Amino acid dipepetide frequency for Micromonospora siamensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.375AlaAla: 23.375 ± 0.215
0.94AlaCys: 0.94 ± 0.025
9.071AlaAsp: 9.071 ± 0.083
8.447AlaGlu: 8.447 ± 0.089
3.222AlaPhe: 3.222 ± 0.046
15.696AlaGly: 15.696 ± 0.12
2.535AlaHis: 2.535 ± 0.044
3.534AlaIle: 3.534 ± 0.054
2.323AlaLys: 2.323 ± 0.049
14.874AlaLeu: 14.874 ± 0.138
2.316AlaMet: 2.316 ± 0.035
2.124AlaAsn: 2.124 ± 0.041
7.453AlaPro: 7.453 ± 0.094
3.546AlaGln: 3.546 ± 0.047
10.645AlaArg: 10.645 ± 0.084
5.15AlaSer: 5.15 ± 0.049
8.249AlaThr: 8.249 ± 0.091
13.317AlaVal: 13.317 ± 0.119
1.904AlaTrp: 1.904 ± 0.032
2.957AlaTyr: 2.957 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.972CysAla: 0.972 ± 0.024
0.093CysCys: 0.093 ± 0.008
0.472CysAsp: 0.472 ± 0.015
0.286CysGlu: 0.286 ± 0.013
0.221CysPhe: 0.221 ± 0.012
0.883CysGly: 0.883 ± 0.023
0.182CysHis: 0.182 ± 0.01
0.127CysIle: 0.127 ± 0.009
0.087CysLys: 0.087 ± 0.007
0.703CysLeu: 0.703 ± 0.02
0.101CysMet: 0.101 ± 0.007
0.128CysAsn: 0.128 ± 0.009
0.482CysPro: 0.482 ± 0.017
0.175CysGln: 0.175 ± 0.009
0.626CysArg: 0.626 ± 0.022
0.342CysSer: 0.342 ± 0.014
0.428CysThr: 0.428 ± 0.016
0.601CysVal: 0.601 ± 0.018
0.148CysTrp: 0.148 ± 0.01
0.153CysTyr: 0.153 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.764AspAla: 7.764 ± 0.071
0.407AspCys: 0.407 ± 0.014
3.769AspAsp: 3.769 ± 0.059
3.754AspGlu: 3.754 ± 0.057
1.485AspPhe: 1.485 ± 0.032
6.566AspGly: 6.566 ± 0.066
1.235AspHis: 1.235 ± 0.028
1.478AspIle: 1.478 ± 0.027
0.924AspLys: 0.924 ± 0.03
6.714AspLeu: 6.714 ± 0.072
0.69AspMet: 0.69 ± 0.016
0.915AspAsn: 0.915 ± 0.027
5.19AspPro: 5.19 ± 0.057
1.716AspGln: 1.716 ± 0.031
6.0AspArg: 6.0 ± 0.066
2.123AspSer: 2.123 ± 0.035
2.863AspThr: 2.863 ± 0.048
5.081AspVal: 5.081 ± 0.059
1.034AspTrp: 1.034 ± 0.026
1.089AspTyr: 1.089 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
6.408GluAla: 6.408 ± 0.065
0.327GluCys: 0.327 ± 0.014
2.039GluAsp: 2.039 ± 0.04
2.557GluGlu: 2.557 ± 0.051
1.411GluPhe: 1.411 ± 0.028
3.247GluGly: 3.247 ± 0.06
1.323GluHis: 1.323 ± 0.035
2.067GluIle: 2.067 ± 0.033
1.003GluLys: 1.003 ± 0.026
6.73GluLeu: 6.73 ± 0.083
0.793GluMet: 0.793 ± 0.02
0.817GluAsn: 0.817 ± 0.024
3.504GluPro: 3.504 ± 0.057
2.176GluGln: 2.176 ± 0.042
5.221GluArg: 5.221 ± 0.067
2.082GluSer: 2.082 ± 0.031
2.419GluThr: 2.419 ± 0.034
4.654GluVal: 4.654 ± 0.06
0.677GluTrp: 0.677 ± 0.021
1.016GluTyr: 1.016 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.817PheAla: 3.817 ± 0.044
0.247PheCys: 0.247 ± 0.011
1.999PheAsp: 1.999 ± 0.041
1.12PheGlu: 1.12 ± 0.026
0.869PhePhe: 0.869 ± 0.021
2.994PheGly: 2.994 ± 0.043
0.57PheHis: 0.57 ± 0.02
0.617PheIle: 0.617 ± 0.02
0.381PheLys: 0.381 ± 0.016
2.534PheLeu: 2.534 ± 0.044
0.307PheMet: 0.307 ± 0.014
0.543PheAsn: 0.543 ± 0.022
1.329PhePro: 1.329 ± 0.027
0.58PheGln: 0.58 ± 0.017
1.815PheArg: 1.815 ± 0.037
1.269PheSer: 1.269 ± 0.029
1.93PheThr: 1.93 ± 0.034
2.41PheVal: 2.41 ± 0.039
0.418PheTrp: 0.418 ± 0.017
0.577PheTyr: 0.577 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.467GlyAla: 11.467 ± 0.105
0.81GlyCys: 0.81 ± 0.023
5.53GlyAsp: 5.53 ± 0.073
4.8GlyGlu: 4.8 ± 0.05
2.824GlyPhe: 2.824 ± 0.048
9.232GlyGly: 9.232 ± 0.096
2.14GlyHis: 2.14 ± 0.037
2.993GlyIle: 2.993 ± 0.047
1.863GlyLys: 1.863 ± 0.041
9.869GlyLeu: 9.869 ± 0.09
1.886GlyMet: 1.886 ± 0.034
1.77GlyAsn: 1.77 ± 0.039
5.722GlyPro: 5.722 ± 0.065
2.833GlyGln: 2.833 ± 0.054
8.713GlyArg: 8.713 ± 0.074
4.755GlySer: 4.755 ± 0.057
6.033GlyThr: 6.033 ± 0.08
8.378GlyVal: 8.378 ± 0.08
2.13GlyTrp: 2.13 ± 0.037
2.433GlyTyr: 2.433 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.335HisAla: 2.335 ± 0.04
0.175HisCys: 0.175 ± 0.011
1.288HisAsp: 1.288 ± 0.031
1.032HisGlu: 1.032 ± 0.024
0.556HisPhe: 0.556 ± 0.016
2.143HisGly: 2.143 ± 0.038
0.615HisHis: 0.615 ± 0.021
0.467HisIle: 0.467 ± 0.016
0.248HisLys: 0.248 ± 0.012
2.381HisLeu: 2.381 ± 0.04
0.265HisMet: 0.265 ± 0.012
0.318HisAsn: 0.318 ± 0.014
1.766HisPro: 1.766 ± 0.029
0.571HisGln: 0.571 ± 0.02
2.147HisArg: 2.147 ± 0.041
0.822HisSer: 0.822 ± 0.023
1.042HisThr: 1.042 ± 0.025
1.656HisVal: 1.656 ± 0.03
0.354HisTrp: 0.354 ± 0.016
0.414HisTyr: 0.414 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.369IleAla: 4.369 ± 0.055
0.269IleCys: 0.269 ± 0.013
2.1IleAsp: 2.1 ± 0.037
1.781IleGlu: 1.781 ± 0.038
0.833IlePhe: 0.833 ± 0.023
3.288IleGly: 3.288 ± 0.048
0.488IleHis: 0.488 ± 0.018
0.791IleIle: 0.791 ± 0.022
0.576IleLys: 0.576 ± 0.024
2.242IleLeu: 2.242 ± 0.037
0.384IleMet: 0.384 ± 0.013
0.65IleAsn: 0.65 ± 0.02
1.563IlePro: 1.563 ± 0.03
0.65IleGln: 0.65 ± 0.02
2.259IleArg: 2.259 ± 0.036
1.554IleSer: 1.554 ± 0.03
1.939IleThr: 1.939 ± 0.038
2.676IleVal: 2.676 ± 0.042
0.392IleTrp: 0.392 ± 0.014
0.535IleTyr: 0.535 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
2.127LysAla: 2.127 ± 0.052
0.097LysCys: 0.097 ± 0.008
0.817LysAsp: 0.817 ± 0.026
0.795LysGlu: 0.795 ± 0.026
0.391LysPhe: 0.391 ± 0.016
1.309LysGly: 1.309 ± 0.036
0.307LysHis: 0.307 ± 0.013
0.735LysIle: 0.735 ± 0.025
0.507LysLys: 0.507 ± 0.025
1.783LysLeu: 1.783 ± 0.038
0.3LysMet: 0.3 ± 0.014
0.352LysAsn: 0.352 ± 0.017
1.085LysPro: 1.085 ± 0.03
0.567LysGln: 0.567 ± 0.022
1.177LysArg: 1.177 ± 0.028
0.832LysSer: 0.832 ± 0.024
0.943LysThr: 0.943 ± 0.027
1.629LysVal: 1.629 ± 0.037
0.217LysTrp: 0.217 ± 0.013
0.349LysTyr: 0.349 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
17.188LeuAla: 17.188 ± 0.131
0.724LeuCys: 0.724 ± 0.02
6.965LeuAsp: 6.965 ± 0.071
3.776LeuGlu: 3.776 ± 0.054
2.647LeuPhe: 2.647 ± 0.045
9.663LeuGly: 9.663 ± 0.091
2.25LeuHis: 2.25 ± 0.036
3.136LeuIle: 3.136 ± 0.046
1.379LeuLys: 1.379 ± 0.03
12.237LeuLeu: 12.237 ± 0.144
1.303LeuMet: 1.303 ± 0.026
1.589LeuAsn: 1.589 ± 0.032
6.984LeuPro: 6.984 ± 0.073
1.775LeuGln: 1.775 ± 0.033
9.503LeuArg: 9.503 ± 0.087
4.674LeuSer: 4.674 ± 0.057
7.19LeuThr: 7.19 ± 0.071
10.355LeuVal: 10.355 ± 0.09
1.291LeuTrp: 1.291 ± 0.035
1.676LeuTyr: 1.676 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.004MetAla: 2.004 ± 0.036
0.102MetCys: 0.102 ± 0.007
0.698MetAsp: 0.698 ± 0.018
0.561MetGlu: 0.561 ± 0.017
0.437MetPhe: 0.437 ± 0.015
1.106MetGly: 1.106 ± 0.027
0.269MetHis: 0.269 ± 0.012
0.636MetIle: 0.636 ± 0.022
0.311MetLys: 0.311 ± 0.012
1.627MetLeu: 1.627 ± 0.033
0.246MetMet: 0.246 ± 0.011
0.35MetAsn: 0.35 ± 0.013
1.009MetPro: 1.009 ± 0.022
0.393MetGln: 0.393 ± 0.015
1.338MetArg: 1.338 ± 0.027
1.174MetSer: 1.174 ± 0.027
1.505MetThr: 1.505 ± 0.03
1.288MetVal: 1.288 ± 0.026
0.186MetTrp: 0.186 ± 0.011
0.269MetTyr: 0.269 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.079AsnAla: 2.079 ± 0.043
0.155AsnCys: 0.155 ± 0.01
0.94AsnAsp: 0.94 ± 0.024
0.695AsnGlu: 0.695 ± 0.021
0.486AsnPhe: 0.486 ± 0.016
1.778AsnGly: 1.778 ± 0.043
0.377AsnHis: 0.377 ± 0.013
0.519AsnIle: 0.519 ± 0.017
0.311AsnLys: 0.311 ± 0.014
1.794AsnLeu: 1.794 ± 0.033
0.267AsnMet: 0.267 ± 0.013
0.444AsnAsn: 0.444 ± 0.019
1.396AsnPro: 1.396 ± 0.029
0.557AsnGln: 0.557 ± 0.019
1.375AsnArg: 1.375 ± 0.03
0.829AsnSer: 0.829 ± 0.025
1.036AsnThr: 1.036 ± 0.034
1.393AsnVal: 1.393 ± 0.03
0.314AsnTrp: 0.314 ± 0.014
0.394AsnTyr: 0.394 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
10.606ProAla: 10.606 ± 0.104
0.3ProCys: 0.3 ± 0.013
4.715ProAsp: 4.715 ± 0.059
4.03ProGlu: 4.03 ± 0.059
1.427ProPhe: 1.427 ± 0.025
6.908ProGly: 6.908 ± 0.079
1.177ProHis: 1.177 ± 0.028
1.501ProIle: 1.501 ± 0.03
0.995ProLys: 0.995 ± 0.031
5.462ProLeu: 5.462 ± 0.063
0.979ProMet: 0.979 ± 0.026
0.981ProAsn: 0.981 ± 0.025
4.377ProPro: 4.377 ± 0.085
1.623ProGln: 1.623 ± 0.033
4.155ProArg: 4.155 ± 0.057
2.875ProSer: 2.875 ± 0.042
4.203ProThr: 4.203 ± 0.066
6.404ProVal: 6.404 ± 0.061
0.98ProTrp: 0.98 ± 0.025
1.308ProTyr: 1.308 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.501GlnAla: 3.501 ± 0.052
0.139GlnCys: 0.139 ± 0.009
1.093GlnAsp: 1.093 ± 0.027
1.156GlnGlu: 1.156 ± 0.026
0.681GlnPhe: 0.681 ± 0.018
1.909GlnGly: 1.909 ± 0.035
0.541GlnHis: 0.541 ± 0.021
1.006GlnIle: 1.006 ± 0.021
0.433GlnLys: 0.433 ± 0.017
3.229GlnLeu: 3.229 ± 0.051
0.392GlnMet: 0.392 ± 0.014
0.476GlnAsn: 0.476 ± 0.02
1.864GlnPro: 1.864 ± 0.039
1.142GlnGln: 1.142 ± 0.037
2.76GlnArg: 2.76 ± 0.041
1.06GlnSer: 1.06 ± 0.023
1.24GlnThr: 1.24 ± 0.028
2.851GlnVal: 2.851 ± 0.044
0.477GlnTrp: 0.477 ± 0.016
0.51GlnTyr: 0.51 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
10.691ArgAla: 10.691 ± 0.107
0.607ArgCys: 0.607 ± 0.02
4.781ArgAsp: 4.781 ± 0.057
4.437ArgGlu: 4.437 ± 0.066
2.459ArgPhe: 2.459 ± 0.038
6.015ArgGly: 6.015 ± 0.061
2.172ArgHis: 2.172 ± 0.038
3.073ArgIle: 3.073 ± 0.039
1.267ArgLys: 1.267 ± 0.031
9.655ArgLeu: 9.655 ± 0.105
1.88ArgMet: 1.88 ± 0.035
1.389ArgAsn: 1.389 ± 0.029
5.941ArgPro: 5.941 ± 0.068
2.602ArgGln: 2.602 ± 0.039
9.347ArgArg: 9.347 ± 0.112
4.155ArgSer: 4.155 ± 0.053
5.012ArgThr: 5.012 ± 0.062
6.722ArgVal: 6.722 ± 0.061
1.765ArgTrp: 1.765 ± 0.034
2.009ArgTyr: 2.009 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.121SerAla: 6.121 ± 0.076
0.387SerCys: 0.387 ± 0.017
2.257SerAsp: 2.257 ± 0.033
1.788SerGlu: 1.788 ± 0.035
1.367SerPhe: 1.367 ± 0.03
5.269SerGly: 5.269 ± 0.068
0.823SerHis: 0.823 ± 0.024
1.357SerIle: 1.357 ± 0.03
0.724SerLys: 0.724 ± 0.022
4.09SerLeu: 4.09 ± 0.049
0.919SerMet: 0.919 ± 0.023
0.8SerAsn: 0.8 ± 0.024
3.031SerPro: 3.031 ± 0.04
1.03SerGln: 1.03 ± 0.026
3.598SerArg: 3.598 ± 0.049
2.264SerSer: 2.264 ± 0.042
2.995SerThr: 2.995 ± 0.046
3.943SerVal: 3.943 ± 0.043
0.933SerTrp: 0.933 ± 0.023
1.19SerTyr: 1.19 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
8.786ThrAla: 8.786 ± 0.093
0.417ThrCys: 0.417 ± 0.016
3.809ThrAsp: 3.809 ± 0.047
2.95ThrGlu: 2.95 ± 0.041
1.585ThrPhe: 1.585 ± 0.029
7.068ThrGly: 7.068 ± 0.067
1.01ThrHis: 1.01 ± 0.027
1.77ThrIle: 1.77 ± 0.032
0.938ThrLys: 0.938 ± 0.029
5.836ThrLeu: 5.836 ± 0.069
0.921ThrMet: 0.921 ± 0.023
1.058ThrAsn: 1.058 ± 0.033
4.328ThrPro: 4.328 ± 0.059
1.176ThrGln: 1.176 ± 0.026
4.116ThrArg: 4.116 ± 0.049
2.9ThrSer: 2.9 ± 0.049
3.851ThrThr: 3.851 ± 0.071
6.599ThrVal: 6.599 ± 0.07
0.907ThrTrp: 0.907 ± 0.024
1.288ThrTyr: 1.288 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
13.372ValAla: 13.372 ± 0.113
0.676ValCys: 0.676 ± 0.02
6.166ValAsp: 6.166 ± 0.064
4.905ValGlu: 4.905 ± 0.067
2.309ValPhe: 2.309 ± 0.037
7.937ValGly: 7.937 ± 0.076
1.782ValHis: 1.782 ± 0.035
2.634ValIle: 2.634 ± 0.042
1.463ValLys: 1.463 ± 0.036
10.252ValLeu: 10.252 ± 0.09
1.11ValMet: 1.11 ± 0.022
1.66ValAsn: 1.66 ± 0.035
5.832ValPro: 5.832 ± 0.064
2.053ValGln: 2.053 ± 0.036
7.592ValArg: 7.592 ± 0.078
4.129ValSer: 4.129 ± 0.05
6.31ValThr: 6.31 ± 0.084
9.245ValVal: 9.245 ± 0.1
1.168ValTrp: 1.168 ± 0.025
1.431ValTyr: 1.431 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.761TrpAla: 1.761 ± 0.036
0.168TrpCys: 0.168 ± 0.01
0.767TrpAsp: 0.767 ± 0.023
0.617TrpGlu: 0.617 ± 0.02
0.539TrpPhe: 0.539 ± 0.017
1.021TrpGly: 1.021 ± 0.023
0.367TrpHis: 0.367 ± 0.013
0.528TrpIle: 0.528 ± 0.017
0.286TrpLys: 0.286 ± 0.014
2.051TrpLeu: 2.051 ± 0.034
0.26TrpMet: 0.26 ± 0.013
0.401TrpAsn: 0.401 ± 0.017
0.942TrpPro: 0.942 ± 0.024
0.659TrpGln: 0.659 ± 0.019
1.676TrpArg: 1.676 ± 0.035
1.037TrpSer: 1.037 ± 0.027
1.047TrpThr: 1.047 ± 0.024
1.125TrpVal: 1.125 ± 0.026
0.422TrpTrp: 0.422 ± 0.018
0.39TrpTyr: 0.39 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.935TyrAla: 2.935 ± 0.039
0.169TyrCys: 0.169 ± 0.011
1.518TyrAsp: 1.518 ± 0.039
0.985TyrGlu: 0.985 ± 0.024
0.579TyrPhe: 0.579 ± 0.019
2.148TyrGly: 2.148 ± 0.047
0.432TyrHis: 0.432 ± 0.016
0.368TyrIle: 0.368 ± 0.016
0.301TyrLys: 0.301 ± 0.014
2.242TyrLeu: 2.242 ± 0.036
0.188TyrMet: 0.188 ± 0.011
0.376TyrAsn: 0.376 ± 0.02
1.208TyrPro: 1.208 ± 0.032
0.634TyrGln: 0.634 ± 0.019
1.915TyrArg: 1.915 ± 0.036
0.862TyrSer: 0.862 ± 0.024
1.078TyrThr: 1.078 ± 0.026
1.7TyrVal: 1.7 ± 0.03
0.348TyrTrp: 0.348 ± 0.014
0.421TyrTyr: 0.421 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5581 proteins (1848279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski