Infectious hematopoietic necrosis virus (strain WRAC) (IHNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae; Novirhabdovirus; Salmonid novirhabdovirus; Infectious hematopoietic necrosis virus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q82681|PHOSP_IHNVW Phosphoprotein OS=Infectious hematopoietic necrosis virus (strain WRAC) OX=429314 GN=P PE=3 SV=1
MM1 pKa = 7.27TSALRR6 pKa = 11.84EE7 pKa = 4.31TFTGLRR13 pKa = 11.84DD14 pKa = 3.4IKK16 pKa = 11.01GGVLEE21 pKa = 5.08DD22 pKa = 4.37PEE24 pKa = 4.5TEE26 pKa = 4.17YY27 pKa = 11.12RR28 pKa = 11.84PSTITLPLFFSKK40 pKa = 9.93TDD42 pKa = 3.96LDD44 pKa = 3.96LEE46 pKa = 4.45MIKK49 pKa = 10.41RR50 pKa = 11.84AVSQVGGEE58 pKa = 4.35GTRR61 pKa = 11.84KK62 pKa = 9.93ALSLLCAFVIAEE74 pKa = 4.46TVPSEE79 pKa = 4.18AGTVAEE85 pKa = 4.58LLEE88 pKa = 4.18ALGFVLEE95 pKa = 4.52SLDD98 pKa = 3.49TGAPPDD104 pKa = 3.76ATFADD109 pKa = 4.49PNNKK113 pKa = 9.42LAEE116 pKa = 4.54TIVKK120 pKa = 10.04EE121 pKa = 4.02NVLEE125 pKa = 4.28VVTGLLFTCALLTKK139 pKa = 10.44YY140 pKa = 10.51DD141 pKa = 3.65VDD143 pKa = 5.43KK144 pKa = 10.91MATYY148 pKa = 9.75CQNKK152 pKa = 9.71LEE154 pKa = 4.45RR155 pKa = 11.84LATSQGIGEE164 pKa = 4.45LVNFNANRR172 pKa = 11.84GVLAKK177 pKa = 9.98IGAVLRR183 pKa = 11.84PGQKK187 pKa = 8.37LTKK190 pKa = 9.73AIYY193 pKa = 9.93GIILINLSDD202 pKa = 3.73PAIAARR208 pKa = 11.84AKK210 pKa = 10.03ALCAMRR216 pKa = 11.84LSGTGMTMVGLFNQAAKK233 pKa = 10.88NLGALPADD241 pKa = 4.15LLEE244 pKa = 4.56DD245 pKa = 3.65LCMKK249 pKa = 10.5SVVEE253 pKa = 3.85SARR256 pKa = 11.84RR257 pKa = 11.84IVRR260 pKa = 11.84LMRR263 pKa = 11.84IVAEE267 pKa = 4.31APGVAAKK274 pKa = 10.28YY275 pKa = 9.64GVMMSRR281 pKa = 11.84MLGEE285 pKa = 5.19GYY287 pKa = 10.46FKK289 pKa = 10.86AYY291 pKa = 10.1GINEE295 pKa = 4.17NARR298 pKa = 11.84ITCILMNINDD308 pKa = 4.56RR309 pKa = 11.84YY310 pKa = 11.36DD311 pKa = 3.95DD312 pKa = 3.92GTSRR316 pKa = 11.84GLTGIKK322 pKa = 10.33VSDD325 pKa = 4.27PFRR328 pKa = 11.84KK329 pKa = 9.43LARR332 pKa = 11.84EE333 pKa = 3.85IARR336 pKa = 11.84LLVLKK341 pKa = 10.68YY342 pKa = 10.73DD343 pKa = 4.17GDD345 pKa = 4.03GSTGEE350 pKa = 4.37GASEE354 pKa = 4.73LIRR357 pKa = 11.84RR358 pKa = 11.84AEE360 pKa = 3.99MASRR364 pKa = 11.84GPDD367 pKa = 2.89MGEE370 pKa = 3.74EE371 pKa = 4.11EE372 pKa = 4.82EE373 pKa = 4.79EE374 pKa = 4.85DD375 pKa = 4.39EE376 pKa = 4.91EE377 pKa = 6.79DD378 pKa = 5.06DD379 pKa = 5.57DD380 pKa = 5.65SSEE383 pKa = 4.67PGDD386 pKa = 3.5SDD388 pKa = 4.46SFHH391 pKa = 6.93

Molecular weight: 42.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q82683|GLYCO_IHNVW Glycoprotein OS=Infectious hematopoietic necrosis virus (strain WRAC) OX=429314 GN=G PE=3 SV=1
MM1 pKa = 7.66SIFKK5 pKa = 10.15RR6 pKa = 11.84AKK8 pKa = 8.38KK9 pKa = 8.4TVLIPPPHH17 pKa = 6.69LLSGDD22 pKa = 3.82EE23 pKa = 4.03EE24 pKa = 4.63RR25 pKa = 11.84VTILSAEE32 pKa = 4.3GEE34 pKa = 4.21IKK36 pKa = 9.74VTGRR40 pKa = 11.84RR41 pKa = 11.84PTTLEE46 pKa = 3.5EE47 pKa = 4.2KK48 pKa = 9.99IYY50 pKa = 11.25YY51 pKa = 10.45SMNLAAAIVGGDD63 pKa = 3.31LHH65 pKa = 7.04PSFKK69 pKa = 11.13SMTYY73 pKa = 10.43LFQKK77 pKa = 10.35EE78 pKa = 4.28MEE80 pKa = 4.5FGSTQEE86 pKa = 3.99KK87 pKa = 10.7VNFGSRR93 pKa = 11.84KK94 pKa = 8.71PAPQTTYY101 pKa = 10.34QVTKK105 pKa = 10.32ARR107 pKa = 11.84EE108 pKa = 4.32VYY110 pKa = 10.51LQTQPLEE117 pKa = 4.38KK118 pKa = 9.74KK119 pKa = 10.35IPMQTYY125 pKa = 9.21SVSTEE130 pKa = 3.61GATITFTGRR139 pKa = 11.84FLFSSSHH146 pKa = 6.01VGCDD150 pKa = 3.73DD151 pKa = 4.41NRR153 pKa = 11.84TKK155 pKa = 10.94LAGLDD160 pKa = 3.59GFTTSNSYY168 pKa = 10.23QRR170 pKa = 11.84VKK172 pKa = 10.7DD173 pKa = 3.79YY174 pKa = 11.04YY175 pKa = 10.56AQEE178 pKa = 3.85TALALTFAAPEE189 pKa = 3.83KK190 pKa = 10.28RR191 pKa = 11.84GKK193 pKa = 10.02EE194 pKa = 3.92KK195 pKa = 10.97

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3421

111

1986

570.2

64.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.782 ± 0.737

1.432 ± 0.367

5.817 ± 0.423

7.279 ± 0.723

3.712 ± 0.216

6.109 ± 0.586

2.163 ± 0.4

6.665 ± 0.763

6.226 ± 0.562

10.932 ± 1.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.303

3.537 ± 0.289

4.443 ± 0.338

3.391 ± 0.583

6.402 ± 0.591

7.395 ± 0.642

6.519 ± 0.66

4.969 ± 0.253

1.257 ± 0.395

2.631 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski