Beihai weivirus-like virus 20
Average proteome isoelectric point is 7.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLD3|A0A1L3KLD3_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 20 OX=1922749 PE=4 SV=1
MM1 pKa = 7.62 AEE3 pKa = 3.41 AAEE6 pKa = 4.19 RR7 pKa = 11.84 VNAQQAAFRR16 pKa = 11.84 DD17 pKa = 4.23 GTTITSTVVEE27 pKa = 4.43 APGAASTSGQAEE39 pKa = 4.13 GAEE42 pKa = 4.26 VGTRR46 pKa = 11.84 VARR49 pKa = 11.84 PRR51 pKa = 11.84 FPSMSEE57 pKa = 3.75 EE58 pKa = 4.04 EE59 pKa = 4.42 VFLFSNDD66 pKa = 3.08 PRR68 pKa = 11.84 NLEE71 pKa = 3.93 AANTLRR77 pKa = 11.84 NKK79 pKa = 10.3 GVGVHH84 pKa = 5.48 QPSEE88 pKa = 4.07 KK89 pKa = 9.78 EE90 pKa = 3.55 AAVRR94 pKa = 11.84 DD95 pKa = 3.85 EE96 pKa = 5.37 LVDD99 pKa = 3.67 VLCDD103 pKa = 3.26 MVFTKK108 pKa = 10.59 RR109 pKa = 11.84 EE110 pKa = 4.02 SEE112 pKa = 4.16 KK113 pKa = 11.39 AMMEE117 pKa = 4.17 YY118 pKa = 10.75 EE119 pKa = 4.5 SLTKK123 pKa = 9.8 TALPRR128 pKa = 11.84 KK129 pKa = 9.72 LSEE132 pKa = 4.06 EE133 pKa = 4.39 QKK135 pKa = 10.93 LQWQQDD141 pKa = 3.3 ALAAAEE147 pKa = 4.44 GDD149 pKa = 3.53 GLSYY153 pKa = 11.34 SKK155 pKa = 10.91 FIDD158 pKa = 3.5 AFVKK162 pKa = 10.89 AEE164 pKa = 3.97 VSAKK168 pKa = 10.0 PKK170 pKa = 9.53 PRR172 pKa = 11.84 PIANHH177 pKa = 5.93 KK178 pKa = 9.6 EE179 pKa = 3.51 IRR181 pKa = 11.84 LCALAKK187 pKa = 10.08 CAWTYY192 pKa = 8.9 EE193 pKa = 4.17 HH194 pKa = 7.06 VLFRR198 pKa = 11.84 RR199 pKa = 11.84 MNKK202 pKa = 9.1 MSIKK206 pKa = 10.31 HH207 pKa = 4.63 RR208 pKa = 11.84 TKK210 pKa = 10.7 SEE212 pKa = 3.91 ALSDD216 pKa = 3.71 VANALSSMKK225 pKa = 10.39 NGRR228 pKa = 11.84 WCEE231 pKa = 3.69 NDD233 pKa = 2.77 LTAFEE238 pKa = 4.84 FGISAKK244 pKa = 10.58 LKK246 pKa = 9.75 ACEE249 pKa = 3.9 CAILRR254 pKa = 11.84 HH255 pKa = 5.45 VASQIGIEE263 pKa = 4.41 DD264 pKa = 3.59 VGSLLFEE271 pKa = 3.78 RR272 pKa = 11.84 VVNDD276 pKa = 3.47 RR277 pKa = 11.84 TKK279 pKa = 8.78 TCVWSMRR286 pKa = 11.84 YY287 pKa = 7.83 TDD289 pKa = 3.38 EE290 pKa = 4.31 TGEE293 pKa = 3.99 KK294 pKa = 9.02 RR295 pKa = 11.84 TFRR298 pKa = 11.84 LILPTVMRR306 pKa = 11.84 EE307 pKa = 3.8 SGDD310 pKa = 3.56 RR311 pKa = 11.84 LTSSGNFLQNLIAWCSFLLAPGTVRR336 pKa = 11.84 KK337 pKa = 9.9 SVEE340 pKa = 3.74 SLLRR344 pKa = 11.84 TRR346 pKa = 11.84 GEE348 pKa = 3.9 HH349 pKa = 5.77 MFYY352 pKa = 10.88 CSARR356 pKa = 11.84 DD357 pKa = 3.6 GKK359 pKa = 10.95 KK360 pKa = 10.31 YY361 pKa = 9.81 LAKK364 pKa = 10.51 LVFEE368 pKa = 5.28 GDD370 pKa = 3.58 DD371 pKa = 3.31 TLGRR375 pKa = 11.84 LDD377 pKa = 3.75 EE378 pKa = 4.74 PVWEE382 pKa = 4.82 PKK384 pKa = 10.16 RR385 pKa = 11.84 EE386 pKa = 3.97 GATEE390 pKa = 4.01 SLVDD394 pKa = 3.62 DD395 pKa = 4.96 FFYY398 pKa = 10.79 RR399 pKa = 11.84 WGWKK403 pKa = 9.42 PKK405 pKa = 10.27 LSWKK409 pKa = 8.81 ATSGYY414 pKa = 10.86 DD415 pKa = 3.29 YY416 pKa = 11.33 ARR418 pKa = 11.84 VVGYY422 pKa = 10.35 DD423 pKa = 3.2 ILIKK427 pKa = 10.89 DD428 pKa = 4.11 GVAVRR433 pKa = 11.84 DD434 pKa = 3.54 GDD436 pKa = 4.03 KK437 pKa = 10.97 LVACPEE443 pKa = 3.76 MRR445 pKa = 11.84 RR446 pKa = 11.84 LLNTKK451 pKa = 8.13 QWTTTNVTPEE461 pKa = 3.93 EE462 pKa = 4.3 LKK464 pKa = 8.58 TCNRR468 pKa = 11.84 IFAATLAADD477 pKa = 4.67 FTRR480 pKa = 11.84 VEE482 pKa = 4.01 PMYY485 pKa = 11.06 SFLRR489 pKa = 11.84 SMYY492 pKa = 10.49 DD493 pKa = 3.23 SNPGGKK499 pKa = 7.54 TVTDD503 pKa = 3.56 EE504 pKa = 4.02 KK505 pKa = 11.0 VRR507 pKa = 11.84 EE508 pKa = 4.33 HH509 pKa = 6.5 FLMMTGEE516 pKa = 4.28 LPAHH520 pKa = 6.56 GSSLLSDD527 pKa = 3.36 IAFPEE532 pKa = 3.92 FDD534 pKa = 3.25 GAGNDD539 pKa = 4.03 VEE541 pKa = 4.58 WKK543 pKa = 9.67 ALARR547 pKa = 11.84 VSCGDD552 pKa = 3.49 FTDD555 pKa = 5.5 LEE557 pKa = 4.46 WATACAQPLHH567 pKa = 6.83 DD568 pKa = 4.48 RR569 pKa = 11.84 HH570 pKa = 6.16 GADD573 pKa = 4.65 LATGMPASWVGTVAA587 pKa = 4.68
Molecular weight: 65.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.772
IPC2_protein 5.792
IPC_protein 5.817
Toseland 6.033
ProMoST 6.109
Dawson 6.02
Bjellqvist 6.046
Wikipedia 5.995
Rodwell 5.995
Grimsley 6.109
Solomon 6.02
Lehninger 6.008
Nozaki 6.262
DTASelect 6.427
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.364
Patrickios 4.431
IPC_peptide 6.033
IPC2_peptide 6.326
IPC2.peptide.svr19 6.37
Protein with the highest isoelectric point:
>tr|A0A1L3KLD3|A0A1L3KLD3_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 20 OX=1922749 PE=4 SV=1
MM1 pKa = 7.55 AGKK4 pKa = 8.56 GTRR7 pKa = 11.84 KK8 pKa = 9.98 NGNNARR14 pKa = 11.84 RR15 pKa = 11.84 APRR18 pKa = 11.84 RR19 pKa = 11.84 QGGRR23 pKa = 11.84 AANRR27 pKa = 11.84 STATRR32 pKa = 11.84 VLATGVGASVPKK44 pKa = 10.36 PFGSRR49 pKa = 11.84 PGQGLACWDD58 pKa = 3.8 AKK60 pKa = 10.85 HH61 pKa = 6.5 PSHH64 pKa = 7.04 LALPRR69 pKa = 11.84 AVGPYY74 pKa = 5.72 TTVRR78 pKa = 11.84 ATRR81 pKa = 11.84 RR82 pKa = 11.84 VQLNTHH88 pKa = 6.44 CNVIGTFQEE97 pKa = 4.01 GHH99 pKa = 6.54 IAPSQAGDD107 pKa = 3.03 WSEE110 pKa = 4.14 VVLVADD116 pKa = 4.09 VTATNPINGHH126 pKa = 6.07 LNTRR130 pKa = 11.84 SVTVDD135 pKa = 3.22 LSGLGDD141 pKa = 3.88 AATLVPSALSVQVMCPTALQTASGIIYY168 pKa = 9.82 AGVMNTQAALGGRR181 pKa = 11.84 VDD183 pKa = 5.98 SWDD186 pKa = 3.26 DD187 pKa = 3.03 WCNKK191 pKa = 8.38 FVQFQSPRR199 pKa = 11.84 LLAASKK205 pKa = 10.23 LALRR209 pKa = 11.84 GIQINSYY216 pKa = 8.39 PLNMTEE222 pKa = 4.06 VSKK225 pKa = 8.46 FTPLRR230 pKa = 11.84 KK231 pKa = 9.37 EE232 pKa = 3.51 ADD234 pKa = 3.55 LQFEE238 pKa = 4.53 YY239 pKa = 10.66 TDD241 pKa = 3.36 QRR243 pKa = 11.84 VEE245 pKa = 3.98 PTGWAPIVIYY255 pKa = 10.64 NPEE258 pKa = 4.03 GTTLEE263 pKa = 4.02 LLITVEE269 pKa = 3.69 YY270 pKa = 10.11 RR271 pKa = 11.84 VRR273 pKa = 11.84 FDD275 pKa = 4.28 LDD277 pKa = 3.4 HH278 pKa = 7.02 PASASHH284 pKa = 5.36 THH286 pKa = 6.25 HH287 pKa = 6.96 PVASDD292 pKa = 3.69 STWDD296 pKa = 3.3 RR297 pKa = 11.84 MVRR300 pKa = 11.84 SATALGNGVMDD311 pKa = 4.02 IADD314 pKa = 3.62 VVANTGMAVGRR325 pKa = 11.84 AAMVGRR331 pKa = 11.84 ALTNGAARR339 pKa = 11.84 ALPALTMM346 pKa = 4.44
Molecular weight: 36.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.014
IPC2_protein 8.99
IPC_protein 9.136
Toseland 9.619
ProMoST 9.458
Dawson 9.897
Bjellqvist 9.692
Wikipedia 10.101
Rodwell 10.043
Grimsley 9.984
Solomon 9.955
Lehninger 9.926
Nozaki 9.794
DTASelect 9.633
Thurlkill 9.75
EMBOSS 10.058
Sillero 9.867
Patrickios 5.601
IPC_peptide 9.955
IPC2_peptide 8.668
IPC2.peptide.svr19 7.907
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
933
346
587
466.5
51.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.468 ± 0.869
1.822 ± 0.376
5.466 ± 0.476
6.109 ± 1.983
3.43 ± 0.795
7.181 ± 0.842
2.036 ± 0.319
2.787 ± 0.222
4.716 ± 1.359
8.467 ± 0.212
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.787 ± 0.105
3.751 ± 0.657
4.502 ± 0.723
2.68 ± 0.609
7.181 ± 0.188
6.538 ± 0.265
7.503 ± 0.66
7.61 ± 0.763
2.036 ± 0.171
1.929 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here