Escherichia phage vB_EcoS_ACG-M12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Guelphvirus; Escherichia virus ACGM12

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4FBU4|K4FBU4_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_ACG-M12 OX=1141140 GN=ACG-M12_0060 PE=4 SV=1
MM1 pKa = 6.99ATIYY5 pKa = 10.37TGINYY10 pKa = 8.42EE11 pKa = 4.11SRR13 pKa = 11.84KK14 pKa = 7.94TGYY17 pKa = 9.8IYY19 pKa = 10.57EE20 pKa = 4.28LVVTEE25 pKa = 5.06DD26 pKa = 3.95GYY28 pKa = 10.92ILEE31 pKa = 5.96DD32 pKa = 3.85IDD34 pKa = 3.97DD35 pKa = 3.87SNYY38 pKa = 10.52AFEE41 pKa = 4.3VTPTYY46 pKa = 10.88KK47 pKa = 10.56DD48 pKa = 3.38DD49 pKa = 4.84LEE51 pKa = 4.56TVKK54 pKa = 10.87NAFGEE59 pKa = 4.33KK60 pKa = 9.38FDD62 pKa = 4.11EE63 pKa = 4.66VSS65 pKa = 2.91

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4FBT6|K4FBT6_9CAUD Putative minor tail protein OS=Escherichia phage vB_EcoS_ACG-M12 OX=1141140 GN=ACG-M12_0033 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84SAVAKK7 pKa = 10.44NVTKK11 pKa = 10.57EE12 pKa = 3.92SLYY15 pKa = 10.35MKK17 pKa = 10.15LRR19 pKa = 11.84HH20 pKa = 5.58LTRR23 pKa = 11.84NHH25 pKa = 6.25NLLIGDD31 pKa = 4.68IYY33 pKa = 9.31WTALPPSHH41 pKa = 7.05MINPSKK47 pKa = 10.96ANNHH51 pKa = 6.48RR52 pKa = 11.84EE53 pKa = 4.15TQNGLLHH60 pKa = 6.45HH61 pKa = 7.15

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

14003

41

1138

179.5

20.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.263 ± 0.537

1.343 ± 0.188

6.184 ± 0.237

6.656 ± 0.32

3.949 ± 0.201

7.42 ± 0.272

1.493 ± 0.179

6.82 ± 0.235

6.72 ± 0.407

6.827 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.237

5.356 ± 0.303

2.985 ± 0.211

3.621 ± 0.324

4.842 ± 0.2

6.999 ± 0.323

5.449 ± 0.301

7.213 ± 0.288

1.343 ± 0.112

3.871 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski