Streptococcus satellite phage Javan732

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZV78|A0A4D5ZV78_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan732 OX=2558826 GN=JavanS732_0009 PE=4 SV=1
MM1 pKa = 7.22TLDD4 pKa = 4.01LGKK7 pKa = 8.39MTQAEE12 pKa = 4.45FDD14 pKa = 3.72EE15 pKa = 4.66VMADD19 pKa = 3.21IKK21 pKa = 11.13ARR23 pKa = 11.84NPNLLQLITDD33 pKa = 4.52FLDD36 pKa = 3.62RR37 pKa = 11.84KK38 pKa = 8.24VTPEE42 pKa = 4.05EE43 pKa = 3.86VDD45 pKa = 3.54DD46 pKa = 4.24

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZUW3|A0A4D5ZUW3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan732 OX=2558826 GN=JavanS732_0007 PE=4 SV=1
MM1 pKa = 7.27QKK3 pKa = 10.12RR4 pKa = 11.84GKK6 pKa = 9.97LKK8 pKa = 10.04NRR10 pKa = 11.84KK11 pKa = 8.65PIYY14 pKa = 9.15RR15 pKa = 11.84LKK17 pKa = 10.79RR18 pKa = 11.84KK19 pKa = 9.53IGQKK23 pKa = 9.55CNKK26 pKa = 9.51HH27 pKa = 5.42EE28 pKa = 3.89AHH30 pKa = 6.95CYY32 pKa = 8.84YY33 pKa = 10.66FSFRR37 pKa = 11.84AAEE40 pKa = 4.06SAEE43 pKa = 3.82NATYY47 pKa = 10.46SIPTRR52 pKa = 11.84HH53 pKa = 6.78NIQQSS58 pKa = 3.12

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

3710

38

496

137.4

15.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.66 ± 0.416

0.755 ± 0.135

5.768 ± 0.396

9.623 ± 0.691

4.124 ± 0.318

4.798 ± 0.301

1.429 ± 0.256

7.035 ± 0.362

9.191 ± 0.511

10.081 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.216

5.58 ± 0.385

2.965 ± 0.37

3.935 ± 0.291

5.499 ± 0.223

5.418 ± 0.348

5.472 ± 0.458

5.283 ± 0.457

0.782 ± 0.208

3.962 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski