrpAsp: 0.91 ± 0.246
0.77TrpGlu: 0.77 ± 0.205
0.35TrpPhe: 0.35 ± 0.176
0.98TrpGly: 0.98 ± 0.215
0.42TrpHis: 0.42 ± 0.156
0.84TrpIle: 0.84 ± 0.205
0.91TrpLys: 0.91 ± 0.26
1.399TrpLeu: 1.399 ± 0.309
0.56TrpMet: 0.56 ± 0.176
0.77TrpAsn: 0.77 ± 0.24
0.35TrpPro: 0.35 ± 0.152
0.56TrpGln: 0.56 ± 0.168
1.05TrpArg: 1.05 ± 0.267
0.84TrpSer: 0.84 ± 0.299
1.12TrpThr: 1.12 ± 0.316
0.7TrpVal: 0.7 ± 0.212
0.21TrpTrp: 0.21 ± 0.102
0.49TrpTyr: 0.49 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.009TyrAla: 3.009 ± 0.436
0.35TyrCys: 0.35 ± 0.162
2.869TyrAsp: 2.869 ± 0.374
1.959TyrGlu: 1.959 ± 0.382
1.19TyrPhe: 1.19 ± 0.331
2.589TyrGly: 2.589 ± 0.422
0.63TyrHis: 0.63 ± 0.209
2.659TyrIle: 2.659 ± 0.472
2.239TyrLys: 2.239 ± 0.352
1.679TyrLeu: 1.679 ± 0.412
1.26TyrMet: 1.26 ± 0.26
1.679TyrAsn: 1.679 ± 0.347
1.19TyrPro: 1.19 ± 0.312
1.679TyrGln: 1.679 ± 0.334
2.169TyrArg: 2.169 ± 0.313
2.239TyrSer: 2.239 ± 0.345
2.099TyrThr: 2.099 ± 0.473
1.679TyrVal: 1.679 ± 0.34
0.35TyrTrp: 0.35 ± 0.177
1.539TyrTyr: 1.539 ± 0.272
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (14292 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski