Raspberry vein chlorosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Raspberry vein chlorosis cytorhabdovirus

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482P9Z3|A0A482P9Z3_9RHAB P6 protein OS=Raspberry vein chlorosis virus OX=758677 GN=P6 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 6.21NIDD5 pKa = 5.42FDD7 pKa = 4.82ALPNPILDD15 pKa = 3.66VAMSEE20 pKa = 4.0IDD22 pKa = 3.52QEE24 pKa = 4.57RR25 pKa = 11.84NTDD28 pKa = 3.6DD29 pKa = 4.14LVAGKK34 pKa = 10.1SRR36 pKa = 11.84EE37 pKa = 4.17SNMGRR42 pKa = 11.84IEE44 pKa = 4.11EE45 pKa = 4.31SGQEE49 pKa = 4.1VAEE52 pKa = 4.24KK53 pKa = 10.21QSKK56 pKa = 10.44LEE58 pKa = 3.82SDD60 pKa = 3.58GAYY63 pKa = 10.14QEE65 pKa = 5.54DD66 pKa = 3.8IPYY69 pKa = 10.61DD70 pKa = 3.87RR71 pKa = 11.84NDD73 pKa = 3.29IDD75 pKa = 3.77TALTDD80 pKa = 4.13LQHH83 pKa = 6.88LCDD86 pKa = 4.26AMGVNYY92 pKa = 7.83TIPMEE97 pKa = 4.42NQVKK101 pKa = 9.32MLFRR105 pKa = 11.84DD106 pKa = 3.61EE107 pKa = 4.42EE108 pKa = 4.19VCYY111 pKa = 9.53THH113 pKa = 7.37LIWYY117 pKa = 8.99LRR119 pKa = 11.84GIINANQTQIIPTITGAISDD139 pKa = 3.99MKK141 pKa = 10.57MEE143 pKa = 4.15TRR145 pKa = 11.84QLQASSNKK153 pKa = 9.63LSKK156 pKa = 9.48EE157 pKa = 3.81TANIEE162 pKa = 4.2KK163 pKa = 9.68TSKK166 pKa = 10.55SLYY169 pKa = 10.4DD170 pKa = 3.85EE171 pKa = 3.86IRR173 pKa = 11.84AIKK176 pKa = 10.31EE177 pKa = 3.7DD178 pKa = 3.42MKK180 pKa = 11.12EE181 pKa = 3.98SFRR184 pKa = 11.84ASMKK188 pKa = 10.71LFMEE192 pKa = 5.06EE193 pKa = 4.02VQNEE197 pKa = 4.36KK198 pKa = 8.97PTQEE202 pKa = 4.08PKK204 pKa = 10.57QPLINPSKK212 pKa = 10.48IADD215 pKa = 3.87LKK217 pKa = 10.55AVKK220 pKa = 10.42DD221 pKa = 3.91LVLNSYY227 pKa = 10.6GNAGEE232 pKa = 4.8AMIPNKK238 pKa = 10.39ADD240 pKa = 3.09IPEE243 pKa = 4.36PTHH246 pKa = 4.8TNKK249 pKa = 10.01VIDD252 pKa = 4.52EE253 pKa = 4.15YY254 pKa = 11.39HH255 pKa = 5.98KK256 pKa = 10.58EE257 pKa = 3.93KK258 pKa = 10.57RR259 pKa = 11.84DD260 pKa = 3.57ALIKK264 pKa = 10.88YY265 pKa = 9.02GVDD268 pKa = 3.12PALVKK273 pKa = 10.58SYY275 pKa = 10.46EE276 pKa = 4.12DD277 pKa = 3.56SVIDD281 pKa = 4.84AMYY284 pKa = 10.0PDD286 pKa = 5.43DD287 pKa = 4.37VHH289 pKa = 7.13AQLKK293 pKa = 10.28AIRR296 pKa = 11.84LNEE299 pKa = 4.52GIKK302 pKa = 10.02RR303 pKa = 11.84QIRR306 pKa = 11.84KK307 pKa = 9.5SLEE310 pKa = 3.72EE311 pKa = 3.91RR312 pKa = 11.84LEE314 pKa = 4.62DD315 pKa = 3.85YY316 pKa = 11.06LDD318 pKa = 4.27EE319 pKa = 4.57EE320 pKa = 5.65DD321 pKa = 4.78EE322 pKa = 4.74DD323 pKa = 5.17EE324 pKa = 4.3EE325 pKa = 4.31MSNGSIQGDD334 pKa = 3.89SYY336 pKa = 12.07ASTEE340 pKa = 3.86

Molecular weight: 38.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482PGG3|A0A482PGG3_9RHAB Movement protein OS=Raspberry vein chlorosis virus OX=758677 GN=P3 PE=4 SV=1
MM1 pKa = 7.11FVDD4 pKa = 4.51VDD6 pKa = 3.81PEE8 pKa = 4.24LLFKK12 pKa = 10.95LFSRR16 pKa = 11.84FISPNFLRR24 pKa = 11.84WTILILTLYY33 pKa = 8.49QTLSLMWQCLKK44 pKa = 10.65LIKK47 pKa = 10.47SVIQMILWLAKK58 pKa = 9.97AGRR61 pKa = 11.84AIWAGLKK68 pKa = 10.43NLGKK72 pKa = 10.36RR73 pKa = 11.84LRR75 pKa = 11.84RR76 pKa = 11.84SKK78 pKa = 11.18ASS80 pKa = 3.16

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4072

65

2092

509.0

57.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.231 ± 1.318

1.817 ± 0.354

6.238 ± 0.656

5.968 ± 0.744

2.947 ± 0.332

5.869 ± 0.628

2.112 ± 0.329

7.22 ± 0.458

6.68 ± 0.338

9.234 ± 0.757

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.88 ± 0.259

4.47 ± 0.34

4.052 ± 0.232

2.849 ± 0.464

5.82 ± 0.381

8.374 ± 0.435

6.164 ± 0.393

5.918 ± 0.385

1.523 ± 0.259

3.635 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski