Acidipila rosea

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Acidipila

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3487 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1LAX1|A0A4R1LAX1_9BACT Uncharacterized protein OS=Acidipila rosea OX=768535 GN=C7378_0468 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 5.22SFSSLSQSGSANFFQAPSDD21 pKa = 3.9FADD24 pKa = 3.6TRR26 pKa = 11.84SMGTVGLSFGSPTSGTGFNVTSTVNQIVTNMQTVEE61 pKa = 4.6TPWKK65 pKa = 7.88TQLTSLQAEE74 pKa = 4.46DD75 pKa = 3.71TALTSIGTDD84 pKa = 3.52LNALSTSMQALTDD97 pKa = 3.94FQGVLAGKK105 pKa = 9.81QGSSSDD111 pKa = 3.71TNTLQLTSAATTAIAGSHH129 pKa = 6.1TIVVSQLAQTSSYY142 pKa = 11.07VSGAIANASDD152 pKa = 3.66TLAGSLTLSIGSGSSQTITLNSSDD176 pKa = 3.53NTLAGLANTINSGSYY191 pKa = 10.11GVTANVITDD200 pKa = 3.5SSGSRR205 pKa = 11.84LSLVSSTSGAGGDD218 pKa = 3.45ITLSSSMTDD227 pKa = 3.16TSNSNAAVTFTQAQAGQDD245 pKa = 3.11ALMTVDD251 pKa = 3.98GVSVTSASNTVTNAIQGVTFQLLKK275 pKa = 10.76ASPSEE280 pKa = 3.91NVQVQITNDD289 pKa = 3.36NTSVTTAVNTFVTAYY304 pKa = 10.66NKK306 pKa = 10.01VLTDD310 pKa = 4.59LNTQEE315 pKa = 5.28GNTSSGTPEE324 pKa = 3.99PLFGNPVLANLQQQLQAALTFTQPAQAVATNTTIGTSDD362 pKa = 3.43TLAGSLSIAVGTGAAQTVAVPSSTPTLAGLASAINAANMGVTANVITSGSQSTLSLQNATSGSAGVININSSSLSDD438 pKa = 3.39TTTGQAVTFGGSLSAGVTSLTQLGITVNNDD468 pKa = 2.06GTLALNGDD476 pKa = 4.27TLNSEE481 pKa = 4.7LNSNYY486 pKa = 9.72QDD488 pKa = 2.94VVNFFQPSGNFTSFGGNFSTVMGNLGNSAPSGAVYY523 pKa = 10.47LAMQEE528 pKa = 4.0NSTTEE533 pKa = 3.93SQLNTNVSNEE543 pKa = 3.77EE544 pKa = 4.3AIINTQKK551 pKa = 10.21ATLTTEE557 pKa = 4.59LNQANFTLTQIPQQLNYY574 pKa = 9.99MNEE577 pKa = 4.26LYY579 pKa = 10.8SAITGYY585 pKa = 8.17NTYY588 pKa = 10.57HH589 pKa = 6.97PP590 pKa = 4.67

Molecular weight: 59.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1L3X6|A0A4R1L3X6_9BACT Nucleotide-binding universal stress UspA family protein OS=Acidipila rosea OX=768535 GN=C7378_2345 PE=3 SV=1
MM1 pKa = 7.89PEE3 pKa = 3.96LPEE6 pKa = 4.22VEE8 pKa = 4.44TVANGVHH15 pKa = 5.41EE16 pKa = 5.39RR17 pKa = 11.84IHH19 pKa = 6.29GSRR22 pKa = 11.84VEE24 pKa = 4.04QAWFGSKK31 pKa = 10.25PEE33 pKa = 4.14PFKK36 pKa = 10.91TPPAEE41 pKa = 3.66MAAAITGATIDD52 pKa = 3.4RR53 pKa = 11.84VHH55 pKa = 6.88RR56 pKa = 11.84VGKK59 pKa = 10.01HH60 pKa = 3.95IVFDD64 pKa = 3.92LTPAPPASGSLQWIVHH80 pKa = 6.25LGMTGRR86 pKa = 11.84LLVSTPDD93 pKa = 3.58TPVPAHH99 pKa = 5.32THH101 pKa = 5.94ALLTLDD107 pKa = 3.74SGRR110 pKa = 11.84EE111 pKa = 3.87LRR113 pKa = 11.84FVDD116 pKa = 3.32ARR118 pKa = 11.84RR119 pKa = 11.84FGRR122 pKa = 11.84LALQRR127 pKa = 11.84TAFTGPGAEE136 pKa = 4.6PLTISADD143 pKa = 3.51DD144 pKa = 3.82FVALFRR150 pKa = 11.84GRR152 pKa = 11.84KK153 pKa = 8.77LAIKK157 pKa = 10.18SALLNQRR164 pKa = 11.84LLHH167 pKa = 5.82GVGNIYY173 pKa = 10.78ADD175 pKa = 3.41EE176 pKa = 4.3SLFRR180 pKa = 11.84AGIRR184 pKa = 11.84PTHH187 pKa = 5.37IAGRR191 pKa = 11.84LSRR194 pKa = 11.84PRR196 pKa = 11.84LQRR199 pKa = 11.84LHH201 pKa = 6.05TEE203 pKa = 3.91LRR205 pKa = 11.84AVLRR209 pKa = 11.84NAIALGGSSVSDD221 pKa = 3.57YY222 pKa = 11.52VDD224 pKa = 3.5ADD226 pKa = 3.53GVRR229 pKa = 11.84GFFQLEE235 pKa = 3.77HH236 pKa = 6.45RR237 pKa = 11.84VYY239 pKa = 10.67QRR241 pKa = 11.84GGQPCLVCGTAIRR254 pKa = 11.84RR255 pKa = 11.84TVLGGRR261 pKa = 11.84STHH264 pKa = 5.38WCPRR268 pKa = 11.84CQSS271 pKa = 3.2

Molecular weight: 29.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3487

0

3487

1276136

29

2650

366.0

40.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.004 ± 0.051

0.86 ± 0.013

4.993 ± 0.028

5.574 ± 0.045

3.838 ± 0.025

7.85 ± 0.045

2.396 ± 0.02

5.102 ± 0.027

3.614 ± 0.034

10.254 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.017

3.278 ± 0.038

5.274 ± 0.029

4.077 ± 0.031

6.356 ± 0.041

6.3 ± 0.035

5.689 ± 0.036

6.992 ± 0.027

1.363 ± 0.018

2.86 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski