Sida golden yellow spot virus
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S6KEF0|A0A1S6KEF0_9GEMI AC4 protein OS=Sida golden yellow spot virus OX=1949198 PE=3 SV=1
MM1 pKa = 7.8 DD2 pKa = 4.31 QLFCQLEE9 pKa = 4.34 GNKK12 pKa = 9.43 IEE14 pKa = 4.95 PKK16 pKa = 10.37 DD17 pKa = 3.66 LLPAVSRR24 pKa = 11.84 WIVEE28 pKa = 4.01 KK29 pKa = 10.28 GTAQQFIDD37 pKa = 4.94 WINGLQAEE45 pKa = 4.73 TKK47 pKa = 10.68 GLFHH51 pKa = 7.5 ALNAVPDD58 pKa = 4.15 GEE60 pKa = 4.88 EE61 pKa = 3.79 IQTDD65 pKa = 3.58 SAEE68 pKa = 4.1 VDD70 pKa = 3.56 GEE72 pKa = 4.38 EE73 pKa = 4.13 EE74 pKa = 4.22 TEE76 pKa = 4.17 LRR78 pKa = 11.84 LSQPVRR84 pKa = 11.84 ISGTGKK90 pKa = 9.08 EE91 pKa = 4.04 QGSYY95 pKa = 9.93 RR96 pKa = 11.84 QIPIDD101 pKa = 3.29
Molecular weight: 11.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.534
IPC2_protein 4.38
IPC_protein 4.266
Toseland 4.113
ProMoST 4.291
Dawson 4.215
Bjellqvist 4.406
Wikipedia 4.075
Rodwell 4.101
Grimsley 4.024
Solomon 4.202
Lehninger 4.151
Nozaki 4.329
DTASelect 4.444
Thurlkill 4.113
EMBOSS 4.101
Sillero 4.368
Patrickios 4.024
IPC_peptide 4.215
IPC2_peptide 4.368
IPC2.peptide.svr19 4.292
Protein with the highest isoelectric point:
>tr|A0A1S6KEF2|A0A1S6KEF2_9GEMI Replication-associated protein OS=Sida golden yellow spot virus OX=1949198 PE=3 SV=1
MM1 pKa = 7.6 GNLISTCLSNSKK13 pKa = 10.43 ASSKK17 pKa = 10.4 PRR19 pKa = 11.84 IADD22 pKa = 3.24 SSTWFPQPGQYY33 pKa = 10.22 ISIQTFRR40 pKa = 11.84 EE41 pKa = 3.97 LNQAQTSRR49 pKa = 11.84 PTSRR53 pKa = 11.84 RR54 pKa = 11.84 TEE56 pKa = 4.05 TPSNGEE62 pKa = 3.88 NSRR65 pKa = 11.84 STQEE69 pKa = 3.71 VLEE72 pKa = 4.27 EE73 pKa = 3.85 VSRR76 pKa = 11.84 RR77 pKa = 11.84 LTSLTPRR84 pKa = 11.84 SS85 pKa = 3.59
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.297
IPC_protein 10.058
Toseland 10.394
ProMoST 10.101
Dawson 10.511
Bjellqvist 10.233
Wikipedia 10.716
Rodwell 10.599
Grimsley 10.555
Solomon 10.657
Lehninger 10.628
Nozaki 10.409
DTASelect 10.218
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.452
Patrickios 10.496
IPC_peptide 10.657
IPC2_peptide 9.385
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1075
85
359
179.2
20.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.023 ± 0.616
1.581 ± 0.454
5.116 ± 0.869
5.209 ± 1.697
4.279 ± 0.419
5.674 ± 0.676
2.977 ± 0.778
6.047 ± 0.657
5.674 ± 0.672
7.256 ± 0.34
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.953 ± 0.644
5.488 ± 0.38
5.953 ± 0.641
5.86 ± 0.593
6.605 ± 1.033
9.395 ± 1.351
6.047 ± 0.941
4.837 ± 0.72
1.767 ± 0.103
3.256 ± 0.669
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here