Litorimonas taeanensis
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2552 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A420WDM2|A0A420WDM2_9PROT RHS repeat-associated protein OS=Litorimonas taeanensis OX=568099 GN=DES40_1920 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.2 FLKK5 pKa = 8.23 TTIATLALTGFAGQAMAQDD24 pKa = 3.74 TGAYY28 pKa = 6.7 GTLGVSTYY36 pKa = 11.05 EE37 pKa = 3.52 FDD39 pKa = 3.55 TYY41 pKa = 11.34 NITGRR46 pKa = 11.84 LGYY49 pKa = 10.27 NLNEE53 pKa = 3.91 YY54 pKa = 10.43 FGIEE58 pKa = 4.25 GEE60 pKa = 4.29 GSIGVIGEE68 pKa = 4.18 DD69 pKa = 3.65 DD70 pKa = 3.79 NGVEE74 pKa = 6.11 VDD76 pKa = 3.96 TEE78 pKa = 4.13 WDD80 pKa = 3.14 LGAYY84 pKa = 9.15 AVARR88 pKa = 11.84 LPLSNQFEE96 pKa = 4.38 AFVRR100 pKa = 11.84 GGYY103 pKa = 7.63 TVVSVEE109 pKa = 3.88 ATVAGVTDD117 pKa = 3.52 SDD119 pKa = 4.0 EE120 pKa = 4.3 VDD122 pKa = 3.92 GYY124 pKa = 11.76 AVGGGVQYY132 pKa = 11.35 NLNDD136 pKa = 3.57 RR137 pKa = 11.84 SGLRR141 pKa = 11.84 LGYY144 pKa = 8.64 TYY146 pKa = 11.2 KK147 pKa = 10.99 DD148 pKa = 3.17 GDD150 pKa = 4.25 LNGVDD155 pKa = 5.04 LDD157 pKa = 4.18 ADD159 pKa = 3.76 VWDD162 pKa = 3.6 VSYY165 pKa = 11.32 VRR167 pKa = 11.84 KK168 pKa = 9.65 FF169 pKa = 3.06
Molecular weight: 18.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.554
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.986
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A420WE23|A0A420WE23_9PROT Peptidase M28-like protein OS=Litorimonas taeanensis OX=568099 GN=DES40_1947 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.29 AGRR28 pKa = 11.84 QILSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2552
0
2552
835630
29
3046
327.4
35.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.013 ± 0.057
0.84 ± 0.014
6.038 ± 0.038
6.14 ± 0.052
4.07 ± 0.042
7.812 ± 0.051
1.878 ± 0.024
6.222 ± 0.04
5.06 ± 0.051
9.498 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.557 ± 0.03
3.774 ± 0.036
4.548 ± 0.035
3.423 ± 0.029
5.466 ± 0.038
6.488 ± 0.036
5.713 ± 0.053
6.555 ± 0.057
1.144 ± 0.017
2.759 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here