Chili leaf curl betasatellite
Average proteome isoelectric point is 4.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T476|Q7T476_9VIRU C1 protein OS=Chili leaf curl betasatellite OX=2010286 GN=C1 PE=4 SV=1
MM1 pKa = 6.76 HH2 pKa = 7.23 HH3 pKa = 7.02 VYY5 pKa = 9.97 EE6 pKa = 4.63 LCPYY10 pKa = 8.2 IQIYY14 pKa = 8.7 GHH16 pKa = 5.39 YY17 pKa = 10.14 LRR19 pKa = 11.84 RR20 pKa = 11.84 QDD22 pKa = 3.06 SCIVHH27 pKa = 7.65 IYY29 pKa = 8.71 MQMTIKK35 pKa = 10.68 YY36 pKa = 8.48 NNEE39 pKa = 3.25 KK40 pKa = 10.51 GMEE43 pKa = 3.71 FRR45 pKa = 11.84 IDD47 pKa = 3.45 VKK49 pKa = 10.89 INEE52 pKa = 4.55 DD53 pKa = 3.28 NSILVLVEE61 pKa = 4.17 LCSTRR66 pKa = 11.84 SPALAKK72 pKa = 9.01 KK73 pKa = 8.95 TFMIPYY79 pKa = 8.61 GHH81 pKa = 7.44 DD82 pKa = 3.85 GIIPPFDD89 pKa = 4.09 FNILEE94 pKa = 4.18 EE95 pKa = 4.99 GIHH98 pKa = 6.55 NILKK102 pKa = 10.38 VMYY105 pKa = 8.74 KK106 pKa = 10.17 DD107 pKa = 3.43 SRR109 pKa = 11.84 IGEE112 pKa = 4.06 FRR114 pKa = 11.84 QEE116 pKa = 4.36 DD117 pKa = 3.99 MVEE120 pKa = 4.21 TIDD123 pKa = 3.54 MLMMHH128 pKa = 7.23 EE129 pKa = 4.74 ASVIDD134 pKa = 3.78 IRR136 pKa = 11.84 IADD139 pKa = 4.26 EE140 pKa = 4.45 YY141 pKa = 11.36 DD142 pKa = 3.12 VCTNVCVV149 pKa = 3.48
Molecular weight: 17.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.871
IPC2_protein 5.054
IPC_protein 4.991
Toseland 5.003
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.143
Wikipedia 4.914
Rodwell 4.952
Grimsley 4.952
Solomon 5.029
Lehninger 4.991
Nozaki 5.169
DTASelect 5.321
Thurlkill 5.029
EMBOSS 4.991
Sillero 5.245
Patrickios 2.854
IPC_peptide 5.029
IPC2_peptide 5.245
IPC2.peptide.svr19 5.127
Protein with the highest isoelectric point:
>tr|Q7T476|Q7T476_9VIRU C1 protein OS=Chili leaf curl betasatellite OX=2010286 GN=C1 PE=4 SV=1
MM1 pKa = 6.76 HH2 pKa = 7.23 HH3 pKa = 7.02 VYY5 pKa = 9.97 EE6 pKa = 4.63 LCPYY10 pKa = 8.2 IQIYY14 pKa = 8.7 GHH16 pKa = 5.39 YY17 pKa = 10.14 LRR19 pKa = 11.84 RR20 pKa = 11.84 QDD22 pKa = 3.06 SCIVHH27 pKa = 7.65 IYY29 pKa = 8.71 MQMTIKK35 pKa = 10.68 YY36 pKa = 8.48 NNEE39 pKa = 3.25 KK40 pKa = 10.51 GMEE43 pKa = 3.71 FRR45 pKa = 11.84 IDD47 pKa = 3.45 VKK49 pKa = 10.89 INEE52 pKa = 4.55 DD53 pKa = 3.28 NSILVLVEE61 pKa = 4.17 LCSTRR66 pKa = 11.84 SPALAKK72 pKa = 9.01 KK73 pKa = 8.95 TFMIPYY79 pKa = 8.61 GHH81 pKa = 7.44 DD82 pKa = 3.85 GIIPPFDD89 pKa = 4.09 FNILEE94 pKa = 4.18 EE95 pKa = 4.99 GIHH98 pKa = 6.55 NILKK102 pKa = 10.38 VMYY105 pKa = 8.74 KK106 pKa = 10.17 DD107 pKa = 3.43 SRR109 pKa = 11.84 IGEE112 pKa = 4.06 FRR114 pKa = 11.84 QEE116 pKa = 4.36 DD117 pKa = 3.99 MVEE120 pKa = 4.21 TIDD123 pKa = 3.54 MLMMHH128 pKa = 7.23 EE129 pKa = 4.74 ASVIDD134 pKa = 3.78 IRR136 pKa = 11.84 IADD139 pKa = 4.26 EE140 pKa = 4.45 YY141 pKa = 11.36 DD142 pKa = 3.12 VCTNVCVV149 pKa = 3.48
Molecular weight: 17.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.871
IPC2_protein 5.054
IPC_protein 4.991
Toseland 5.003
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.143
Wikipedia 4.914
Rodwell 4.952
Grimsley 4.952
Solomon 5.029
Lehninger 4.991
Nozaki 5.169
DTASelect 5.321
Thurlkill 5.029
EMBOSS 4.991
Sillero 5.245
Patrickios 2.854
IPC_peptide 5.029
IPC2_peptide 5.245
IPC2.peptide.svr19 5.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
149
149
149
149.0
17.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.685 ± 0.0
3.356 ± 0.0
7.383 ± 0.0
8.054 ± 0.0
3.356 ± 0.0
4.027 ± 0.0
4.698 ± 0.0
12.752 ± 0.0
4.698 ± 0.0
6.04 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.711 ± 0.0
4.698 ± 0.0
3.356 ± 0.0
2.685 ± 0.0
4.698 ± 0.0
4.027 ± 0.0
3.356 ± 0.0
7.383 ± 0.0
0.0 ± 0.0
6.04 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here