Giant house spider associated circular virus 3
Average proteome isoelectric point is 8.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPD9|A0A346BPD9_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 3 OX=2293290 PE=4 SV=1
MM1 pKa = 7.75 ALPDD5 pKa = 3.87 KK6 pKa = 10.73 RR7 pKa = 11.84 NAYY10 pKa = 8.38 TPQLLHH16 pKa = 6.7 ARR18 pKa = 11.84 RR19 pKa = 11.84 GSCGEE24 pKa = 3.47 QNYY27 pKa = 9.73 IKK29 pKa = 10.57 RR30 pKa = 11.84 PFPRR34 pKa = 11.84 FFTMQNEE41 pKa = 4.5 LPDD44 pKa = 4.33 SKK46 pKa = 10.78 PPTVPPSPSSRR57 pKa = 11.84 YY58 pKa = 9.66 RR59 pKa = 11.84 SWCFTKK65 pKa = 10.74 NNYY68 pKa = 9.91 AISDD72 pKa = 4.11 CDD74 pKa = 4.11 SILAIPCRR82 pKa = 11.84 YY83 pKa = 10.11 VIFGKK88 pKa = 10.33 EE89 pKa = 3.85 VAPTTGTPHH98 pKa = 5.6 LQGYY102 pKa = 10.08 ISFEE106 pKa = 4.22 SAKK109 pKa = 10.51 SRR111 pKa = 11.84 SAVAKK116 pKa = 9.61 LLPGCHH122 pKa = 6.61 LLVARR127 pKa = 11.84 GTASQNKK134 pKa = 8.28 VYY136 pKa = 10.14 CSKK139 pKa = 10.95 GGQVFEE145 pKa = 4.78 NGVLPADD152 pKa = 3.79 STVGGRR158 pKa = 11.84 MEE160 pKa = 3.85 TARR163 pKa = 11.84 WDD165 pKa = 4.09 NTWSLATSGRR175 pKa = 11.84 IEE177 pKa = 5.08 EE178 pKa = 4.19 IDD180 pKa = 3.06 SDD182 pKa = 3.37 IRR184 pKa = 11.84 LRR186 pKa = 11.84 YY187 pKa = 7.41 YY188 pKa = 7.98 TTIKK192 pKa = 10.3 KK193 pKa = 10.14 IGVDD197 pKa = 3.19 YY198 pKa = 9.63 MPPVKK203 pKa = 10.41 SLEE206 pKa = 4.43 SVCGVWIYY214 pKa = 10.71 GASGAGKK221 pKa = 7.84 TRR223 pKa = 11.84 AVLASFPEE231 pKa = 4.61 AYY233 pKa = 9.54 IKK235 pKa = 9.97 PRR237 pKa = 11.84 NVWWDD242 pKa = 3.76 GYY244 pKa = 7.13 QQEE247 pKa = 4.59 EE248 pKa = 5.02 VVLLDD253 pKa = 5.04 DD254 pKa = 3.91 VDD256 pKa = 4.34 KK257 pKa = 11.32 FDD259 pKa = 3.61 VALGGKK265 pKa = 8.96 LKK267 pKa = 10.19 HH268 pKa = 6.22 WADD271 pKa = 4.47 FPPFIGEE278 pKa = 3.62 IKK280 pKa = 10.42 GISRR284 pKa = 11.84 RR285 pKa = 11.84 IRR287 pKa = 11.84 PSKK290 pKa = 10.78 LIVTSQYY297 pKa = 11.39 KK298 pKa = 8.89 MEE300 pKa = 5.34 EE301 pKa = 3.83 IWNDD305 pKa = 3.29 AQTLDD310 pKa = 3.39 ALGRR314 pKa = 11.84 RR315 pKa = 11.84 FKK317 pKa = 11.0 VIEE320 pKa = 4.56 KK321 pKa = 10.38 IKK323 pKa = 10.65 GQDD326 pKa = 3.06 IILL329 pKa = 4.05
Molecular weight: 36.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.657
IPC2_protein 8.551
IPC_protein 8.448
Toseland 8.975
ProMoST 8.931
Dawson 9.341
Bjellqvist 9.238
Wikipedia 9.502
Rodwell 9.502
Grimsley 9.355
Solomon 9.414
Lehninger 9.37
Nozaki 9.355
DTASelect 9.121
Thurlkill 9.238
EMBOSS 9.487
Sillero 9.414
Patrickios 4.596
IPC_peptide 9.399
IPC2_peptide 8.009
IPC2.peptide.svr19 7.703
Protein with the highest isoelectric point:
>tr|A0A346BPD9|A0A346BPD9_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 3 OX=2293290 PE=4 SV=1
MM1 pKa = 7.28 AAKK4 pKa = 9.83 LASAAWRR11 pKa = 11.84 NRR13 pKa = 11.84 KK14 pKa = 9.43 AISAAAAVPAAFFAGRR30 pKa = 11.84 KK31 pKa = 6.18 NTIKK35 pKa = 10.59 RR36 pKa = 11.84 KK37 pKa = 8.44 ATSQGGRR44 pKa = 11.84 PAKK47 pKa = 9.86 KK48 pKa = 9.85 AKK50 pKa = 7.93 QAKK53 pKa = 9.24 RR54 pKa = 11.84 GTRR57 pKa = 11.84 RR58 pKa = 11.84 ASSGINVEE66 pKa = 4.2 GTINTGKK73 pKa = 10.52 KK74 pKa = 8.81 IQYY77 pKa = 10.46 KK78 pKa = 8.69 NVKK81 pKa = 9.2 FNKK84 pKa = 9.71 SLQLQGGVYY93 pKa = 9.98 RR94 pKa = 11.84 KK95 pKa = 9.1 EE96 pKa = 3.9 RR97 pKa = 11.84 YY98 pKa = 6.55 EE99 pKa = 4.13 TGVLYY104 pKa = 10.38 HH105 pKa = 7.49 DD106 pKa = 5.14 PGDD109 pKa = 4.77 DD110 pKa = 3.74 IFTQKK115 pKa = 10.29 VKK117 pKa = 10.15 TIDD120 pKa = 3.54 FVFDD124 pKa = 3.76 GGPPVALGTNITSIFNDD141 pKa = 3.69 MYY143 pKa = 11.48 ANQATTAPLYY153 pKa = 10.76 SSGAVVLNQNEE164 pKa = 3.98 ALKK167 pKa = 10.83 FFLQSGSIDD176 pKa = 3.75 LEE178 pKa = 4.32 LTNMSAGSAQLTIYY192 pKa = 11.2 VLMAKK197 pKa = 7.78 NTKK200 pKa = 8.75 STTSGPTADD209 pKa = 3.55 WTNGLIAEE217 pKa = 5.1 KK218 pKa = 10.25 GSQANTPTPFTINNRR233 pKa = 11.84 PTSSKK238 pKa = 10.36 VFNVNWKK245 pKa = 8.93 VCDD248 pKa = 3.6 SKK250 pKa = 10.23 TYY252 pKa = 10.44 NAGPGAKK259 pKa = 9.56 VNYY262 pKa = 9.61 AFNFSPRR269 pKa = 11.84 SVVDD273 pKa = 2.91 ATYY276 pKa = 9.81 FARR279 pKa = 11.84 HH280 pKa = 4.17 TQIRR284 pKa = 11.84 GLTYY288 pKa = 10.38 ALLAVTHH295 pKa = 6.03 GQLGLTAAQAVVPKK309 pKa = 9.05 PVQWIYY315 pKa = 10.94 SIKK318 pKa = 9.21 KK319 pKa = 7.68 TYY321 pKa = 9.69 ILKK324 pKa = 8.82 TVNNYY329 pKa = 8.84 PAIVSQYY336 pKa = 8.17 VQSSATVSASGPVTVRR352 pKa = 11.84 EE353 pKa = 4.15 DD354 pKa = 3.63 DD355 pKa = 4.15 GEE357 pKa = 4.57 LEE359 pKa = 4.29 VV360 pKa = 4.97
Molecular weight: 38.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.134
IPC2_protein 9.487
IPC_protein 9.443
Toseland 10.131
ProMoST 9.78
Dawson 10.306
Bjellqvist 9.955
Wikipedia 10.467
Rodwell 10.847
Grimsley 10.379
Solomon 10.321
Lehninger 10.292
Nozaki 10.101
DTASelect 9.955
Thurlkill 10.16
EMBOSS 10.526
Sillero 10.218
Patrickios 10.467
IPC_peptide 10.335
IPC2_peptide 8.478
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
689
329
360
344.5
37.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.579 ± 1.803
1.161 ± 0.673
4.354 ± 0.778
3.628 ± 0.86
3.774 ± 0.088
7.547 ± 0.036
1.016 ± 0.139
5.951 ± 0.725
7.547 ± 0.388
6.386 ± 0.421
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.306 ± 0.149
4.935 ± 1.32
5.515 ± 0.816
4.064 ± 0.502
5.37 ± 0.917
7.547 ± 0.036
7.257 ± 1.456
7.112 ± 0.508
1.742 ± 0.481
4.209 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here